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AB278127

Human Interferon beta ELISA Kit

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(3 Publications)

Human Interferon beta ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Interferon beta with a sensitivity of 2 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

IFB, IFNB, IFNB1, Interferon beta, IFN-beta, Fibroblast interferon

3 Images
ELISA - Human Interferon beta ELISA Kit (AB278127)
  • ELISA

Supplier Data

ELISA - Human Interferon beta ELISA Kit (AB278127)
Sandwich ELISA - Human Interferon beta ELISA Kit (AB278127)
  • sELISA

Supplier Data

Sandwich ELISA - Human Interferon beta ELISA Kit (AB278127)

Interpolated concentrations of native Interferon beta in human A549 cell culture supernatant samples transfected with 400 ng/mL or 200 ng/mL Poly I : C for 24 hours.

The concentrations of Interferon beta were measured in duplicates, interpolated from the Interferon beta standard curves and corrected for sample dilution. Undiluted samples are as follows : A549 cell culture supernatant transfected with 400 ng/mL Poly I : C 100%, A549 cell culture supernatant transfected with 200 ng/mL Poly I : C 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Interferon beta concentration was determined to be 111.4 pg/mL in neat A549 supernatant transfected with 400 ng/mL Poly I : C and 33.7 pg/mL in neat A549 supernatant transfected with 200 ng/mL Poly I : C. There was no detectable signal in mock treated A549 supernatant samples.

Sandwich ELISA - Human Interferon beta ELISA Kit (AB278127)
  • sELISA

Supplier Data

Sandwich ELISA - Human Interferon beta ELISA Kit (AB278127)

Example of human Interferon beta standard curve in Sample Diluent NS.

The Interferon beta standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 2 pg/mL

Range

9.38 - 600 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Interferon beta ELISA Kit ab278127 is a rapid single-wash 90-min Sandwich ELISA to measure Human Interferon beta in cell culture supernatant, citrate plasma, EDTA plasma, heparin plasma, serum. This SimpleStep sensitivity is 2 pg/mL.

How the assay works

Human Interferon beta SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human Interferon beta ELISA Kit ab278127 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-Interferon beta antibody [EPR23341-76] - BSA and Azide free (Capture) ab274352
- Anti-Interferon beta antibody [EPR23341-36] - BSA and Azide free (Detector) ab274352

Human Interferon beta is a 166 amino acids long secreted cytokine that is produced by fibroblasts, dendritic cells, macrophages, and endothelial cells upon pathogen exposure. Interferon beta is type I interferon which mainly helps to stimulate anti-viral response. Interferon beta signals through the heterodimeric IFN-alpha/beta Receptor and activates the JAK/STAT signaling pathway.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "83 - 106 %", "average": "= 96" }, { "sample": "Serum", "range": "89 - 97 %", "average": "= 92" }, { "sample": "EDTA Plasma", "range": "97 - 106 %", "average": "= 101" }, { "sample": "Heparin Plasma", "range": "112 - 119 %", "average": "= 116" }, { "sample": "Citrate plasma", "range": "114 - 120 %", "average": "= 117" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab278127-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB278127-CMP06", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB278127-CMP11", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB278127-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB278127-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB278127-CMP10", "productcode":"" }, { "size":"1 x 20 mL", "name":"Sample Diluent 50BS", "number":"AB278127-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Interferon beta Capture Antibody", "number":"AB278127-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Interferon beta Lyophilized Recombinant Protein", "number":"AB278127-CMP09", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB278127-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB278127-CMP05", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Interferon beta Detector Antibody", "number":"AB278127-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Interferon beta also known as IFN-beta or beta interferon is a type I interferon with a mass of approximately 20 kDa. It originates primarily from fibroblasts and plays an essential role in the immune response. This protein targets and binds to specific cell surface receptors initiating various antiviral states within cells. This action assists in slowing down viral replication and spread. IFN-beta's expression increases in response to viral infections making it an important component in the body's first line of defense against pathogens.
Biological function summary

Interferon beta functions as an important mediator of immune functions regulating the activity of natural killer cells and macrophages. It also boosts antigen presentation to T cells. Interferon beta belongs to the larger family of interferons which includes IFN-alpha and IFN-gamma each having distinct effects but working together to mount an effective immune response. Though it does not form part of a complex it plays a critical standalone role in immune signaling pathways.

