Human Anti-Influenza virus A IgG ELISA Kit is a Indirect ELISA kit for the measurement of Human Anti-Influenza virus A IgG in Human in Citrate plasma, Heparin Plasma, Serum samples.
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
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Human Anti-Influenza virus A IgG ELISA Kit is a Indirect ELISA kit for the measurement of Human Anti-Influenza virus A IgG in Human in Citrate plasma, Heparin Plasma, Serum samples.
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 20 | mean - | SD - | C.V. 4.5 |
Sample Serum | n 24 | mean - | SD - | C.V. 3.3 |
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 12 | mean - | SD - | C.V. 3.2 |
Sample Serum | n 12 | mean - | SD - | C.V. 2.1 |
Abcam's anti-Influenza virus A IgG Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgG class antibodies against Influenza virus A in Human serum and plasma.
A 96-well plate has been precoated with Influenza virus A antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Influenza virus A-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Influenza virus A IgG sample captured in plate.
Influenza virus A IgG is an immunoglobulin G antibody that targets influenza virus A. Immunoglobulins are critical components of the immune system often used as diagnostic markers. The mass of IgG varies due to its complex structure generally around 150 kDa. This antibody is primarily expressed by B lymphocytes in response to influenza virus infection and plays a central role in identifying and neutralizing virus particles. The utilization of IgG in applications like Influenza ELISA test and Influenza assay kits highlights its importance in laboratory settings.
The influenza virus IgG antibody binds specifically to antigens present on the influenza virus A tagging them for destruction by other immune cells. It does not typically form part of larger protein complexes but operates in concert with other components of the immune system like macrophages and natural killer cells to neutralize the virus. The presence and quantity of influenza IgG antibodies are pivotal indicators of past exposure or vaccination against flu virus A in ELISA testing.
This antibody fits into the immune system pathway where it plays a role in activating the classical complement pathway. This pathway leads to opsonization of the virus therefore improving phagocytosis efficacy by macrophages. Additionally influenza virus A IgG interacts with Fc receptors on immune effector cells facilitating processes such as antibody-dependent cellular cytotoxicity (ADCC). These interactions highlight the relationship between influenza IgG and other immune receptors in mounting an effective response.
The influenza virus A IgG is closely related to influenza a respiratory illness caused by the influenza virus A. Monitoring IgG levels can help assess immunity status and guide vaccination strategies. Furthermore disturbances in IgG production might correlate with immune-related disorders such as immunodeficiencies or allergies. The presence of IgG is pivotal for the diagnosis and management of influenza and sometimes connects to adaptive immune responses involving proteins like the T-cell receptor during immune system adaptations.
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