AB253217

Human iNOS ELISA Kit

Recombinant
SimpleStep
What is this?

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(9 Publications)

Human iNOS ELISA Kit is a single-wash 90-min Simplestep used to quantify Human iNOS with a sensitivity of 18 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 5 citations

View Alternative Names

NOS2A, NOS2, Hepatocyte NOS, Inducible NO synthase, NOS type II, Peptidyl-cysteine S-nitrosylase NOS2, HEP-NOS, Inducible NOS, iNOS

2 Images

Sandwich ELISA - Human iNOS ELISA Kit (AB253217)

sELISA

Unknown

Sandwich ELISA - Human iNOS ELISA Kit (AB253217)

Interpolated concentrations of native iNOS in Caco-2 cell extract samples based on an 800 μg/mL extract load.

The concentrations of iNOS were measured in duplicate and interpolated from the iNOS standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean iNOS concentration was determined to be 613.7 pg/mL in Caco-2 cell extract samples.

sELISA

Supplier Data

Sandwich ELISA - Human iNOS ELISA Kit (AB253217)

Example of human iNOS standard curve in 1X Cell Extraction Buffer PTR.

The iNOS standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 18 pg/mL

Range

46.88 - 3000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human iNOS ELISA Kit (ab253217) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of iNOS protein in cell lysate. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human iNOS with 18 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

iNOS, also known as NOS2, catalyzes the production of nitric oxide from L-arginine, which is used in various cell signaling events. iNOS is an inducible member of the NOS family and is upregulated as a host-defense mechanism during proinflammatory cytokine activity seen with tumorigenesis and parasitic infection. iNOS activity is a key cause of septic shock.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.3" } ]

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Recovery

[ { "sample": "Cell Lysate", "range": null, "average": "= 95" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab253217-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB253217-CMP07", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB253217-CMP14", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB253217-CMP13", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB253217-CMP12", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human iNOS Capture Antibody", "number":"AB253217-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human iNOS Lyophilized Recombinant Protein", "number":"AB253217-CMP05", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB253217-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB253217-CMP06", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human iNOS Detector Antibody", "number":"AB253217-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB253217-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB253217-CMP08", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB253217-CMP10", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Inducible Nitric Oxide Synthase commonly referred to as iNOS or NOS2 is an important enzyme in the production of nitric oxide (NO) from L-arginine. This enzyme has a molecular weight of approximately 130 kDa and is expressed in various cell types including macrophages and hepatocytes. iNOS becomes active in response to inflammatory stimuli and can produce large amounts of NO for extended periods. Western blotting and iNOS ELISA kits are frequently used methods to detect and quantify the presence and activity of iNOS in different biological samples.

Biological function summary

INOS plays a significant role in the immune system by participating in the defense against pathogens. The enzyme is part of a molecular complex that generates NO which acts as a signaling molecule and has antimicrobial properties. iNOS activity is induced during immune responses and contributes to the elimination of bacteria and viruses through the production of reactive nitrogen species. Its expression is tightly regulated by cytokines and other signaling molecules that modulate the extent of its action.

Pathways

INOS integrates into the nitric oxide signaling pathway and is closely associated with inflammatory pathways. NO serves as a messenger within the nitric oxide signaling pathway facilitating processes like vasodilation and neurotransmission. iNOS works alongside other proteins such as cytokines like interferon-gamma that elevate its activity during inflammation and immune responses. The regulation of iNOS expression and activity plays an essential role in maintaining immune homeostasis and ensuring proper response to infections.

INOS is linked to conditions characterized by excessive inflammation such as rheumatoid arthritis and sepsis. In these diseases overproduction of nitric oxide by iNOS can lead to tissue damage and exacerbate symptoms. Additionally iNOS is associated with the protein TNF-alpha as its expression is often driven by inflammatory mediators during disease states. Understanding the role of iNOS in such conditions provides insights into potential therapeutic targets for managing inflammatory diseases.

