AB278123

Human Insulin ELISA Kit

Recombinant
SimpleStep
What is this?

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(6 Publications)

Human Insulin ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Insulin with a sensitivity of 7.13 pmol/L. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Insulin, INS

5 Images

ELISA - Human Insulin ELISA Kit (AB278123)

ELISA

Supplier Data

ELISA - Human Insulin ELISA Kit (AB278123)

sELISA

Unknown

Sandwich ELISA - Human Insulin ELISA Kit (AB278123)

Serum from ten individual healthy human male donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Donors 1, 2, 3, 8, and 10 interpolated below the standard curve, thus are not determined (ND). The mean Insulin concentration was determined to be 269.30 pmol/L with a range of ND – 1,949.53 pmol/L. Health history and dietary status of donors were unknown.

sELISA

Unknown

Sandwich ELISA - Human Insulin ELISA Kit (AB278123)

Serum from three individual human male donors with type 2 diabetes mellitus was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Insulin concentration was determined to be 206.71 pmol/L with a range of 109.17 – 359.67 pmol/L.

sELISA

Unknown

Sandwich ELISA - Human Insulin ELISA Kit (AB278123)

Interpolated concentrations of native Insulin in human serum and plasma (EDTA) samples.

The concentrations of Insulin were measured in duplicates, interpolated from the Insulin standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 95% and plasma (EDTA) 95%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Insulin concentration was determined to be 95.15 pmol/L in serum and 240.40 pmol/L in plasma (EDTA).

sELISA

Supplier Data

Sandwich ELISA - Human Insulin ELISA Kit (AB278123)

Example of human Insulin standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 7.13 pmol/L

Range

26.56 - 425 pmol/L

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human Insulin ELISA Kit ab278123 is a rapid single-wash 90-min Sandwich ELISA to measure Human Insulin in EDTA plasma, heparin plasma, serum. This SimpleStep sensitivity is 7.13 pmoL/L.

How the assay works

Human Insulin SimpleStep ELISA^{^®}employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^{^®} plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA^{^®} protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA^® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human Insulin ELISA Kit ab278123 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-Insulin antibody [EPR22681-20] - BSA and Azide free (Capture) ab281545
- Anti-Insulin antibody [EPR22631-15] - BSA and Azide free (Detector) ab281545

Insulin is a highly conserved, secreted hormone essential for glucose metabolism. Produced by pancreatic beta cells, proinsulin is proteolyzed into an A and a B chain, which form a 6 kDa mature protein. Basal levels of insulin are continuously delivered into the bloodstream, and additional levels are secreted proportional to food ingestion. Insulin secretion is highly regulated, and dysregulation of insulin production or sensitivity results in Type 1 diabetes mellitus or Type 2 diabetes mellitus, respectively.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "11.8" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "9.4" } ]

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Recovery

[ { "sample": "Serum", "range": "94 - 98 %", "average": "= 96" }, { "sample": "EDTA Plasma", "range": "95 - 103 %", "average": "= 99" }, { "sample": "Heparin Plasma", "range": "80 - 97 %", "average": "= 89" } ]

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What's included?

{ "values": { "10x96Tests": { "sellingSize": "10 x 96 Tests", "publicAssetCode":"ab278123-10x96Tests", "assetComponentDetails": [ { "size":"10 x 600 µL", "name":"10X Human Insulin Capture Antibody", "number":"AB278123-CMP07" }, { "size":"2 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB278123-CMP11" }, { "size":"1 x 200 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB278123-CMP10" }, { "size":"10 x 1 Unit", "name":"Plate Seals", "number":"AB278123-CMP09" }, { "size":"10 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB278123-CMP12" }, { "size":"10 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB278123-CMP04" }, { "size":"20 x 1 Vial", "name":"Human Insulin Lyophilized Recombinant Protein", "number":"AB278123-CMP03" }, { "size":"1 x 120 mL", "name":"Stop Solution", "number":"AB278123-CMP05" }, { "size":"1 x 120 mL", "name":"TMB Development Solution", "number":"AB278123-CMP06" }, { "size":"10 x 600 µL", "name":"10X Human Insulin Detector Antibody", "number":"AB278123-CMP08" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab278123-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB278123-CMP05", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Insulin Capture Antibody", "number":"AB278123-CMP07", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB278123-CMP12", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB278123-CMP11", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB278123-CMP10", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Insulin Lyophilized Recombinant Protein", "number":"AB278123-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB278123-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB278123-CMP09", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB278123-CMP06", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Insulin Detector Antibody", "number":"AB278123-CMP08", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Insulin a peptide hormone plays an essential mechanical role in carbohydrate and fat metabolism. Insulin alternatively known as bovine insulin in some research contexts has a molecular mass of around 5800 Da. Beta cells in the pancreas express insulin where it gets secreted directly into the bloodstream. The hormone regulates glucose levels by facilitating the uptake of glucose into cells such as muscle and adipose tissues.

