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AB309428

Human Integrin alpha 2 ELISA kit

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Human Integrin alpha 2 ELISA kit is a Sandwich ELISA for the measurement of Human Integrin alpha 2 in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

CD49b, CD49B, ITGA2, Integrin alpha-2, CD49 antigen-like family member B, Collagen receptor, Platelet membrane glycoprotein Ia, VLA-2 subunit alpha, GPIa

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Sandwich ELISA - Human Integrin alpha 2 ELISA kit (AB309428)
  • sELISA

Supplier Data

Sandwich ELISA - Human Integrin alpha 2 ELISA kit (AB309428)

Example of standard curve using ab309428. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Serum, Plasma, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.1 ng/mL

Range

0.102 - 25 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Complementary Products

Primary Antibodies

AB181548

Anti-Integrin alpha 2 antibody [EPR17338] - C-terminal

RabMAb|Recombinant|KO Validated|20ul selling size

Flow Cytometry (Intracellular) - Anti-Integrin alpha 2 antibody [EPR17338] - C-terminal (AB181548)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Integrin alpha 2 ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Integrin alpha 2 in serum, plasma, and cell culture supernatants. This assay employs an antibody specific for human Integrin alpha 2 coated on a 96-well plate
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Recovery

[ { "sample": "Serum", "range": "105 - 131 %", "average": "= 120.6" }, { "sample": "Plasma", "range": "84 - 120 %", "average": "= 103" }, { "sample": "Cell culture media", "range": "95 - 137 %", "average": "= 116.7" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab309428-1x96Tests", "assetComponentDetails": [ { "size":"1 x 15 mL", "name":"5X Assay Diluent (Item E2)", "number":"AB309428-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human ITGA2 Protein Standard", "number":"AB309428-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB309428-CMP07", "productcode":"" }, { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin", "number":"AB309428-CMP11", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human ITGA2 Detection Antibody", "number":"AB309428-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human ITGA2 Antibody-coated ELISA Plate", "number":"AB309428-CMP08", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB309428-CMP06", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB309428-CMP04", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Integrin alpha 2 also known as CD49b ITGA2 and A2 is a protein that functions as part of the integrin family. Integrins are transmembrane receptors that facilitate cell-extracellular matrix adhesion. Integrin alpha 2 has a molecular mass of approximately 130 kDa and is widely expressed in epithelial cells fibroblasts and platelets. This protein is important for mediating adhesion between cells and the extracellular matrix affecting cell signaling and behavior. Through this adhesion cells can communicate with their environment adjusting their functions accordingly.
Biological function summary

Integrin alpha 2 plays a significant role in cellular processes like migration and proliferation. It forms a heterodimer complex by pairing with the beta 1-integrin subunit contributing to its adhesive and signaling functions. This association enables the integrin alpha 2 complex to bind specific extracellular matrix components such as collagen and laminin. Through these interactions it regulates processes like wound healing and tissue remodeling and affects how cells respond to mechanical stress.

Pathways

Integrin alpha 2 is involved in major signaling routes that influence cell fate and movement. Notably it participates in the PI3K/AKT and MAPK pathways which are important for regulating cell growth and survival. It interacts with signaling proteins like focal adhesion kinase (FAK) and Src family kinases linking extracellular matrix changes to intracellular responses. These pathways help in transmitting signals that coordinate the cell's structural and functional adjustments in response to its surroundings.

Integrin alpha 2 has connections to cancer and fibrosis. In cancers its altered expression may contribute to tumor progression and metastasis often involving interactions with other integrins and matrix metalloproteinases that degrade extracellular components. In fibrosis excessive integrin alpha 2 activity can lead to abnormal tissue remodeling and scarring implicating its role alongside collagen-producing cells. Understanding these connections is vital for developing therapies targeting integrin-mediated pathways in these diseases.
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Product protocols

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Target data

Integrin alpha-2/beta-1 is a receptor for laminin, collagen, collagen C-propeptides, fibronectin and E-cadherin. It recognizes the proline-hydroxylated sequence G-F-P-G-E-R in collagen. It is responsible for adhesion of platelets and other cells to collagens, modulation of collagen and collagenase gene expression, force generation and organization of newly synthesized extracellular matrix.. (Microbial infection) Integrin ITGA2 : ITGB1 acts as a receptor for Human rotavirus A.. (Microbial infection) Integrin ITGA2 : ITGB1 acts as a receptor for Human echoviruses 1 and 8.
See full target information ITGA2
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websiteProtocolBooklet
en
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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