Human Anti-Influenza virus A IgG ELISA Kit

Product details

Human Anti-Influenza virus A IgG ELISA Kit is an indirect ELISA for the qualitative detection of IgG class antibodies against Influenza virus A in human plasma and serum samples.

How the assay works:

A 96-well plate has been precoated with Influenza virus A antigens to ind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Influenza virus A-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Influenza virus A IgG sample captured in plate.

Assay Specificity

Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details

Human Anti-Influenza virus A IgG ELISA Kit ab108745 protocol summary

1. Add samples and controls to wells used. Incubate at 37°C

2. Wash each well and add prepared labeled HRP-Conjugate. Incubate at room temperature
3. After washing, add TMB substrate solution to each well. Incubate at room temperature
4. Add Stop Solution to each well. Read immediately.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.