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AB278125

Human Insulin ELISA Kit, fluorescent

  • CatchPoint SimpleStep ELISA
  • Recombinant
  • SimpleStep
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Human Insulin ELISA Kit, fluorescent is a single-wash 90-min Simplestep used to quantify Human Insulin with a sensitivity of 8.04 pmol/L. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Fluorescent Sandwich ELISA - 530/570/590 nm readout : works on any standard plate reader

View Alternative Names

Insulin, INS

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Sandwich ELISA - Human Insulin ELISA Kit, fluorescent (AB278125)
  • sELISA

Supplier Data

Sandwich ELISA - Human Insulin ELISA Kit, fluorescent (AB278125)

Example of human Insulin standard curve in Sample Diluent NS.

The Insulin standard curve was prepared as described in Section 10. Raw data generated on SpectraMax M4 Multi-Mode Microplate Reader is shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Fluorescent

Sample types

Heparin Plasma, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 8.04 pmol/L

Range

13.28 - 425 pmol/L

Assay time

1h 30m

Assay Platform

Microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Insulin in vitro CatchPoint® SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Insulin protein in human serum, plasma heparin, and plasma EDTA samples.

This CatchPoint SimpleStep ELISA kit has been optimized for Molecular Devices Microplate Readers. Click here for a list of recommended Microplate Readers.
If using a Molecular Devices' plate reader supported by SoftMax® Pro software, a preconfigured protocol for these CatchPoint SimpleStep ELISA Kits is available with all the protocol and analysis settings at www.softmaxpro.org

The CatchPoint SimpleStep ELISA employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. CatchPoint HRP Development Solution containing the Stoplight Red Substrate is added. During incubation, the substrate is catalyzed by HRP generating a fluorescent product. Signal is generated proportionally to the amount of bound analyte and the intensity is measured in a fluorescence plater reader at 530/570/590 nm Excitation/Cutoff/Emission.

Insulin is a highly conserved, secreted hormone essential for glucose metabolism. Produced by pancreatic beta cells, proinsulin is proteolyzed into an A and a B chain, which form a 6 kDa mature protein. Basal levels of insulin are continuously delivered into the bloodstream, and additional levels are secreted proportional to food ingestion. Insulin secretion is highly regulated, and dysregulation of insulin production or sensitivity results in Type 1 diabetes mellitus or Type 2 diabetes mellitus, respectively.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "11.8" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "9.4" } ]
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Recovery

[ { "sample": "Serum", "range": "94 - 98 %", "average": "= 96" }, { "sample": "EDTA Plasma", "range": "95 - 103 %", "average": "= 99" }, { "sample": "Heparin Plasma", "range": "80 - 97 %", "average": "= 89" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab278125-1x96Tests", "assetComponentDetails": [ { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB278125-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB278125-CMP12", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB278125-CMP10", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Insulin Lyophilized Recombinant Protein", "number":"AB278125-CMP04", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Insulin Capture Antibody", "number":"AB278125-CMP08", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPI - HAMA Blocker (ab193969)", "number":"AB278125-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated Black 96-Well Microplate", "number":"AB278125-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stoplight Red Substrate Buffer", "number":"AB278125-CMP06", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Insulin Detector Antibody", "number":"AB278125-CMP09", "productcode":"" }, { "size":"1 x 120 µL", "name":"100X Stoplight Red Substrate", "number":"AB278125-CMP01", "productcode":"" }, { "size":"1 x 50 µL", "name":"500X Hydrogen Peroxide (H2O2, 3%)", "number":"AB278125-CMP07", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Insulin a peptide hormone plays an essential mechanical role in carbohydrate and fat metabolism. Insulin alternatively known as bovine insulin in some research contexts has a molecular mass of around 5800 Da. Beta cells in the pancreas express insulin where it gets secreted directly into the bloodstream. The hormone regulates glucose levels by facilitating the uptake of glucose into cells such as muscle and adipose tissues.
Biological function summary

Insulin influences several key metabolic processes by binding to its receptor a component of the insulin receptor substrate family. This triggers a cascade of events within cells promoting the uptake of glucose and converting it into energy. Insulin operates as a singular entity but plays a critical role in forming a complex with its receptor. This interaction triggers downstream effects that alter the activity of enzymes and transcription factors involved in glucose and lipid metabolism.

Pathways

Insulin is critical for the regulation of the PI3K-AKT signaling pathway and the MAPK pathway. These pathways modulate processes such as cell growth proliferation and survival. Insulin interacts closely with other proteins in these pathways such as the insulin receptor substrate proteins and influences downstream proteins like AKT kinase which has a significant role in mediating the metabolic effects initiated by insulin binding.

Insulin is most notably linked to diabetes mellitus characterized by insufficient insulin production or action. This deficiency disrupts normal glucose homeostasis leading to hyperglycemia. Other associated proteins include glucagon which counteracts insulin's role in lowering blood glucose levels and IRS-1 an important element through which insulin signaling is transduced. Researchers utilize mouse insulin ELISA and insulin ELISA kits to better understand these relationships and develop treatments for insulin-related disorders.
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Product protocols

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Target data

Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
See full target information INS
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websiteProtocolBooklet
en
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Product promise

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