Human Interferon Alpha 2 ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human Interferon Alpha 2 in Heparin Plasma, Citrate plasma, Serum, EDTA Plasma samples.
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- Cell reports. Medicine 4:1008942023Inactive disease in patients with lupus is linked to autoantibodies to type I interferons that normalize blood IFNα and B cell subsets.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHannah F Bradford,Liis Haljasmägi,Madhvi Menon,Thomas C R McDonnell,Karita Särekannu,Martti Vanker,Pärt Peterson,Chris Wincup,Rym Abida,Raquel Fernandez Gonzalez,Vincent Bondet,Darragh Duffy,David A Isenberg,Kai Kisand,Claudia MauriPubMed 36652906
Key facts
- Detection method
Colorimetric
- Sample types
Heparin Plasma, Citrate plasma, Serum, EDTA Plasma
- Assay type
Sandwich (quantitative)
- Reactive species
Human
- Range
187.5 - 12000 pg/mL
- Assay time
1h 30m
- Sensitivity
= 56.04 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
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Target data
Function
Produced by macrophages, IFN-alpha have antiviral activities.
Alternative names
Interferon alpha-2, IFN-alpha-2, Interferon alpha-A, LeIF A, IFNA2C, IFNA2B, IFNA2A, IFNA2
What's included?
Recommended products
Human Interferon Alpha 2 ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human Interferon Alpha 2 in Heparin Plasma, Citrate plasma, Serum, EDTA Plasma samples.
Key facts
- Detection method
Colorimetric
- Sample types
Heparin Plasma, Citrate plasma, Serum, EDTA Plasma
- Assay type
Sandwich (quantitative)
- Reactive species
Human
- Range
187.5 - 12000 pg/mL
- Assay time
1h 30m
- Assay Platform
Microplate (12 x 8 well strips)
- Sensitivity
= 56.04 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 3 | mean - | SD - | C.V. 2.7 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 8 | mean - | SD - | C.V. 9.3 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 103 | Range 99 - 109 % |
Sample type EDTA Plasma | Average % = 87 | Range 80 - 93 % |
Sample type Heparin Plasma | Average % = 104 | Range 94 - 108 % |
Sample type Citrate plasma | Average % = 103 | Range 102 - 103 % |
Sample type Cell culture media | Average % = 106 | Range 100 - 116 % |
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
+4°C
- Storage information
+4°C
Notes
Human Interferon Alpha 2 ELISA Kit (ab233622) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Interferon Alpha 2 protein in cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Interferon Alpha 2 with 56.04 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Interferon alpha 2 also known as IFN alpha 2 or IFN alpha 2a/b is a type of cytokine that plays a critical role in the immune response. It is a smaller protein weighing approximately 19.5 kDa mainly expressed by leukocytes such as macrophages and dendritic cells. This protein exhibits antiviral antiproliferative and immunomodulatory activities by binding to specific receptors on target cells. Researchers often study interferon alpha through recombinant protein studies which can be simulated by companies like PeproTech. Additionally ELISA assays facilitate the quantitative measurement of interferon alpha levels in various biological samples.
- Biological function summary
Interferon alpha 2 engages in complex signaling with other immune factors to establish an antiviral state in cells. This protein binds to the IFNAR1 and IFNAR2 receptor complex initiating a cascade that activates genes responsible for immune defense. By doing so it triggers the transcription of various antiviral proteins and enhances the activity of immune cells including natural killer cells and macrophages. Its function is also evaluated in reconstitution studies such as PeproTech's IL-2 reconstitution to investigate broader immune functions.
- Pathways
Researchers observe interferon alpha 2 predominantly within the JAK-STAT signaling pathway. This pathway is vital in transmitting the extracellular signal through to the gene transcription process leading to an appropriate immune response. STAT proteins such as STAT1 and STAT2 form a transcription complex following activation by JAK kinases. Interferon alpha 2's interactions in the pathway exemplify how this cytokine links with other components for immune regulation coordinating with other proteins like ISGF3 to amplify antiviral responses.
- Associated diseases and disorders
Scientists associate interferon alpha 2 with chronic viral infections and certain cancers. For example its therapeutic application has been prominent in treating hepatitis C and some hematological malignancies. In viral infections interferon alpha inhibits viral replication and enhances antiviral immunity. In cancer it suppresses cell proliferation and regulates immune surveillance. The protein's levels and activity can correlate with pathological states influenced by other proteins such as IFN-lambda and TNF-alpha highlighting its role in disease mechanisms and therapeutic interventions.
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2 product images
Sandwich ELISA - Human Interferon Alpha 2 ELISA Kit (ab233622)
Example of human Interferon Alpha 2 standard curve in Sample Diluent NS.
Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human Interferon Alpha 2 ELISA Kit (ab233622)
Interpolated concentrations of spiked Interferon Alpha 2 in human serum, plasma and culture media samples.
The concentrations of Interferon Alpha 2 were measured in duplicates, interpolated from the Interferon Alpha 2 standard curves.
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