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Human Integrin alpha 2 ELISA kit is a Sandwich (quantitative) ELISA kit for the measurement of Human Integrin alpha 2 in Human in Serum, Plasma, Cell culture supernatant samples.

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Key facts

Detection method

Colorimetric

Sample types

Serum, Plasma, Cell culture supernatant

Assay type

Sandwich (quantitative)

Reactive species

Human

Range

0.102 - 25 ng/mL

Sensitivity

= 0.1 ng/mL

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Reactivity data

Application

sELISA

Reactivity

Reacts

Dilution info

-

Notes

-

Target data

Function

Integrin alpha-2/beta-1 is a receptor for laminin, collagen, collagen C-propeptides, fibronectin and E-cadherin. It recognizes the proline-hydroxylated sequence G-F-P-G-E-R in collagen. It is responsible for adhesion of platelets and other cells to collagens, modulation of collagen and collagenase gene expression, force generation and organization of newly synthesized extracellular matrix.(Microbial infection) Integrin ITGA2:ITGB1 acts as a receptor for Human rotavirus A.(Microbial infection) Integrin ITGA2:ITGB1 acts as a receptor for Human echoviruses 1 and 8.

Alternative names

What's included?

1 x 96 Tests
Components
200X HRP-Streptavidin
1 x 200 µL
20X Wash Buffer
1 x 25 mL
5X Assay Diluent (Item E2)
1 x 15 mL
Biotinylated Human ITGA2 Detection Antibody
2 x 1 Vial
Human ITGA2 Antibody-coated ELISA Plate
1 x 1 Unit
Lyophilized Human ITGA2 Protein Standard
2 x 1 Vial
Stop Solution; 0.2M Sulfuric Acid
1 x 8 mL
TMB One-Step Substrate Reagent
1 x 12 mL

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Human Integrin alpha 2 ELISA kit is a Sandwich (quantitative) ELISA kit for the measurement of Human Integrin alpha 2 in Human in Serum, Plasma, Cell culture supernatant samples.

Alternative names

Key facts

Detection method

Colorimetric

Sample types

Serum, Plasma, Cell culture supernatant

Assay type

Sandwich (quantitative)

Reactive species

Human

Range

0.102 - 25 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

Sensitivity

= 0.1 ng/mL

Recovery

Sample specific recovery

Sample type

Serum

Average %

= 120.6

Range

105 - 131 %

Sample type

Plasma

Average %

= 103

Range

84 - 120 %

Sample type

Cell culture media

Average %

= 116.7

Range

95 - 137 %

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

-20°C

Storage information

-20°C

Notes

Human Integrin alpha 2 ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Integrin alpha 2 in serum, plasma, and cell culture supernatants. This assay employs an antibody specific for human Integrin alpha 2 coated on a 96-well plate

Supplementary info

Biological function summary

Integrin alpha 2 plays a significant role in cellular processes like migration and proliferation. It forms a heterodimer complex by pairing with the beta 1-integrin subunit contributing to its adhesive and signaling functions. This association enables the integrin alpha 2 complex to bind specific extracellular matrix components such as collagen and laminin. Through these interactions it regulates processes like wound healing and tissue remodeling and affects how cells respond to mechanical stress.

Activity summary

Integrin alpha 2 also known as CD49b ITGA2 and A2 is a protein that functions as part of the integrin family. Integrins are transmembrane receptors that facilitate cell-extracellular matrix adhesion. Integrin alpha 2 has a molecular mass of approximately 130 kDa and is widely expressed in epithelial cells fibroblasts and platelets. This protein is important for mediating adhesion between cells and the extracellular matrix affecting cell signaling and behavior. Through this adhesion cells can communicate with their environment adjusting their functions accordingly.

Pathways

Integrin alpha 2 is involved in major signaling routes that influence cell fate and movement. Notably it participates in the PI3K/AKT and MAPK pathways which are important for regulating cell growth and survival. It interacts with signaling proteins like focal adhesion kinase (FAK) and Src family kinases linking extracellular matrix changes to intracellular responses. These pathways help in transmitting signals that coordinate the cell's structural and functional adjustments in response to its surroundings.

Associated diseases and disorders

Integrin alpha 2 has connections to cancer and fibrosis. In cancers its altered expression may contribute to tumor progression and metastasis often involving interactions with other integrins and matrix metalloproteinases that degrade extracellular components. In fibrosis excessive integrin alpha 2 activity can lead to abnormal tissue remodeling and scarring implicating its role alongside collagen-producing cells. Understanding these connections is vital for developing therapies targeting integrin-mediated pathways in these diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Sandwich ELISA - Human Integrin alpha 2 ELISA kit (ab309428), expandable thumbnail

    Sandwich ELISA - Human Integrin alpha 2 ELISA kit (ab309428)

    Example of standard curve using ab309428. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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