Human IP-10 ELISA Kit ab173194 is a single-wash 90-min SimpleStep ELISA used to quantify Human IP-10 with a sensitivity of 2.6 pg/ml. The assay uses a simple Mix-Wash-Read protocol with just one incubation and wash step.
Easy-to-use, rapid, sensitive Human IP-10 sandwich ELISA Kit:
- Mix, Wash, Read: get results in 90 minutes with SimpleStep ELISA® format
- Colorimetric assay - 450nm readout; works on any plate reader
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- EMBO reports 23:e558712022Endothelial-immune crosstalk contributes to vasculopathy in nonalcoholic fatty liver disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChun-Yi Ng,Khang Leng Lee,Mark Dhinesh Muthiah,Kan Xing Wu,Florence Wen Jing Chioh,Konstanze Tan,Gwyneth Shook Ting Soon,Asim Shabbir,Wai Mun Loo,Zun Siong Low,Qingfeng Chen,Walter Wahli,Nguan Soon Tan,Huck Hui Ng,Yock Young Dan,Christine CheungPubMed 36200589
- Frontiers in medicine 9:8964092022Alterations in bile acids as metabolic signatures in the patients with human adenovirus type 7 infection.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWen Xu,Juan Du,Ting-Ting Wei,Lin-Yi Chen,Xin-Xin Yang,Tu Bo,Han-Yu Liu,Ming-Zhu Xie,Tian-Shuo Zhao,Jun-Lian Yang,Fuqiang Cui,Wei-Wei Chen,Qing-Bin LuPubMed 36160124
- Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 37:1843-18492022CXCL9 Predicts the Risk of Osteoporotic Hip Fracture in a Prospective Cohort of Chinese Men-A Matched Case-Control Study.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQuang Tien Phan,Kevin Yiqiang Chua,Aizhen Jin,Christoph Winkler,Woon-Puay KohPubMed 35810382
- Clinical and translational medicine 12:e8022022Deciphering the quality of SARS-CoV-2 specific T-cell response associated with disease severity, immune memory and heterologous response.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlberto Pérez-Gómez,Carmen Gasca-Capote,Joana Vitallé,Francisco J Ostos,Ana Serna-Gallego,María Trujillo-Rodríguez,Esperanza Muñoz-Muela,Teresa Giráldez-Pérez,Julia Praena-Segovia,María D Navarro-Amuedo,María Paniagua-García,Manuel García-Gutiérrez,Manuela Aguilar-Guisado,Inmaculada Rivas-Jeremías,María Reyes Jiménez-León,Sara Bachiller,Alberto Fernández-Villar,Alexandre Pérez-González,Alicia Gutiérrez-Valencia,Mohammed Rafii-El-Idrissi Benhnia,Daniela Weiskopf,Alessandro Sette,Luis F López-Cortés,Eva Poveda,Ezequiel Ruiz-MateosPubMed 35415890
- EMBO reports 23:e542712022Endothelial-immune crosstalk contributes to vasculopathy in nonalcoholic fatty liver disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChun-Yi Ng,Khang Leng Lee,Mark Dhinesh Muthiah,Kan Xing Wu,Florence Wen Jing Chioh,Konstanze Tan,Gwyneth Shook Ting Soon,Asim Shabbir,Wai Mun Loo,Zun Siong Low,Qingfeng Chen,Nguan Soon Tan,Huck Hui Ng,Yock Young Dan,Christine CheungPubMed 35403791
- Cellular & molecular immunology 18:2128-21392021Dendritic cell deficiencies persist seven months after SARS-CoV-2 infection.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlberto Pérez-Gómez,Joana Vitallé,Carmen Gasca-Capote,Alicia Gutierrez-Valencia,María Trujillo-Rodriguez,Ana Serna-Gallego,Esperanza Muñoz-Muela,María de Los Reyes Jiménez-Leon,Mohamed Rafii-El-Idrissi Benhnia,Inmaculada Rivas-Jeremias,Cesar Sotomayor,Cristina Roca-Oporto,Nuria Espinosa,Carmen Infante-Domínguez,Juan Carlos Crespo-Rivas,Alberto Fernández-Villar,Alexandre Pérez-González,Luis Fernando López-Cortés,Eva Poveda,Ezequiel Ruiz-MateosPubMed 34290398
- eLife 10:2021CRISPR-based functional genomics in human dendritic cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarco Jost,Amy N Jacobson,Jeffrey A Hussmann,Giana Cirolia,Michael A Fischbach,Jonathan S WeissmanPubMed 33904395
- Scientific reports 10:213602020Targeting macrophage priming by polyphyllin VII triggers anti-tumor immunity via STING-governed cytotoxic T-cell infiltration in lung cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJinglu Yu et. al.PubMed 33288772
