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AB323925

Human Integrin alpha 2 ELISA Kit

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Human Integrin alpha 2 ELISA Kit is a Sandwich ELISA for the measurement of Human Integrin alpha 2 in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

CD49b, CD49B, ITGA2, Integrin alpha-2, CD49 antigen-like family member B, Collagen receptor, Platelet membrane glycoprotein Ia, VLA-2 subunit alpha, GPIa

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Sandwich ELISA - Human Integrin alpha 2 ELISA Kit (AB323925)
  • sELISA

Supplier Data

Sandwich ELISA - Human Integrin alpha 2 ELISA Kit (AB323925)

Serum of ten individual healthy human female donors was measured in duplicate. Interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). The mean Integrin alpha 2 concentration was determined to be 75.12 ng/mL with a range of 34.54 – 103.82 ng/mL.

Sandwich ELISA - Human Integrin alpha 2 ELISA Kit (AB323925)
  • sELISA

Supplier Data

Sandwich ELISA - Human Integrin alpha 2 ELISA Kit (AB323925)

Interpolated concentration of native Integrin alpha 2 was measured in duplicate at different sample concentrations. Undiluted samples are as follows : serum 1 : 20, plasma (citrate) 1 : 25, plasma (EDTA) 1 : 67, and plasma (heparin) 1 : 33. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human Integrin alpha 2 ELISA Kit (AB323925)
  • sELISA

Supplier Data

Sandwich ELISA - Human Integrin alpha 2 ELISA Kit (AB323925)

Example of human Integrin alpha 2 standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Integrin alpha 2 ELISA Kit (AB323925)
  • sELISA

Supplier Data

Sandwich ELISA - Human Integrin alpha 2 ELISA Kit (AB323925)

The concentration of Integrin alpha 2 was measured in wild-type and knockout HeLa cell extracts based on a 100 µg/mL extract load in 1X Cell Extraction Buffer PTR (not provided with this kit, but available as ab193970). The mean concentration was determined to be 2,953.1 pg/mL in wild-type cell extract and undetectable in ITGA2 knockout cell extract. The wild-type and Integrin alpha 2 knockout cell extracts are available as ab257262.

Key facts

Detection method

Colorimetric

Sample types

Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 34.3 pg/mL

Range

112.5 - 7200 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Integrin alpha 2 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Integrin alpha 2 protein in Serum, Cit Plasma, EDTA Plasma, Hep Plasma, Cell culture supernatant. Quantitate Human Integrin alpha 2 with 34.3 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibodyanalyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

Singlewash protocol reduces assay time to 90 minutes or less.

High sensitivity, specificity and reproducibility from superior antibodies.

Fully validated in biological samples.

96wells plate breakable into 12 x 8 wells strips, available in 14pack (10 x 96well plates).

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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 8.3" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 4.4" } ]
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Recovery

[ { "sample": "Serum", "range": "94 - 96 %", "average": "= 95" }, { "sample": "Citrate plasma", "range": "94 - 125 %", "average": "= 109" }, { "sample": "EDTA Plasma", "range": "103 - 115 %", "average": "= 110" }, { "sample": "Heparin Plasma", "range": "106 - 126 %", "average": "= 115" }, { "sample": "Cell culture supernatant", "range": "83 - 102 %", "average": "= 94" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Integrin alpha-2/beta-1 is a receptor for laminin, collagen, collagen C-propeptides, fibronectin and E-cadherin. It recognizes the proline-hydroxylated sequence G-F-P-G-E-R in collagen. It is responsible for adhesion of platelets and other cells to collagens, modulation of collagen and collagenase gene expression, force generation and organization of newly synthesized extracellular matrix.. (Microbial infection) Integrin ITGA2 : ITGB1 acts as a receptor for Human rotavirus A.. (Microbial infection) Integrin ITGA2 : ITGB1 acts as a receptor for Human echoviruses 1 and 8.
See full target information ITGA2
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websiteProtocolBooklet
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Product promise

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