Pathways

Interferon beta associates strongly with the JAK-STAT signaling pathway. Upon activation it interacts with receptors to phosphorylate STAT proteins mainly STAT1 and STAT2 which then dimerize and translocate to the nucleus to trigger gene expression. Another important pathway is the antiviral response where interferon beta modulates the expression of hundreds of interferon-stimulated genes. These pathways intricately involve other proteins like IFN-alpha and IFN-gamma which work synergistically to establish an effective antiviral environment.

Interferon beta shows significant implications in multiple sclerosis and hepatitis C infection. It is widely used as a therapeutic agent in managing multiple sclerosis by modulating the immune response to reduce inflammation and slow disease progression. Similarly its role in antiviral defense makes it relevant to hepatitis C infection management. In these contexts IFN-alpha and IFN-gamma are also important as they share similar antiviral and immunomodulatory functions that can complement IFN-beta's actions enhancing the treatment's efficacy.
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Product protocols

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Target data

Type I interferon cytokine that plays a key role in the innate immune response to infection, developing tumors and other inflammatory stimuli (PubMed : 10049744, PubMed : 10556041, PubMed : 6157094, PubMed : 6171735, PubMed : 7665574, PubMed : 8027027, PubMed : 8969169). Signals via binding to high-affinity (IFNAR2) and low-affinity (IFNAR1) heterodimeric receptor, activating the canonical Jak-STAT signaling pathway resulting in transcriptional activation or repression of interferon-regulated genes that encode the effectors of the interferon response, such as antiviral proteins, regulators of cell proliferation and differentiation, and immunoregulatory proteins (PubMed : 10049744, PubMed : 10556041, PubMed : 7665574, PubMed : 8027027, PubMed : 8969169). Signals mostly via binding to a IFNAR1-IFNAR2 heterodimeric receptor, but can also function with IFNAR1 alone and independently of Jak-STAT pathways (By similarity). Elicits a wide variety of responses, including antiviral and antibacterial activities, and can regulate the development of B-cells, myelopoiesis and lipopolysaccharide (LPS)-inducible production of tumor necrosis factor (By similarity). Plays a role in neuronal homeostasis by regulating dopamine turnover and protecting dopaminergic neurons : acts by promoting neuronal autophagy and alpha-synuclein clearance, thereby preventing dopaminergic neuron loss (By similarity). IFNB1 is more potent than interferon-alpha (IFN-alpha) in inducing the apoptotic and antiproliferative pathways required for control of tumor cell growth (By similarity).
See full target information IFNB1
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Heliyon 10:e29338 PubMed38665565

2024

Multiple inborn errors of type I IFN immunity in a 33-year-old male with a fatal case of COVID-19.

Applications

Unspecified application

Species

Unspecified reactive species

Narjes Saheb Sharif-Askari,Shirin Hafezi,Fatemeh Saheb Sharif-Askari,Hawra Ali Hussain Alsayed,Samrein B M Ahmed,Habiba S Alsafar,Rabih Halwani

iScience 27:109213 PubMed38439953

2024

CD169 classical monocyte as an important participant in Graves' ophthalmopathy through CXCL12-CXCR4 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Dongliang Wang,Jie Ling,RongQiang Tan,Huishi Wang,Yixin Qu,Xingyi Li,Jinshan Lin,Qikai Zhang,Qiuling Hu,Zhong Liu,Zhaojing Lu,Yuheng Lin,Li Sun,Dingqiao Wang,Ming Zhou,Zhuoxing Shi,Wuyou Gao,Huijing Ye,Xianchai Lin

Cell metabolism 35:1782-1798.e8 PubMed37586363

2023

NSUN2 is a glucose sensor suppressing cGAS/STING to maintain tumorigenesis and immunotherapy resistance.

Applications

Unspecified application

Species

Unspecified reactive species

Tingjin Chen,Zhi-Gang Xu,Jie Luo,Rajesh Kumar Manne,Zhengyu Wang,Che-Chia Hsu,Bo-Syong Pan,Zhen Cai,Pei-Jane Tsai,Yau-Sheng Tsai,Zhong-Zhu Chen,Hong-Yu Li,Hui-Kuan Lin
View all publications
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