Biological function summary

Insulin influences several key metabolic processes by binding to its receptor a component of the insulin receptor substrate family. This triggers a cascade of events within cells promoting the uptake of glucose and converting it into energy. Insulin operates as a singular entity but plays a critical role in forming a complex with its receptor. This interaction triggers downstream effects that alter the activity of enzymes and transcription factors involved in glucose and lipid metabolism.

Pathways

Insulin is critical for the regulation of the PI3K-AKT signaling pathway and the MAPK pathway. These pathways modulate processes such as cell growth proliferation and survival. Insulin interacts closely with other proteins in these pathways such as the insulin receptor substrate proteins and influences downstream proteins like AKT kinase which has a significant role in mediating the metabolic effects initiated by insulin binding.

Insulin is most notably linked to diabetes mellitus characterized by insufficient insulin production or action. This deficiency disrupts normal glucose homeostasis leading to hyperglycemia. Other associated proteins include glucagon which counteracts insulin's role in lowering blood glucose levels and IRS-1 an important element through which insulin signaling is transduced. Researchers utilize mouse insulin ELISA and insulin ELISA kits to better understand these relationships and develop treatments for insulin-related disorders.

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Product protocols

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Target data

Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.

See full target information INS

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Clinical and translational science 18:e70120 PubMed39943692

2025

The Pharmacokinetic Interaction Between Metformin and the Natural Product Goldenseal Is Metformin Dose-Dependent: A Three-Arm Crossover Study in Adults With Type 2 Diabetes.

Applications

Unspecified application

Species

Unspecified reactive species

James T Nguyen,Christopher M Arian,Rakshit S Tanna,Maxey G Cherel,Matthew E Layton,John R White,Kenneth E Thummel,Mary F Paine

Clinical infectious diseases : an official publication of the Infectious Diseases Society of America 79:727-733 PubMed38531012

2024

Lower Insulin Sensitivity Through 36 Months of Life With in Utero HIV and Antiretroviral Exposure in Botswana: Results From the Tshilo Dikotla Study.

Applications

Unspecified application

Species

Unspecified reactive species

Jennifer Jao,Lauren B Bonner,Katrina Dobinda,Kathleen M Powis,Shan Sun,Justine Legbedze,Keolebogile N Mmasa,Joseph Makhema,Mompati Mmalane,Samuel Kgole,Gosego Masasa,Sikhulile Moyo,Mariana Gerschenson,Terence Mohammed,Elaine J Abrams,Irwin J Kurland,Mitchell E Geffner

iScience 26:105723 PubMed36590169

2023

Assessment of stromal SCD-induced drug resistance of PDAC using 3D-printed zPDX model chips.

Applications

Unspecified application

Species

Unspecified reactive species

Chuntao Wu,Beiyuan Hu,Lei Wang,Xia Wu,Haitao Gu,Hanguang Dong,Jiuliang Yan,Zihao Qi,Qi Zhang,Huan Chen,Bo Yu,Sheng Hu,Yu Qian,Shuang Dong,Qiang Li,Xu Wang,Jiang Long

The Journal of infectious diseases 226:2002-2009 PubMed36240387

2022

Lower Insulin Sensitivity in Newborns With In Utero HIV and Antiretroviral Exposure Who Are Uninfected in Botswana.

Applications

Unspecified application

Species

Unspecified reactive species

Jennifer Jao,Shan Sun,Lauren B Bonner,Justine Legbedze,Keolebogile N Mmasa,Joseph Makhema,Mompati Mmalane,Samuel Kgole,Gosego Masasa,Sikhulile Moyo,Mariana Gerschenson,Terence Mohammed,Elaine J Abrams,Irwin J Kurland,Mitchell E Geffner,Kathleen M Powis

International journal of molecular sciences 23: PubMed35805883

2022

Therapeutic Effects of Insulin-Producing Human Umbilical Cord-Derived Mesenchymal Stem Cells in a Type 1 Diabetes Mouse Model.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Mi Park,Chang Mo Yang,Hee Yeon Cho

The Journal of biological chemistry 298:102128 PubMed35700823

2022

A multi-omics analysis reveals that the lysine deacetylase ABHD14B influences glucose metabolism in mammals.

Applications

Unspecified application

Species

Unspecified reactive species

Abinaya Rajendran,Amarendranath Soory,Neha Khandelwal,Girish Ratnaparkhi,Siddhesh S Kamat

View all publications

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human Insulin ELISA Kit

ELISA - Human Insulin ELISA Kit (AB278123)

Sandwich ELISA - Human Insulin ELISA Kit (AB278123)

Sandwich ELISA - Human Insulin ELISA Kit (AB278123)

Sandwich ELISA - Human Insulin ELISA Kit (AB278123)

Sandwich ELISA - Human Insulin ELISA Kit (AB278123)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

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