AB324257
Human INHBE ELISA Kit (Inhibin beta E)
Be the first to review this product! Submit a review
|
(0 Publication)
Human INHBE ELISA Kit (Inhibin beta E) (ab324257) is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of INHbE in human serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids.
View Alternative Names
Inhibin beta E chain, Activin beta-E chain, INHBE
1 Images
- sELISA
Supplier Data
Sandwich ELISA - Human INHBE ELISA Kit (Inhibin beta E) (AB324257)
In order to make the calculation easier, we plot the O.D. value of the standard (X-axis) against the log of concentration of the standard (Y-axis), although concentration is the independent variable and O.D. value is the dependent variable. The O.D. values of the standard curve may vary according to the conditions of assay performance (e.g. operator, pipetting technique, washing technique or temperature effects). Typical standard curve below is provided for reference only.
Reactivity data
{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "cELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
Product details
Human INHBE ELISA Kit (Inhibin beta E) (ab324257) is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of INHbE in human serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids.
Precision
[ { "reproducibilityType": "Intra", "sample": "Sample", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Inter", "sample": "Sample", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" } ]
Recovery
[ { "sample": "Serum", "range": "86 - 105 %", "average": "= 97" }, { "sample": "EDTA Plasma", "range": "90 - 101 %", "average": "= 94" }, { "sample": "Heparin Plasma", "range": "82 - 93 %", "average": "= 86" } ]
What's included?
{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab324257-1x96Tests", "assetComponentDetails": [ { "size":"1 x 20 mL", "name":"Wash Buffer", "number":"AB324257-CMP10" }, { "size":"1 x 9 mL", "name":"TMB Substrate", "number":"AB324257-CMP08" }, { "size":"1 x 6 mL", "name":"Stop Solution", "number":"AB324257-CMP09" }, { "size":"4 x 1 Unit", "name":"Plate Sealer", "number":"AB324257-CMP11" }, { "size":"2 x 1 Vial", "name":"Human INHBE standard", "number":"AB324257-CMP02" }, { "size":"1 x 1 Unit", "name":"Human INHBE precoated plate", "number":"AB324257-CMP01" }, { "size":"1 x 120 µL", "name":"Human INHBE detection reagent B", "number":"AB324257-CMP06" }, { "size":"1 x 120 µL", "name":"Human INHBE detection reagent A", "number":"AB324257-CMP04" }, { "size":"1 x 20 mL", "name":"Human INHBE Standard Diluent", "number":"AB324257-CMP03" }, { "size":"1 x 12 mL", "name":"Human INHBE Assay diluent B", "number":"AB324257-CMP07" }, { "size":"1 x 12 mL", "name":"Human INHBE Assay diluent A", "number":"AB324257-CMP05" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab324257-1x96Tests", "assetComponentDetails": [ { "size":"1 x 20 mL", "name":"Wash Buffer", "number":"AB324257-CMP10", "productcode":"" }, { "size":"1 x 9 mL", "name":"TMB Substrate", "number":"AB324257-CMP08", "productcode":"" }, { "size":"1 x 6 mL", "name":"Stop Solution", "number":"AB324257-CMP09", "productcode":"" }, { "size":"4 x 1 Unit", "name":"Plate Sealer", "number":"AB324257-CMP11", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human INHBE standard", "number":"AB324257-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human INHBE precoated plate", "number":"AB324257-CMP01", "productcode":"" }, { "size":"1 x 120 µL", "name":"Human INHBE detection reagent B", "number":"AB324257-CMP06", "productcode":"" }, { "size":"1 x 120 µL", "name":"Human INHBE detection reagent A", "number":"AB324257-CMP04", "productcode":"" }, { "size":"1 x 20 mL", "name":"Human INHBE Standard Diluent", "number":"AB324257-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"Human INHBE Assay diluent B", "number":"AB324257-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"Human INHBE Assay diluent A", "number":"AB324257-CMP05", "productcode":"" } ] } } }
Properties and storage information
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
Please refer to protocols
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
- Download websiteProtocolBooklet|en
Target data
Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins.. Activin E is a homodimer of INHBE secreted by the liver that plays a crucial role in regulating metabolic homeostasis particularly in lipid metabolism and energy homeostasis. Plays a central role in the regulation of adipose tissue lipolysis by preventing the influx of fatty acids from adipose tissue into the liver. Mechanistically, signals via ACVR1C to activate SMAD2/3 signaling, suppressing PPARG target genes in adipose tissue, thereby reducing liver lipid content and improving glycemic control (PubMed : 38533769). Induces beige adipocyte formation and thermogenesis in response to cold exposure (By similarity).
Product promise
We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com