- Molecular medicine (Cambridge, Mass.) 26:972020IP-10 and MCP-1 as biomarkers associated with disease severity of COVID-19.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYu Chen et. al.PubMed 33121429
- The Journal of infectious diseases 221:647-6592019Activation of C-Type Lectin Receptor and (RIG)-I-Like Receptors Contributes to Proinflammatory Response in Middle East Respiratory Syndrome Coronavirus-Infected Macrophages.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaoyu Zhao,Hin Chu,Bosco Ho-Yin Wong,Man Chun Chiu,Dong Wang,Cun Li,Xiaojuan Liu,Dong Yang,Vincent Kwok-Man Poon,Jianpiao Cai,Jasper Fuk-Woo Chan,Kelvin Kai-Wang To,Jie Zhou,Kwok-Yung YuenPubMed 31562757
Key facts
- Detection method
Colorimetric
- Sample types
Cell culture supernatant, Citrate plasma, EDTA Plasma, Heparin Plasma, Serum
- Assay type
Sandwich (quantitative)
- Reactive species
Human
- Range
12.5 - 800 pg/mL
- Assay time
1h 30m
- Sensitivity
= 2.6 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
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Target data
Function
Pro-inflammatory cytokine that is involved in a wide variety of processes such as chemotaxis, differentiation, and activation of peripheral immune cells, regulation of cell growth, apoptosis and modulation of angiostatic effects (PubMed:7540647, PubMed:11157474, PubMed:22652417). Plays thereby an important role during viral infections by stimulating the activation and migration of immune cells to the infected sites (By similarity). Mechanistically, binding of CXCL10 to the CXCR3 receptor activates G protein-mediated signaling and results in downstream activation of phospholipase C-dependent pathway, an increase in intracellular calcium production and actin reorganization (PubMed:12750173, PubMed:19151743). In turn, recruitment of activated Th1 lymphocytes occurs at sites of inflammation (PubMed:12750173, PubMed:12663757). Activation of the CXCL10/CXCR3 axis plays also an important role in neurons in response to brain injury for activating microglia, the resident macrophage population of the central nervous system, and directing them to the lesion site. This recruitment is an essential element for neuronal reorganization (By similarity).
Alternative names
C-X-C motif chemokine 10, 10 kDa interferon gamma-induced protein, Small-inducible cytokine B10, Gamma-IP10, IP-10, CXCL10, INP10, SCYB10
What's included?
Recommended products
Human IP-10 ELISA Kit ab173194 is a single-wash 90-min SimpleStep ELISA used to quantify Human IP-10 with a sensitivity of 2.6 pg/ml. The assay uses a simple Mix-Wash-Read protocol with just one incubation and wash step.
Easy-to-use, rapid, sensitive Human IP-10 sandwich ELISA Kit:
- Mix, Wash, Read: get results in 90 minutes with SimpleStep ELISA® format
- Colorimetric assay - 450nm readout; works on any plate reader
Key facts
- Detection method
Colorimetric
- Sample types
Cell culture supernatant, Citrate plasma, EDTA Plasma, Heparin Plasma, Serum
- Assay type
Sandwich (quantitative)
- Reactive species
Human
- Range
12.5 - 800 pg/mL
- Assay time
1h 30m
- Assay Platform
Microplate
- Sensitivity
= 2.6 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample PBMC media | n 9 | mean - | SD - | C.V. 5.1 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample PBMC media | n 3 | mean - | SD - | C.V. 11.1 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 103 | Range 102 - 105 % |
Sample type EDTA Plasma | Average % = 101 | Range 99 - 103 % |
Sample type Heparin Plasma | Average % = 86 | Range 82 - 88 % |
Sample type Citrate plasma | Average % = 96 | Range 93 - 98 % |
Sample type Cell culture media | Average % = 96 | Range 95 - 98 % |
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
+4°C
Notes
Human IP-10 (CXCL10) ELISA (ab173194) kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IP-10 protein in human serum and plasma samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate human IP-10 with 1.4 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpeStep ELISA® kits.
