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AB229432

Human Interferon alpha 1 ELISA Kit, Fluorescent

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Human Interferon alpha 1 ELISA Kit, Fluorescent is a single-wash 90-min Simplestep used to quantify Human Interferon alpha 1 with a sensitivity of 1 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Fluorescent Sandwich ELISA - 530/570/590 nm readout : works on any standard plate reader

View Alternative Names

IFNA13, IFNA1, Interferon alpha-1/13, IFN-alpha-1/13, Interferon alpha-D, LeIF D

6 Images
Sandwich ELISA - Human Interferon alpha 1 ELISA Kit, Fluorescent (AB229432)
  • sELISA

Supplier Data

Sandwich ELISA - Human Interferon alpha 1 ELISA Kit, Fluorescent (AB229432)

Interpolated concentrations of Interferon alpha-1 in extracts of HEK293T cells overexpressing human Interferon alpha-1 and HEK293T cells transfected with the associated empty vector.

The concentrations of Interferon alpha-1 were measured in three different dilutions in duplicate and interpolated from the Interferon alpha-1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted in ng Interferon alpha-1 per mg of extract (mean +/- SD, n=3). Interferon alpha-1 concentration was determined to be 647.6 ng/mg in extract of HEK293T cells overexpressing Interferon alpha-1. Values for the empty vector were below the minimum detectable dose.

Sandwich ELISA - Human Interferon alpha 1 ELISA Kit, Fluorescent (AB229432)
  • sELISA

Supplier Data

Sandwich ELISA - Human Interferon alpha 1 ELISA Kit, Fluorescent (AB229432)

Interpolated concentrations of spike Interferon alpha-1 in human serum and cell culture media samples.

The concentrations of Interferon alpha-1 were measured in duplicates, interpolated from the Interferon alpha-1 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 100%, cell culture media (RPMI1640 + 10% FBS) 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Sandwich ELISA - Human Interferon alpha 1 ELISA Kit, Fluorescent (AB229432)
  • sELISA

Supplier Data

Sandwich ELISA - Human Interferon alpha 1 ELISA Kit, Fluorescent (AB229432)

Interpolated concentrations of spike Interferon alpha-1 in human plasma samples.

The concentrations of Interferon alpha-1 were measured in duplicates, interpolated from the Interferon alpha-1 standard curves and corrected for sample dilution. Undiluted samples are as follows : plasma (EDTA) 100%, plasma (citrate) 100%, plasma (heparin) 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Sandwich ELISA - Human Interferon alpha 1 ELISA Kit, Fluorescent (AB229432)
  • sELISA

Supplier Data

Sandwich ELISA - Human Interferon alpha 1 ELISA Kit, Fluorescent (AB229432)

Example of human Interferon alpha-1 standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Interferon alpha 1 ELISA Kit, Fluorescent (AB229432)
  • sELISA

Supplier Data

Sandwich ELISA - Human Interferon alpha 1 ELISA Kit, Fluorescent (AB229432)

Interpolated concentrations of native Interferon alpha-1 in WHO International Standard Interferon alpha (Human leukocyte-derived) NIBSC 94/784 based on a 75 IU/mL load.

The concentrations of Interferon alpha-1 were measured in duplicate and interpolated from the Interferon alpha-1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Interferon alpha-1 concentration was determined to be 427.9 pg/mL in 75 IU/mL WHO International Standard Interferon alpha (Human leukocyte-derived) NIBSC 94/784.

Sandwich ELISA - Human Interferon alpha 1 ELISA Kit, Fluorescent (AB229432)
  • sELISA

Supplier Data

Sandwich ELISA - Human Interferon alpha 1 ELISA Kit, Fluorescent (AB229432)

Interpolated concentrations of Interferon alpha-1 in extract of HEK293T cells overexpressing human Interferon alpha-1 based on a 200 ng/mL extract load.

