Human Insulin ELISA Kit

Product details

Human Insulin ELISA Kit ab100578 is a sandwich ELISA to measure human Insulin (INS) and Proinsulin in serum, plasma, and cell culture supernatant with a sensitivity of <4 µIU/mL.

How the assay works

This assay employs an antibody specific for Human Insulin coated on a 96-well plate. Standards and samples are pipetted into the wells and Insulin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Insulin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated Streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Insulin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Assay specificity

This kit recognizes Insulin and Proinsulin.
Cross reactivity: This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human BDNF, BLC, ENA-78, IL-1α, IL-1β, IL-2, IL-3, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, G-CSF, GM-CSF, IFN-γ, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1α, MIP-1β, MIP-1δ, PARC, PDGF, RANTES, SCF, TARC, TGF-β, TIMP-1, TIMP-2, TNF-α, TNF-β, TPO, VEGF.

Human Insulin ELISA Kit ab100578 protocol summary

1. Add samples and standards to wells. Incubate at room temperature.
2. Wash each well and add biotinylated antibody. Incubate at room temperature.
3. Wash each well and add Streptavidin Solution. Incubate at room temperature.
4. Add TMB Solution to each well. Incubate at room temperature.
5. Add Stop Solution to each well. Read immediately.

How other researchers are using Human Insulin ELISA Kit ab100578

Human Insulin ELISA Kit ab100578 has been used in publications to study the effects of NRG1 in children with obesity ⁽¹⁾ and therapeutic effects of mesenchymal stem cells in diabetes ⁽²⁾.

References:

⁽¹⁾Johnny D Figueroa et al. 2022 PMID: 35220393,
⁽²⁾Yongsung Hwang et al. 2023 PMID: 37644502

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- Human Insulin ELISA Kit ab278123

Related and recommended products

Human Insulin ELISA Kit ab100578 is commonly used to study diabetes and insulin disorders.

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- Human IgG ELISA Kit ab195215
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