The Human insulin ELISA kit (colorimetric) is a sandwich ELISA designed to quantify insulin with a sensitivity of <4 µIU/mL.
This Human insulin ELISA kit offers:
- High sensitivity – detects insulin as low as <4 µIU/mL
- Wide dynamic range – quantifies 4.69–300 µIU/mL
- Compatible with multiple sample types – works with serum, plasma, and cell culture supernatants
- Cited in 41 publications
Colorimetric
Plasma, Cell culture supernatant, Serum
Sandwich (quantitative)
Human
4.69 - 300 µlU/mL
< 4 µlU/mL
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Select an associated product type
Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
Insulin, INS
The Human insulin ELISA kit (colorimetric) is a sandwich ELISA designed to quantify insulin with a sensitivity of <4 µIU/mL.
This Human insulin ELISA kit offers:
- High sensitivity – detects insulin as low as <4 µIU/mL
- Wide dynamic range – quantifies 4.69–300 µIU/mL
- Compatible with multiple sample types – works with serum, plasma, and cell culture supernatants
- Cited in 41 publications
Colorimetric
Plasma, Cell culture supernatant, Serum
Sandwich (quantitative)
Human
4.69 - 300 µlU/mL
Microplate
< 4 µlU/mL
89%
Sample type | Average % | Range |
---|---|---|
Sample type Cell culture supernatant | Average % = 76.16 | Range 68 - 88 % |
Sample type Serum | Average % = 91.4 | Range 83 - 102 % |
Sample type Plasma | Average % = 99.03 | Range 73 - 128 % |
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Abcam's Human Insulin ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Insulin and Proinsulin in serum, plasma, cell culture supernatants.
This assay employs an antibody specific for Human Insulin coated on a 96-well plate. Standards and samples are pipetted into the wells and Insulin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Insulin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Insulin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
This kit detects both insulin and proinsulin.
Get higher sensitivity in only 90 minutes with Human Insulin ELISA Kit (Human Insulin ELISA Kit ab278123) from our SimpleStep ELISA® range.
Optimization may be required with urine samples.
This supplementary information is collated from multiple sources and compiled automatically.
Insulin a peptide hormone plays an essential mechanical role in carbohydrate and fat metabolism. Insulin alternatively known as bovine insulin in some research contexts has a molecular mass of around 5800 Da. Beta cells in the pancreas express insulin where it gets secreted directly into the bloodstream. The hormone regulates glucose levels by facilitating the uptake of glucose into cells such as muscle and adipose tissues.
Insulin influences several key metabolic processes by binding to its receptor a component of the insulin receptor substrate family. This triggers a cascade of events within cells promoting the uptake of glucose and converting it into energy. Insulin operates as a singular entity but plays a critical role in forming a complex with its receptor. This interaction triggers downstream effects that alter the activity of enzymes and transcription factors involved in glucose and lipid metabolism.
Insulin is critical for the regulation of the PI3K-AKT signaling pathway and the MAPK pathway. These pathways modulate processes such as cell growth proliferation and survival. Insulin interacts closely with other proteins in these pathways such as the insulin receptor substrate proteins and influences downstream proteins like AKT kinase which has a significant role in mediating the metabolic effects initiated by insulin binding.
Insulin is most notably linked to diabetes mellitus characterized by insufficient insulin production or action. This deficiency disrupts normal glucose homeostasis leading to hyperglycemia. Other associated proteins include glucagon which counteracts insulin's role in lowering blood glucose levels and IRS-1 an important element through which insulin signaling is transduced. Researchers utilize mouse insulin ELISA and insulin ELISA kits to better understand these relationships and develop treatments for insulin-related disorders.
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Terms & Conditions.
Human Insulin measured in cell culture supernatants showing quantity (μIU) per mL of tested sample. Samples diluted 1-2 fold.
Human Insulin measured in biological fluids showing quantity (μIU) per mL of tested sample. Samples diluted 1-6 fold.
Human Insulin standard curve: mean of duplicates (+/- SD), performed in Diluent A
Representative Standard Curve using ab100578.
Representative Standard Curve using ab100578.
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