Human Integrin alpha 2 ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human Integrin alpha 2 in Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture supernatant samples.
Images
Key facts
- Detection method
- Colorimetric
- Sample types
- Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture supernatant
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 112.5 - 7200 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 34.3 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Integrin alpha-2/beta-1 is a receptor for laminin, collagen, collagen C-propeptides, fibronectin and E-cadherin. It recognizes the proline-hydroxylated sequence G-F-P-G-E-R in collagen. It is responsible for adhesion of platelets and other cells to collagens, modulation of collagen and collagenase gene expression, force generation and organization of newly synthesized extracellular matrix. (Microbial infection) Integrin ITGA2:ITGB1 acts as a receptor for Human rotavirus A. (Microbial infection) Integrin ITGA2:ITGB1 acts as a receptor for Human echoviruses 1 and 8.
Alternative names
CD49b, CD49B, ITGA2, Integrin alpha-2, CD49 antigen-like family member B, Collagen receptor, Platelet membrane glycoprotein Ia, VLA-2 subunit alpha, GPIa
What's included?
Human Integrin alpha 2 ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human Integrin alpha 2 in Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture supernatant samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture supernatant
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 112.5 - 7200 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 34.3 pg/mL
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Serum | n 8 | C.V. = 8.3 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Serum | n 3 | C.V. = 4.4 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 95 | Range 94 - 96 % |
Sample type Citrate plasma | Average % = 109 | Range 94 - 125 % |
Sample type EDTA Plasma | Average % = 110 | Range 103 - 115 % |
Sample type Heparin Plasma | Average % = 115 | Range 106 - 126 % |
Sample type Cell culture supernatant | Average % = 94 | Range 83 - 102 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human Integrin alpha 2 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Integrin alpha 2 protein in Serum, Cit Plasma, EDTA Plasma, Hep Plasma, Cell culture supernatant. Quantitate Human Integrin alpha 2 with 34.3 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibodyanalyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
Singlewash protocol reduces assay time to 90 minutes or less.
High sensitivity, specificity and reproducibility from superior antibodies.
Fully validated in biological samples.
96wells plate breakable into 12 x 8 wells strips, available in 14pack (10 x 96well plates).
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
Sandwich ELISA - Human Integrin alpha 2 ELISA Kit (ab323925)
Serum of ten individual healthy human female donors was measured in duplicate. Interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). The mean Integrin alpha 2 concentration was determined to be 75.12 ng/mL with a range of 34.54 – 103.82 ng/mL.
Sandwich ELISA - Human Integrin alpha 2 ELISA Kit (ab323925)
Interpolated concentration of native Integrin alpha 2 was measured in duplicate at different sample concentrations. Undiluted samples are as follows: serum 1:20, plasma (citrate) 1:25, plasma (EDTA) 1:67, and plasma (heparin) 1:33. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
Sandwich ELISA - Human Integrin alpha 2 ELISA Kit (ab323925)
The concentration of Integrin alpha 2 was measured in wild-type and knockout HeLa cell extracts based on a 100 µg/mL extract load in 1X Cell Extraction Buffer PTR (not provided with this kit, but available as Extraction Buffer 5X PTR ab193970). The mean concentration was determined to be 2,953.1 pg/mL in wild-type cell extract and undetectable in ITGA2 knockout cell extract. The wild-type and Integrin alpha 2 knockout cell extracts are available as Human ITGA2 (CD49b) knockout HeLa cell lysate ab257262.
Sandwich ELISA - Human Integrin alpha 2 ELISA Kit (ab323925)
Example of human Integrin alpha 2 standard curve. Background-subtracted data values (mean +/- SD) are graphed.
Downloads
Product protocols
- Visit the general protocols
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