Human INHBE ELISA Kit (Inhibin beta E) (ab324257) is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of INHbE in human serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids.
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Key facts
- Sample types
- Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell culture supernatant, Other biological fluids
- Reactive species
- Human
- Range
- 4.8 - 3000 pg/mL
- Assay time
- 2h
- Sensitivity
- = 1.7 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application cELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins.
Alternative names
Inhibin beta E chain, Activin beta-E chain, INHBE
What's included?
Human INHBE ELISA Kit (Inhibin beta E) (ab324257) is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of INHbE in human serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids.
Key facts
- Sample types
- Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell culture supernatant, Other biological fluids
- Reactive species
- Human
- Range
- 4.8 - 3000 pg/mL
- Assay time
- 2h
- Sensitivity
- = 1.7 pg/mL
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Sample | n 3 | C.V. < 10 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Sample | n 8 | C.V. < 12 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 97 | Range 86 - 105 % |
Sample type EDTA Plasma | Average % = 94 | Range 90 - 101 % |
Sample type Heparin Plasma | Average % = 86 | Range 82 - 93 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Please refer to protocols
Notes
Human INHBE ELISA Kit (Inhibin beta E) (ab324257) is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of INHbE in human serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids.
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1 product image
Sandwich ELISA - Human INHBE ELISA Kit (Inhibin beta E) (ab324257)
In order to make the calculation easier, we plot the O.D. value of the standard (X-axis) against the log of concentration of the standard (Y-axis), although concentration is the independent variable and O.D. value is the dependent variable. The O.D. values of the standard curve may vary according to the conditions of assay performance (e.g. operator, pipetting technique, washing technique or temperature effects). Typical standard curve below is provided for reference only.
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Product protocols
- Visit the general protocols
- Visit troubleshooting
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