Human Interferon alpha 1 ELISA Kit, Fluorescent is a single-wash 90-min Simplestep used to quantify Human Interferon alpha 1 with a sensitivity of 1 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Fluorescent Sandwich ELISA - 530/570/590 nm readout : works on any standard plate reader
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Key facts
- Detection method
- Fluorescent
- Sample types
- Cell culture extracts, Tissue Extracts, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 1.8 - 1200 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 1 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase.
Alternative names
IFNA13, IFNA1, Interferon alpha-1/13, IFN-alpha-1/13, Interferon alpha-D, LeIF D
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Human Interferon alpha 1 ELISA Kit, Fluorescent is a single-wash 90-min Simplestep used to quantify Human Interferon alpha 1 with a sensitivity of 1 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Fluorescent Sandwich ELISA - 530/570/590 nm readout : works on any standard plate reader
Key facts
- Detection method
- Fluorescent
- Sample types
- Cell culture extracts, Tissue Extracts, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 1.8 - 1200 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 1 pg/mL
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample HEK-293T | n 3 | C.V. 2 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample HEK-293T | n 5 | C.V. 3 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 93 | Range 92 - 94 % |
Sample type Serum | Average % = 89 | Range 87 - 92 % |
Sample type EDTA Plasma | Average % = 87 | Range 81 - 90 % |
Sample type Cell culture extracts | Average % = 105 | Range 102 - 108 % |
Sample type Heparin Plasma | Average % = 91 | Range 85 - 98 % |
Sample type Citrate plasma | Average % = 77 | Range 76 - 78 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Interferon alpha-1 in vitro CatchPoint SimpleStep ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Interferon alpha-1 protein in human serum, plasma, cell culture supernatants, and cell and tissue extracts.
This CatchPoint SimpleStep ELISA kit has been optimized for Molecular Devices Microplate Readers. Click here for a list of recommended Microplate Readers.
If using a Molecular Devices' plate reader supported by SoftMax® Pro software, a preconfigured protocol for these CatchPoint SimpleStep ELISA Kits is available with all the protocol and analysis settings at www.softmaxpro.org.
The CatchPoint SimpleStep ELISA employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. CatchPoint HRP Development Solution containing the Stoplight Red Substrate is added. During incubation, the substrate is catalyzed by HRP generating a fluorescent product. Signal is generated proportionally to the amount of bound analyte and the intensity is measured in a fluorescence plater reader at 530/570/590 nm Excitation/Cutoff/Emission.
Interferon alpha-1 belongs to a group of functionally related but distinct proteins of interferon alpha family that share around 80% identity of their amino acid sequences. Members of the interferon-alpha family have both anti-viral and immunomodulatory activities on target cells.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Interferon alpha 1 (IFN-α1) is a type I interferon with significant antiviral and immunomodulatory activities. Alternate names include interferon alpha and IFNA1. This protein weighs approximately 19 kDa and expresses predominantly in leukocytes. It interacts with specific cell surface receptors to initiate various protective mechanisms in the cell. Researchers often study IFN-alpha-1 protein using techniques like interferon alpha ELISA and IFN-α ELISA for its effective quantification in experimental analyses.
- Biological function summary
Interferon alpha 1 functions as an important player in the immune response against viral infections. It belongs to a family of cytokines that signal through the JAK-STAT pathway to induce the expression of interferon-stimulated genes (ISGs). IFN-α1 operates independently and does not form large complexes with other proteins ensuring a focused response to pathogens. This protein's role is critical for the initiation of immune responses and limiting the spread of viruses within the host organism.
- Pathways
Interferon alpha 1 activates intracellular signaling cascades mainly the JAK-STAT pathway and NF-κB pathway. These pathways have important roles in cellular defense mechanisms. In the JAK-STAT pathway IFN-α1 interacts closely with other interferons and cytokines to enhance antiviral states. Through these pathways it shares functional relationships with proteins such as STAT1 and IRF7 which are critical for mounting effective antiviral defenses.
- Associated diseases and disorders
Interferon alpha 1 relates closely to conditions like hepatitis C and multiple sclerosis. It has therapeutic applications in these diseases due to its immunoregulatory effects. IFN-α1 interacts with various immune system components including major histocompatibility complex (MHC) proteins to modulate immune responses. Abnormalities in IFN-α1 signaling or expression can exacerbate inflammatory and autoimmune disorders linking it to both protective and pathological roles in human health.
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6 product images
Sandwich ELISA - Human Interferon alpha 1 ELISA Kit, Fluorescent (ab229432)
Example of human Interferon alpha-1 standard curve in Sample Diluent NS.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human Interferon alpha 1 ELISA Kit, Fluorescent (ab229432)
Interpolated concentrations of spike Interferon alpha-1 in human plasma samples.
The concentrations of Interferon alpha-1 were measured in duplicates, interpolated from the Interferon alpha-1 standard curves and corrected for sample dilution. Undiluted samples are as follows: plasma (EDTA) 100%, plasma (citrate) 100%, plasma (heparin) 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
Sandwich ELISA - Human Interferon alpha 1 ELISA Kit, Fluorescent (ab229432)
Interpolated concentrations of spike Interferon alpha-1 in human serum and cell culture media samples.
The concentrations of Interferon alpha-1 were measured in duplicates, interpolated from the Interferon alpha-1 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 100%, cell culture media (RPMI1640 + 10% FBS) 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
Sandwich ELISA - Human Interferon alpha 1 ELISA Kit, Fluorescent (ab229432)
Interpolated concentrations of Interferon alpha-1 in extract of HEK293T cells overexpressing human Interferon alpha-1 based on a 200 ng/mL extract load.
The concentrations of Interferon alpha-1 were measured in duplicate and interpolated from the Interferon alpha-1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Interferon alpha-1 concentration was determined to be 126 pg/mL in extract of HEK293T cells overexpressing Interferon alpha-1.
Sandwich ELISA - Human Interferon alpha 1 ELISA Kit, Fluorescent (ab229432)
Interpolated concentrations of native Interferon alpha-1 in WHO International Standard Interferon alpha (Human leukocyte-derived) NIBSC 94/784 based on a 75 IU/mL load.
The concentrations of Interferon alpha-1 were measured in duplicate and interpolated from the Interferon alpha-1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Interferon alpha-1 concentration was determined to be 427.9 pg/mL in 75 IU/mL WHO International Standard Interferon alpha (Human leukocyte-derived) NIBSC 94/784.
Sandwich ELISA - Human Interferon alpha 1 ELISA Kit, Fluorescent (ab229432)
Interpolated concentrations of Interferon alpha-1 in extracts of HEK293T cells overexpressing human Interferon alpha-1 and HEK293T cells transfected with the associated empty vector.
The concentrations of Interferon alpha-1 were measured in three different dilutions in duplicate and interpolated from the Interferon alpha-1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted in ng Interferon alpha-1 per mg of extract (mean +/- SD, n=3). Interferon alpha-1 concentration was determined to be 647.6 ng/mg in extract of HEK293T cells overexpressing Interferon alpha-1. Values for the empty vector were below the minimum detectable dose.
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