C-X-C motif chemokine 10 (CXCL10 or IP-10) is a small 10.8kD protein that is secreted by several cell types in response to interferon-gamma (IFNg). These cell types include monocytes, endothelial cells and fibroblasts. Upon secretion, CXCL10 is cleaved into an 8.7kD biologically active protein to function in chemotaxis for T-cells, NK cells, monocytes/macrophages and dendritic cells. In addition, CXCL10 has antitumor activity through the inhibition of bone marrow colony formation and angiogenesis. CXCL10 elicits its effects by binding to the cell surface chemokine receptor 3 (CXCR3).
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
IP10 also known as CXCL10 is a small cytokine belonging to the CXC chemokine family. It has a molecular mass of approximately 8.7 kDa. IP10 is secreted by several cell types such as monocytes endothelial cells and fibroblasts in response to interferon-gamma (IFN-γ). This protein is involved in immune responses and exhibits various roles especially in chemoattracting cells. Researchers often measure IP10 concentrations using ELISA kits such as the IP-10 ELISA to study its expression levels in different biological contexts.
- Biological function summary
IP10 plays a role in modulating the activities of immune cells. It attracts T cells eosinophils monocytes and natural killer (NK) cells by binding to the CXCR3 receptor. IP10 is not part of a larger complex but interacts with other cytokines to influence cell migration and the immune response. High levels of IP10 can reflect strong immune activation which is why it is often measured in inflammatory conditions using standard assays like the IP-10 ELISA kits.
- Pathways
The role of IP10 lies within the Th1-type immune response pathway. In this pathway IP10 works alongside other chemokines to recruit and activate immune cells to sites of inflammation or infection. It synergizes with IFN-γ to propagate immune signals. IP10 is also linked with the CXCR3 receptor which plays a critical role in these pathways providing a connection to other proteins such as CXCL9 and CXCL11 which have similar functions in cell-mediated immunity.
- Associated diseases and disorders
IP10 is associated with conditions like multiple sclerosis and rheumatoid arthritis. Elevated IP10 levels often correlate with disease activity in these disorders making it a potential biomarker for disease progression. The protein interacts with other inflammatory mediators such as TNF-α in regulating immune activity within these disease contexts. IP10's involvement in recruiting immune cells contributes to the pathogenic inflammation observed in these conditions.
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4 product images
Sandwich ELISA - Human IP-10 ELISA Kit (ab173194)
Example of IP-10 beta standard curve prepared in Sample Diluent NS.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human IP-10 ELISA Kit (ab173194)
Wild-type A549 control cells or IP-10 knockout A549 cells (Human CXCL10 (IP10) knockout A549 cell line ab266969), grown to 40% confluency, were stimulated with Recombinant Human Interferon gamma protein (Recombinant Human Interferon gamma protein (Active) ab259377) at 100 ng/ml and Recombinant human TNF alpha protein (Recombinant human TNF alpha protein (Active) ab259410) at 10 ng/ml or vehicle control for 16 or 32 hours.
THP-1 cells, grown to 40% confluency, were stimulated with Recombinant Human Interferon gamma protein (Recombinant Human Interferon gamma protein (Active) ab259377) at 200 ng/ml and LPS at 50 ng/mL or vehicle control for 24 hours.
The concentrations of IP-10 (CXCL10) in cell culture supernatants were measured in duplicate and interpolated from the IP-10 standard curves. IP-10 from vehicle control samples were measured in undiluted supernatants and the treated samples were diluted 200 times. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
Sandwich ELISA - Human IP-10 ELISA Kit (ab173194)
Typical ELISA data.
Titration of PBMC conditioned media (+/- PHA) within the working range of the assay. Background subtracted data from triplicate measurements are plotted.
This image is courtesy of an anonymous customer reviewSandwich ELISA - Human IP-10 ELISA Kit (ab173194)
Data shows specific secrection of IP-10 (CXCL10) by human macrophages differentiated in culture for 3 days in a dose response to M1 (MCSF + IFNg).
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