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AB108747

Human Anti-Influenza virus A IgM ELISA Kit

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(2 Publications)

Human Anti-Influenza virus A IgM ELISA Kit is an indirect ELISA for the qualitative detection of IgM class antibodies against Influenza virus A in human plasma and serum samples.

- Colorimetric readout - 450 nm - Works on any standard plate reader
- Easy results interpretation
- Cut-off, positive and negative controls included

Key facts

Detection method

Colorimetric

Sample types

Citrate plasma, Heparin Plasma, Serum

Reacts with

Human

Assay type

Indirect

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's anti-Influenza virus A IgM Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgM class antibodies against Influenza virus A in Human serum and plasma.

A 96-well plate has been precoated with Influenza virus A antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgM conjugate is added to the wells, which binds to the immobilized Influenza virus A-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Influenza virus A IgM sample captured in plate.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 17, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.7" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.8" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab108747-1x96Tests", "assetComponentDetails": [ { "size":"1 x 20 mL", "name":"Influenza virus A anti-IgM HRP Conjugate", "number":"AB108747-CMP06", "productcode":"" }, { "size":"1 x 100 mL", "name":"IgM Sample Diluent", "number":"AB108747-CMP01", "productcode":"" }, { "size":"1 x 2 mL", "name":"Influenza virus A IgM Positive Control", "number":"AB108747-CMP07", "productcode":"" }, { "size":"1 x 3 mL", "name":"Influenza virus A IgM Cut-off Control", "number":"AB108747-CMP08", "productcode":"" }, { "size":"1 x 2 mL", "name":"Influenza virus A IgM Negative Control", "number":"AB108747-CMP09", "productcode":"" }, { "size":"1 x 50 mL", "name":"20X Washing Solution", "number":"AB108747-CMP05", "productcode":"" }, { "size":"1 x 15 mL", "name":"Stop Solution", "number":"AB108747-CMP11", "productcode":"" }, { "size":"1 x 15 mL", "name":"TMB Substrate Solution", "number":"AB108747-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Cover foil", "number":"AB108747-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Strip holder", "number":"AB108747-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Influenza virus A (IgM) Coated Microplate (12 x 8 wells)", "number":"AB108747-CMP10", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Influenza Virus A IgM often referred to as IgM influenza is a specific class of antibodies produced by the immune system in response to an Influenza A infection. These immunoglobulins are part of the early immune response. The molecular mass of an IgM antibody approximately reaches 970 kDa. IgM antibodies are expressed on the surface of B cells and can also be found in the bloodstream. They function by binding to the Influenza A antigens paving the way for subsequent immune responses. Tests like Influenza IgM ELISA can detect their presence serving as critical diagnostic tools.
Biological function summary

IgM antibodies play a significant role in neutralizing the virus during early infection stages. When the immune system detects the Influenza A virus it initiates the expression of these antibodies. IgM is part of the adaptive immune system and forms complexes with viral antigens to inhibit their activity and facilitate their clearance. This antibody class is often the first line of defense acting before IgG antibodies take over the sustained immune response.

Pathways

Immune cells produce IgM antibodies as part of the humoral immune response. This process involves the activation of the complement system which enhances pathogen clearance. The pathways incorporate proteins like C1q in the classic complement pathway and are interlinked with other immunoglobulins such as IgG which also participates in neutralizing pathogens after the IgM response. These pathways form an elaborate network important for effective viral elimination.

IgM antibodies against Influenza A are connected to the detection and progression of influenza infections. Elevated IgM levels typically indicate a recent or active infection and help distinguish influenza from other respiratory diseases. IgM levels decline as IgG antibodies rise marking viral elimination and recovery. In some cases the immune response including IgM might connect to broader disorders such as autoimmune conditions but direct causative relations typically involve other immune components like IgA.
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Product protocols

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Target data

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in nutrition 10:1116634 PubMed37168053

2023

Healthy adults supplemented with a nutraceutical formulation containing gel, rosemary and enhances the effect of influenza vaccination in a randomized, triple-blind, placebo-controlled trial.

Applications

Unspecified application

Species

Unspecified reactive species

Erin D Lewis,David C Crowley,Najla Guthrie,Malkanthi Evans

Vaccines 10: PubMed35891309

2022

Adults with Trisomy 21 Have Differential Antibody Responses to Influenza A.

Applications

Unspecified application

Species

Unspecified reactive species

Stephanie James,Robert C Haight,Cassandra Hanna,Lindsey Furton
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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