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AB325737

Human Interferon beta ELISA Kit, Chemiluminescent

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Human Interferon beta ELISA Kit, Chemiluminescent is a 55-min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Human Interferon beta with higher sensitivity and broader measuring range.

- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 0.73 - 1600 pg/ml with a sensitivity of 0.34pg/mL.
- Design your own immunoassay: we also offer the conjugation-ready antibody pair.
-Patent pending

View Alternative Names

IFB, IFNB, IFNB1, Interferon beta, IFN-beta, Fibroblast interferon

4 Images
Sandwich ELISA - Human Interferon beta ELISA Kit, Chemiluminescent (AB325737)
  • sELISA

Lab

Sandwich ELISA - Human Interferon beta ELISA Kit, Chemiluminescent (AB325737)

SimpleStep ELISA® Ignite technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 55 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

Sandwich ELISA - Human Interferon beta ELISA Kit, Chemiluminescent (AB325737)
  • sELISA

Lab

Sandwich ELISA - Human Interferon beta ELISA Kit, Chemiluminescent (AB325737)

Standard curve comparison between the Chemiluminescent Human Interferon beta SimpleStep ELISA and colorimetric Human Interferon beta SimpleStep ELISA (ab278127) in Sample Diluent 50BS. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Interferon beta ELISA Kit, Chemiluminescent (AB325737)
  • sELISA

Lab

Sandwich ELISA - Human Interferon beta ELISA Kit, Chemiluminescent (AB325737)

Dynamic range comparison between the Chemiluminescent Human Interferon beta SimpleStep ELISA, colorimetric Human Interferon beta SimpleStep ELISA (ab278127), and leading colorimetric competitor. The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and increased dynamic range.

Sandwich ELISA - Human Interferon beta ELISA Kit, Chemiluminescent (AB325737)
  • sELISA

Supplier Data

Sandwich ELISA - Human Interferon beta ELISA Kit, Chemiluminescent (AB325737)

Interpolated concentrations of native Interferon beta in human A549 cell culture supernatant samples transfected with 400 ng/mL or 200 ng/mL Poly I : C for 24 hours.

The concentrations of Interferon beta were measured in duplicates interpolated from the Interferon beta standard curves and corrected for sample dilution. Undiluted samples are as follows : A549 cell culture supernatant transfected with 400 ng/mL Poly I : C 100% A549 cell culture supernatant transfected with 200 ng/mL Poly I : C 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD n=2). The mean Interferon beta concentration was determined to be 111.4 pg/mL in neat A549 supernatant transfected with 400 ng/mL Poly I : C and 33.7 pg/mL in neat A549 supernatant transfected with 200 ng/mL Poly I : C. There was no detectable signal in mock treated A549 supernatant samples.

Key facts

Detection method

Luminescent

Sample types

Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.34 pg/mL

Range

0.73 - 1600 pg/mL

Assay time

55m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

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Product details

Human Interferon beta ELISA Kit, Chemiluminescent (ab325737) is a rapid single-wash sandwich 55-min ELISA to measure Human Interferon beta in Serum, Plasma – Citrate, Plasma – EDTA, Plasma – Heparin, Cell Culture Supernatant with a sensitivity of 0.34 pg/mL.

How the assay works

Human Interferon beta ELISA Kit, Chemiluminescent (ab325737) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.

Assay Specificity

Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human Interferon beta (ab325737) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-IFNB1 antibody [EPR23341-76] - BSA and Aside free (Capture) ab274353
- Anti-IFNB1 antibody [EPR23341-36] - BSA and Aside free (Detector) ab274352
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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 7" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 2" } ]
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Recovery

[ { "sample": "Serum", "range": "89 - 97 %", "average": "= 92" }, { "sample": "Citrate plasma", "range": "114 - 120 %", "average": "= 117" }, { "sample": "EDTA Plasma", "range": "97 - 106 %", "average": "= 101" }, { "sample": "Heparin Plasma", "range": "112 - 119 %", "average": "= 116" }, { "sample": "Cell culture supernatant", "range": "83 - 106 %", "average": "= 96" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Type I interferon cytokine that plays a key role in the innate immune response to infection, developing tumors and other inflammatory stimuli (PubMed : 10049744, PubMed : 10556041, PubMed : 6157094, PubMed : 6171735, PubMed : 7665574, PubMed : 8027027, PubMed : 8969169). Signals via binding to high-affinity (IFNAR2) and low-affinity (IFNAR1) heterodimeric receptor, activating the canonical Jak-STAT signaling pathway resulting in transcriptional activation or repression of interferon-regulated genes that encode the effectors of the interferon response, such as antiviral proteins, regulators of cell proliferation and differentiation, and immunoregulatory proteins (PubMed : 10049744, PubMed : 10556041, PubMed : 7665574, PubMed : 8027027, PubMed : 8969169). Signals mostly via binding to a IFNAR1-IFNAR2 heterodimeric receptor, but can also function with IFNAR1 alone and independently of Jak-STAT pathways (By similarity). Elicits a wide variety of responses, including antiviral and antibacterial activities, and can regulate the development of B-cells, myelopoiesis and lipopolysaccharide (LPS)-inducible production of tumor necrosis factor (By similarity). Plays a role in neuronal homeostasis by regulating dopamine turnover and protecting dopaminergic neurons : acts by promoting neuronal autophagy and alpha-synuclein clearance, thereby preventing dopaminergic neuron loss (By similarity). IFNB1 is more potent than interferon-alpha (IFN-alpha) in inducing the apoptotic and antiproliferative pathways required for control of tumor cell growth (By similarity).
See full target information IFNB1
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websiteProtocolBooklet
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Product promise

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For full details, please see our Terms & Conditions

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