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Product protocols

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Target data

Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body (PubMed : 7504305, PubMed : 7531687, PubMed : 7544004, PubMed : 7682706). In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such PTGS2/COX2 (By similarity). As component of the iNOS-S100A8/9 transnitrosylase complex involved in the selective inflammatory stimulus-dependent S-nitrosylation of GAPDH on 'Cys-247' implicated in regulation of the GAIT complex activity and probably multiple targets including ANXA5, EZR, MSN and VIM (PubMed : 25417112). Involved in inflammation, enhances the synthesis of pro-inflammatory mediators such as IL6 and IL8 (PubMed : 19688109).

See full target information NOS2

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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 24: PubMed37445909

2023

Evaluation of the Antioxidant and Antiangiogenic Activity of a Pomegranate Extract in BPH-1 Prostate Epithelial Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Valeria Consoli,Ilaria Burò,Maria Gulisano,Angela Castellano,Agata Grazia D'Amico,Velia D'Agata,Luca Vanella,Valeria Sorrenti

Experimental and therapeutic medicine 25:169 PubMed37006880

2023

IGFBP‑rP1 affects the proliferation, apoptosis and macrophage polarization of endometrial cancer cells by regulating the PI3K/AKT pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Juan Gao,Shiqi Suo,Jingxia Li,Chunxia Wang,Ranran Deng,Yuxia Hu,Chunzheng Zhang

Oncoimmunology 12:2152635 PubMed36605619

2023

Overcoming the resistance of hepatocellular carcinoma to PD-1/PD-L1 inhibitor and the resultant immunosuppression by CD38 siRNA-loaded extracellular vesicles.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Deng,Hui Ke

Medical oncology (Northwood, London, England) 39:214 PubMed36175598

2022

SPLUNC1 regulates LPS-induced progression of nasopharyngeal carcinoma and proliferation of myeloid-derived suppressor cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ling Tang,Ling Peng,Huai Liu,Tengfei Xiao,Wangning Gu,Hongmin Yang,Hui Wang,Pan Chen

Immunity, inflammation and disease 10:218-224 PubMed34847295

2021

LncRNA CCAT1 is overexpressed in tuberculosis patients and predicts their survival.

Applications

Unspecified application

Species

Unspecified reactive species

Taosheng Ye,Jiaohong Zhang,Xuan Zeng,Yuxiang Xu,Jinpei Li

Experimental and therapeutic medicine 22:1458 PubMed34737798

2021

Downregulation of microRNA-185 expression in diabetic patients increases the expression of NOS2 and results in vascular injury.

Applications

Unspecified application

Species

Unspecified reactive species

Yanyan Zhao,Liyan Guo,Jiajia Tian,Huafu Wang

BMC cardiovascular disorders 21:51 PubMed33526034

2021

CircTM7SF3 contributes to oxidized low-density lipoprotein-induced apoptosis, inflammation and oxidative stress through targeting miR-206/ASPH axis in atherosclerosis cell model in vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaojuan Wang,Ming Bai

Nature communications 11:6339 PubMed33311482

2020

Ferroptotic damage promotes pancreatic tumorigenesis through a TMEM173/STING-dependent DNA sensor pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Enyong Dai,Leng Han,Jiao Liu,Yangchun Xie,Herbert J Zeh,Rui Kang,Lulu Bai,Daolin Tang

Communications biology 3:604 PubMed33097805

2020

Lung gene expression signatures suggest pathogenic links and molecular markers for pulmonary tuberculosis, adenocarcinoma and sarcoidosis.

Applications

Unspecified application

Species

Unspecified reactive species

Qiyao Chai,Zhe Lu,Zhidong Liu,Yanzhao Zhong,Fuzhen Zhang,Changgen Qiu,Bingxi Li,Jing Wang,Lingqiang Zhang,Yu Pang,Cui Hua Liu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Human iNOS ELISA Kit

Sandwich ELISA - Human iNOS ELISA Kit (AB253217)

Sandwich ELISA - Human iNOS ELISA Kit (AB253217)

Key facts

Detection method

Sample types

Reacts with

Assay type

Results type

Sensitivity

Range

Assay time

Assay Platform

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Assay Kits

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (9)

Product promise