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AB108748

Human Anti-Influenza virus B IgM ELISA Kit

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(2 Publications)

Human Anti-Influenza virus B IgM ELISA Kit is an indirect ELISA for the qualitative detection of IgM class antibodies against Influenza virus B in human plasma and serum samples.

- Colorimetric readout - 450 nm - Works on any standard plate reader
- Easy results interpretation
- Cut-off, positive and negative controls included

Key facts

Detection method

Colorimetric

Sample types

Citrate plasma, Heparin Plasma, Serum

Reacts with

Human

Assay type

Indirect

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's anti-Influenza virus B IgM Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgM class antibodies against Influenza virus B in Human serum and plasma.

A 96-well plate has been precoated with Influenza virus B antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgM conjugate is added to the wells, which binds to the immobilized Influenza virus B-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Influenza virus B IgM sample captured in plate.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 12, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.5" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.9" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab108748-1x96Tests", "assetComponentDetails": [ { "size":"1 x 20 mL", "name":"Influenza virus B anti-IgM HRP Conjugate", "number":"AB108748-CMP06", "productcode":"" }, { "size":"1 x 100 mL", "name":"IgM Sample Diluent", "number":"AB108748-CMP01", "productcode":"" }, { "size":"1 x 2 mL", "name":"Influenza Virus B IgM Negative Control", "number":"AB108748-CMP09", "productcode":"" }, { "size":"1 x 2 mL", "name":"Influenza Virus B IgM Positive Control", "number":"AB108748-CMP07", "productcode":"" }, { "size":"1 x 3 mL", "name":"Influenza Virus B IgM Cut off Control", "number":"AB108748-CMP08", "productcode":"" }, { "size":"1 x 50 mL", "name":"20X Washing Solution", "number":"AB108748-CMP05", "productcode":"" }, { "size":"1 x 15 mL", "name":"Stop Solution", "number":"AB108748-CMP11", "productcode":"" }, { "size":"1 x 15 mL", "name":"TMB Substrate Solution", "number":"AB108748-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Cover foil", "number":"AB108748-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Strip holder", "number":"AB108748-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Influenza virus B (IgM) Coated Microplate (12 x 8 wells)", "number":"AB108748-CMP10", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Influenza Virus B IgM is an immunoglobulin that plays a mechanical role in the early immune response against Influenza B virus. This antibody often identified by its alternate name IgM has a pentameric structure with a molecular mass of approximately 900 kDa. Unlike other antibodies IgM is predominantly expressed in the blood and lymphatic fluid. It helps in recognizing antigens and initiating immune defense mechanisms.
Biological function summary

Antibodies like IgM engage with the immune response by binding to the viral antigens and initiating complement activation. IgM is not part of a larger antibody complex but functions independently to activate the innate immune response. This process assists the body's defense system to identify and neutralize the Influenza B virus efficiently. IgM plays a critical role as the first line of defense before other antibodies such as IgG come into the action during viral infection.

Pathways

Influenza B IgM plays a significant role in the complement activation pathway and the classical pathway. Through these pathways IgM facilitates the activation of complement proteins that mediate the destruction of the virus. This function links IgM to important proteins such as C1q which is essential for the initiation of the classical complement pathway. Such pathways are necessary for the recognition and elimination of pathogens from the host.

Influenza B IgM levels rise significantly during an Influenza B virus infection playing a role in clearing the virus from the body. Health professionals utilize IgM levels to determine early-stage infection. While not directly relating to chronic diseases IgM level evaluation helps in distinguishing acute viral conditions from other non-viral disorders. It interfaces with proteins involved in immune reactions solidifying its importance in the diagnosis and understanding of immune response to respiratory infections.
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Product protocols

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Target data

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in nutrition 10:1116634 PubMed37168053

2023

Healthy adults supplemented with a nutraceutical formulation containing gel, rosemary and enhances the effect of influenza vaccination in a randomized, triple-blind, placebo-controlled trial.

Applications

Unspecified application

Species

Unspecified reactive species

Erin D Lewis,David C Crowley,Najla Guthrie,Malkanthi Evans

Vaccines 10: PubMed35891309

2022

Adults with Trisomy 21 Have Differential Antibody Responses to Influenza A.

Applications

Unspecified application

Species

Unspecified reactive species

Stephanie James,Robert C Haight,Cassandra Hanna,Lindsey Furton
View all publications
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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