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AB326313

Human Interferon alpha 1 ELISA Kit, Chemiluminescent

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Human Interferon alpha 1 ELISA Kit, Chemiluminescent is a 55-min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Human Interferon alpha 1 with higher sensitivity and broader measuring range.

- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 0.27 - 600 pg/ml with a sensitivity of 0.15pg/mL.
- Design your own immunoassay: we also offer the conjugation-ready antibody pair.
-Patent pending

View Alternative Names

IFNA13, IFNA1, Interferon alpha-1/13, IFN-alpha-1/13, Interferon alpha-D, LeIF D

6 Images
Sandwich ELISA - Human Interferon alpha 1 ELISA Kit, Chemiluminescent (AB326313)
  • sELISA

Lab

Sandwich ELISA - Human Interferon alpha 1 ELISA Kit, Chemiluminescent (AB326313)

SimpleStep ELISA® Ignite technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 55 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

Sandwich ELISA - Human Interferon alpha 1 ELISA Kit, Chemiluminescent (AB326313)
  • sELISA

Lab

Sandwich ELISA - Human Interferon alpha 1 ELISA Kit, Chemiluminescent (AB326313)

Standard curve comparison between the Chemiluminescent Human Interferon alpha-1 SimpleStep ELISA and colorimetric Human Interferon alpha-1 SimpleStep ELISA (ab213479) in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Interferon alpha 1 ELISA Kit, Chemiluminescent (AB326313)
  • sELISA

Lab

Sandwich ELISA - Human Interferon alpha 1 ELISA Kit, Chemiluminescent (AB326313)

Dynamic range comparison between the Chemiluminescent Human Interferon alpha-1 SimpleStep ELISA, colorimetric Human Interferon alpha-1 SimpleStep ELISA (ab213479), and leading colorimetric competitor. The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and dynamic range.

Sandwich ELISA - Human Interferon alpha 1 ELISA Kit, Chemiluminescent (AB326313)
  • sELISA

Supplier Data

Sandwich ELISA - Human Interferon alpha 1 ELISA Kit, Chemiluminescent (AB326313)

Interpolated concentrations of Interferon alpha-1 in extract of HEK293T cells overexpressing human Interferon alpha-1 based on a 200 ng/mL extract load.

The concentrations of Interferon alpha-1 were measured in duplicate and interpolated from the Interferon alpha-1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD n=2). The mean Interferon alpha-1 concentration was determined to be 126 pg/mL in extract of HEK293T cells overexpressing Interferon alpha-1.

Sandwich ELISA - Human Interferon alpha 1 ELISA Kit, Chemiluminescent (AB326313)
  • sELISA

Supplier Data

Sandwich ELISA - Human Interferon alpha 1 ELISA Kit, Chemiluminescent (AB326313)

Interpolated concentrations of native Interferon alpha-1 in WHO International Standard INTERFERON ALPHA (Human leukocyte-derived) NIBSC 94/784 based on a 75 IU/mL load.

The concentrations of Interferon alpha-1 were measured in duplicate and interpolated from the Interferon alpha-1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD n=2). The mean Interferon alpha-1 concentration was determined to be 427.9 pg/mL in 75 IU/mL WHO International Standard INTERFERON ALPHA (Human leukocyte-derived) NIBSC 94/784.

Sandwich ELISA - Human Interferon alpha 1 ELISA Kit, Chemiluminescent (AB326313)
  • sELISA

Supplier Data

Sandwich ELISA - Human Interferon alpha 1 ELISA Kit, Chemiluminescent (AB326313)

Interpolated concentrations of Interferon alpha-1 in extracts of HEK293T cells overexpressing human Interferon alpha-1 and HEK293T cells transfected with the associated empty vector.

The concentrations of Interferon alpha-1 were measured in three different dilutions in duplicate and interpolated from the Interferon alpha-1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted in ng Interferon alpha-1 per mg of extract (mean +/- SD n=3). Interferon alpha-1 concentration was determined to be 647.6 ng/mg in extract of HEK293T cells overexpressing Interferon alpha-1. Values for the empty vector were below the minimum detectable dose.

Key facts

Detection method

Luminescent

Sample types

Serum, EDTA Plasma, Cell culture extracts, Heparin Plasma, Citrate plasma, Cell culture media

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.15 pg/mL

Range

0.27 - 600 pg/mL

Assay time

55m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

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Product details

Human Interferon alpha 1 ELISA Kit, Chemiluminescent (ab326313) is a rapid single-wash sandwich 55-min ELISA to measure Human Interferon alpha 1 in Serum, EDTA Plasma, Cell culture extracts, Heparin Plasma, Citrate plasma, Cell culture media with a sensitivity of 0.15 pg/mL.

How the assay works

Human Interferon alpha 1 ELISA Kit, Chemiluminescent (ab326313) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.

Assay Specificity

Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human Interferon alpha 1 (ab326313) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-IFN-alpha1 antibody [EPR18694-27] - BSA and Aside free (Capture) ab242952
- Anti-IFN-alpha1 antibody [EPR18694-3] - BSA and Aside free (Detector) ab242984
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Precision

[ { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 2" }, { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 3" } ]
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Recovery

[ { "sample": "Serum", "range": "87 - 92 %", "average": "= 89" }, { "sample": "EDTA Plasma", "range": "81 - 90 %", "average": "= 87" }, { "sample": "Cell culture extracts", "range": "102 - 108 %", "average": "= 105" }, { "sample": "Heparin Plasma", "range": "85 - 98 %", "average": "= 91" }, { "sample": "Citrate plasma", "range": "76 - 78 %", "average": "= 77" }, { "sample": "Cell culture media", "range": "92 - 94 %", "average": "= 93" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of two enzymes : a protein kinase and an oligoadenylate synthetase.
See full target information IFNA1
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websiteProtocolBooklet
en
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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