The concentrations of Interferon alpha-1 were measured in duplicate and interpolated from the Interferon alpha-1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Interferon alpha-1 concentration was determined to be 126 pg/mL in extract of HEK293T cells overexpressing Interferon alpha-1.

Key facts

Detection method

Fluorescent

Sample types

Cell culture extracts, Tissue Extracts, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 1 pg/mL

Range

1.8 - 1200 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Interferon alpha-1 in vitro CatchPoint SimpleStep ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Interferon alpha-1 protein in human serum, plasma, cell culture supernatants, and cell and tissue extracts.

This CatchPoint SimpleStep ELISA kit has been optimized for Molecular Devices Microplate Readers. Click here for a list of recommended Microplate Readers.
If using a Molecular Devices' plate reader supported by SoftMax® Pro software, a preconfigured protocol for these CatchPoint SimpleStep ELISA Kits is available with all the protocol and analysis settings at www.softmaxpro.org.

The CatchPoint SimpleStep ELISA employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. CatchPoint HRP Development Solution containing the Stoplight Red Substrate is added. During incubation, the substrate is catalyzed by HRP generating a fluorescent product. Signal is generated proportionally to the amount of bound analyte and the intensity is measured in a fluorescence plater reader at 530/570/590 nm Excitation/Cutoff/Emission.

Interferon alpha-1 belongs to a group of functionally related but distinct proteins of interferon alpha family that share around 80% identity of their amino acid sequences. Members of the interferon-alpha family have both anti-viral and immunomodulatory activities on target cells.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "HEK-293T", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3" }, { "reproducibilityType": "Intra", "sample": "HEK-293T", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "92 - 94 %", "average": "= 93" }, { "sample": "Serum", "range": "87 - 92 %", "average": "= 89" }, { "sample": "EDTA Plasma", "range": "81 - 90 %", "average": "= 87" }, { "sample": "Cell culture extracts", "range": "102 - 108 %", "average": "= 105" }, { "sample": "Heparin Plasma", "range": "85 - 98 %", "average": "= 91" }, { "sample": "Citrate plasma", "range": "76 - 78 %", "average": "= 77" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Interferon alpha 1 (IFN-α1) is a type I interferon with significant antiviral and immunomodulatory activities. Alternate names include interferon alpha and IFNA1. This protein weighs approximately 19 kDa and expresses predominantly in leukocytes. It interacts with specific cell surface receptors to initiate various protective mechanisms in the cell. Researchers often study IFN-alpha-1 protein using techniques like interferon alpha ELISA and IFN-α ELISA for its effective quantification in experimental analyses.
Biological function summary

Interferon alpha 1 functions as an important player in the immune response against viral infections. It belongs to a family of cytokines that signal through the JAK-STAT pathway to induce the expression of interferon-stimulated genes (ISGs). IFN-α1 operates independently and does not form large complexes with other proteins ensuring a focused response to pathogens. This protein's role is critical for the initiation of immune responses and limiting the spread of viruses within the host organism.

Pathways

Interferon alpha 1 activates intracellular signaling cascades mainly the JAK-STAT pathway and NF-κB pathway. These pathways have important roles in cellular defense mechanisms. In the JAK-STAT pathway IFN-α1 interacts closely with other interferons and cytokines to enhance antiviral states. Through these pathways it shares functional relationships with proteins such as STAT1 and IRF7 which are critical for mounting effective antiviral defenses.

Interferon alpha 1 relates closely to conditions like hepatitis C and multiple sclerosis. It has therapeutic applications in these diseases due to its immunoregulatory effects. IFN-α1 interacts with various immune system components including major histocompatibility complex (MHC) proteins to modulate immune responses. Abnormalities in IFN-α1 signaling or expression can exacerbate inflammatory and autoimmune disorders linking it to both protective and pathological roles in human health.
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Product protocols

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Target data

Produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of two enzymes : a protein kinase and an oligoadenylate synthetase.
See full target information IFNA1
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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