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AB309447

Human Integrin beta 5 ELISA Kit

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Human Integrin beta 5 ELISA Kit is a Sandwich ELISA for the measurement of Human Integrin beta 5 in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

Integrin beta-5, ITGB5

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Sandwich ELISA - Human Integrin beta 5 ELISA Kit (AB309447)
  • sELISA

Supplier Data

Sandwich ELISA - Human Integrin beta 5 ELISA Kit (AB309447)

Example of standard curve using ab309447. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Serum, Plasma, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.07 ng/mL

Range

0.07 - 10 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Complementary Products

Biochemicals

AB142698

RGD (Arg-Gly-Asp), sequence involved in cell adhesion

Chemical Structure - RGD (Arg-Gly-Asp), sequence involved in cell adhesion (AB142698)

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Primary Antibodies

AB184312

Anti-Integrin beta 5 antibody [EPR19170]

RabMAb|Recombinant

Western blot - Anti-Integrin beta 5 antibody [EPR19170] (AB184312)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Integrin beta 5 ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Integrin beta 5 in serum, plasma, and cell culture supernatants. This assay employs an antibody specific for human Integrin beta 5 coated on a 96-well plate.
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Recovery

[ { "sample": "Serum", "range": "113 - 132 %", "average": "= 121.6" }, { "sample": "Plasma", "range": "89 - 104 %", "average": "= 98.14" }, { "sample": "Cell culture supernatant", "range": "74 - 87 %", "average": "= 82.12" }, { "sample": "Cell culture media", "range": "74 - 87 %", "average": "= 82.12" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab309447-1x96Tests", "assetComponentDetails": [ { "size":"2 x 1 Vial", "name":"Lyophilized Human Integrin beta 5 Protein Standard", "number":"AB309447-CMP03", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB309447-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human Integrin beta 5 Antibody-coated ELISA Plate", "number":"AB309447-CMP08", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent (Item E2)", "number":"AB309447-CMP04", "productcode":"" }, { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin", "number":"AB309447-CMP12", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human Integrin beta 5 Detection Antibody", "number":"AB309447-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB309447-CMP09", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB309447-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"ELISA Microplate Bag", "number":"AB309447-CMP07", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Integrin beta 5 also known as integrin ß5 is a protein that plays an important role in cell adhesion and signal transduction. It is a component of the integrin family known to mediate interactions between cells and the extracellular matrix (ECM). This protein with a molecular mass of around 90 kDa is expressed in various tissues predominantly in epithelial cells. Integrin beta 5 partners with the alpha V subunit to form the alpha V beta 5 integrin complex which facilitates specific ligand binding such as to vitronectin.
Biological function summary

Integrin beta 5 contributes to processes like cell migration proliferation and survival often occurring at cell surfaces. It is a part of the integrin complex which plays an important role in these cellular behaviors. Through its interaction with ECM proteins integrin beta 5 influences key biological activities and assists in maintaining tissue architecture. The activity of this integrin impacts how cells respond to their microenvironment influencing cellular fate decisions that are vital to growth and repair mechanisms.

Pathways

Several signaling pathways involve integrin beta 5. It actively participates in the MAPK/ERK and PI3K/AKT pathways which are essential for transmitting signals that promote cell proliferation and survival. Additionally this integrin works closely with other proteins such as focal adhesion kinase (FAK) and Src family kinases integrating signals that modulate cellular responses to mechanical and biochemical cues from the ECM.

Integrin beta 5 shows involvement in cancer pathogenesis and fibrotic diseases. In cancer its expression correlates with processes such as tumor cell invasion and metastasis impacting prognosis in various cancers including lung and breast cancer. The protein is related to other integrins and ECM components like integrin alpha V and fibronectin contributing to tumor progression. Additionally in fibrotic diseases the dysregulation of integrin beta 5 influences the remodeling of the ECM linking to proteins such as transforming growth factor-beta (TGF-beta) to facilitate pathological tissue changes.
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Product protocols

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Target data

Integrin alpha-V/beta-5 (ITGAV : ITGB5) is a receptor for fibronectin. It recognizes the sequence R-G-D in its ligand.. (Microbial infection) Integrin ITGAV : ITGB5 acts as a receptor for adenovirus type C.
See full target information Integrin beta-5
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websiteProtocolBooklet
en
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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