AB233622

Human Interferon Alpha 2 ELISA Kit

Recombinant
SimpleStep
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(1 Publication)

Human Interferon Alpha 2 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Interferon Alpha 2 with a sensitivity of 56.04 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

IFNA2A, IFNA2B, IFNA2C, IFNA2, Interferon alpha-2, IFN-alpha-2, Interferon alpha-A, LeIF A

2 Images

Sandwich ELISA - Human Interferon Alpha 2 ELISA Kit (AB233622)

sELISA

Supplier Data

Sandwich ELISA - Human Interferon Alpha 2 ELISA Kit (AB233622)

Interpolated concentrations of spiked Interferon Alpha 2 in human serum, plasma and culture media samples.

The concentrations of Interferon Alpha 2 were measured in duplicates, interpolated from the Interferon Alpha 2 standard curves.

sELISA

Supplier Data

Sandwich ELISA - Human Interferon Alpha 2 ELISA Kit (AB233622)

Example of human Interferon Alpha 2 standard curve in Sample Diluent NS.

Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Citrate plasma, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 56.04 pg/mL

Range

187.5 - 12000 pg/mL

Assay time

1h 30m

Assay Platform

Microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human Interferon Alpha 2 ELISA Kit (ab233622) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Interferon Alpha 2 protein in cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Interferon Alpha 2 with 56.04 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "9.3" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.7" } ]

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Recovery

[ { "sample": "Serum", "range": "99 - 109 %", "average": "= 103" }, { "sample": "EDTA Plasma", "range": "80 - 93 %", "average": "= 87" }, { "sample": "Heparin Plasma", "range": "94 - 108 %", "average": "= 104" }, { "sample": "Citrate plasma", "range": "102 - 103 %", "average": "= 103" }, { "sample": "Cell culture media", "range": "100 - 116 %", "average": "= 106" } ]

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What's included?

{ "values": { "10x96Tests": { "sellingSize": "10 x 96 Tests", "publicAssetCode":"ab233622-10x96Tests", "assetComponentDetails": [ { "size":"1 x 120 mL", "name":"Stop Solution", "number":"AB233622-CMP06" }, { "size":"1 x 200 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB233622-CMP10" }, { "size":"1 x 120 mL", "name":"TMB Development Solution", "number":"AB233622-CMP07" }, { "size":"2 x 250 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB233622-CMP04" }, { "size":"2 x 10 Vial", "name":"Human Interferon Alpha 2 Lyophilized Purified Protein", "number":"AB233622-CMP08" }, { "size":"1 x 6000 µL", "name":"10X Human Interferon Alpha 2 Capture Antibody", "number":"AB233622-CMP02" }, { "size":"10 x 6 mL", "name":"Antibody Diluent 5BI", "number":"AB233622-CMP01" }, { "size":"10 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB233622-CMP05" }, { "size":"1 x 6000 µL", "name":"10X Human Interferon Alpha 2 Detector Antibody", "number":"AB233622-CMP03" }, { "size":"10 x 1 Unit", "name":"Plate Seals", "number":"AB233622-CMP09" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab233622-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB233622-CMP06", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB233622-CMP10", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB233622-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB233622-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB233622-CMP09", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BI", "number":"AB233622-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Interferon Alpha 2 Lyophilized Purified Protein", "number":"AB233622-CMP08", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Interferon Alpha 2 Capture Antibody", "number":"AB233622-CMP02", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Interferon Alpha 2 Detector Antibody", "number":"AB233622-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB233622-CMP05", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Interferon alpha 2 also known as IFN alpha 2 or IFN alpha 2a/b is a type of cytokine that plays a critical role in the immune response. It is a smaller protein weighing approximately 19.5 kDa mainly expressed by leukocytes such as macrophages and dendritic cells. This protein exhibits antiviral antiproliferative and immunomodulatory activities by binding to specific receptors on target cells. Researchers often study interferon alpha through recombinant protein studies which can be simulated by companies like PeproTech. Additionally ELISA assays facilitate the quantitative measurement of interferon alpha levels in various biological samples.

Biological function summary

Interferon alpha 2 engages in complex signaling with other immune factors to establish an antiviral state in cells. This protein binds to the IFNAR1 and IFNAR2 receptor complex initiating a cascade that activates genes responsible for immune defense. By doing so it triggers the transcription of various antiviral proteins and enhances the activity of immune cells including natural killer cells and macrophages. Its function is also evaluated in reconstitution studies such as PeproTech's IL-2 reconstitution to investigate broader immune functions.

Pathways

Researchers observe interferon alpha 2 predominantly within the JAK-STAT signaling pathway. This pathway is vital in transmitting the extracellular signal through to the gene transcription process leading to an appropriate immune response. STAT proteins such as STAT1 and STAT2 form a transcription complex following activation by JAK kinases. Interferon alpha 2's interactions in the pathway exemplify how this cytokine links with other components for immune regulation coordinating with other proteins like ISGF3 to amplify antiviral responses.

Scientists associate interferon alpha 2 with chronic viral infections and certain cancers. For example its therapeutic application has been prominent in treating hepatitis C and some hematological malignancies. In viral infections interferon alpha inhibits viral replication and enhances antiviral immunity. In cancer it suppresses cell proliferation and regulates immune surveillance. The protein's levels and activity can correlate with pathological states influenced by other proteins such as IFN-lambda and TNF-alpha highlighting its role in disease mechanisms and therapeutic interventions.

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Product protocols

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Target data

Produced by macrophages, IFN-alpha have antiviral activities.

See full target information IFNA2

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell reports. Medicine 4:100894 PubMed36652906

2023

Inactive disease in patients with lupus is linked to autoantibodies to type I interferons that normalize blood IFNα and B cell subsets.

Applications

Unspecified application

Species

Unspecified reactive species

Hannah F Bradford,Liis Haljasmägi,Madhvi Menon,Thomas C R McDonnell,Karita Särekannu,Martti Vanker,Pärt Peterson,Chris Wincup,Rym Abida,Raquel Fernandez Gonzalez,Vincent Bondet,Darragh Duffy,David A Isenberg,Kai Kisand,Claudia Mauri

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Human Interferon Alpha 2 ELISA Kit

Sandwich ELISA - Human Interferon Alpha 2 ELISA Kit (AB233622)

Sandwich ELISA - Human Interferon Alpha 2 ELISA Kit (AB233622)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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Secondary Antibodies

Primary Antibodies

Product promise