JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB325908

Human IP-10 ELISA Kit, Chemiluminescent

Be the first to review this product! Submit a review

|

(0 Publication)

Human IP-10 ELISA Kit, Chemiluminescent is a 55-min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Human IP-10 with higher sensitivity and broader measuring range.

- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 1.14 - 2500 pg/mL with a sensitivity of 0.62 pg/mL.
- Patent pending

View Alternative Names

INP10, SCYB10, CXCL10, C-X-C motif chemokine 10, 10 kDa interferon gamma-induced protein, Small-inducible cytokine B10, Gamma-IP10, IP-10

7 Images
Sandwich ELISA - Human IP-10 ELISA Kit, Chemiluminescent (AB325908)
  • sELISA

Lab

Sandwich ELISA - Human IP-10 ELISA Kit, Chemiluminescent (AB325908)

SimpleStep ELISA® Ignite technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 55 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

Sandwich ELISA - Human IP-10 ELISA Kit, Chemiluminescent (AB325908)
  • sELISA

Lab

Sandwich ELISA - Human IP-10 ELISA Kit, Chemiluminescent (AB325908)

Human peripheral blood cells (1x10^6 cells/mL) were cultured in RPMI media supplemented with 10% fetal calf serum, 100 U/mL penicillin, and 100 µg/mL streptomycin sulfate. Cells were cultured in the presence of absence of 10 µg/mL PHA. Conditioned media was harvested after 48 hours, aliquoted and assayed for endogenous IP-10 levels. The mean concentration of IP-10 was determined to be 996 pg/mL in unstimulated PBMC supernatant and 9,096 pg/mL in PHA stimulated supernatant.

Sandwich ELISA - Human IP-10 ELISA Kit, Chemiluminescent (AB325908)
  • sELISA

Lab

Sandwich ELISA - Human IP-10 ELISA Kit, Chemiluminescent (AB325908)

Standard curve comparison between the Chemiluminescent Human IP-10 SimpleStep ELISA and colorimetric Human IP-10 SimpleStep ELISA (ab173194) in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human IP-10 ELISA Kit, Chemiluminescent (AB325908)
  • sELISA

Lab

Sandwich ELISA - Human IP-10 ELISA Kit, Chemiluminescent (AB325908)

Dynamic range comparison between the Chemiluminescent Human IP-10 SimpleStep ELISA, colorimetric Human IP-10 SimpleStep ELISA (ab173194), and leading colorimetric competitor. The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and increased dynamic range.

Sandwich ELISA - Human IP-10 ELISA Kit, Chemiluminescent (AB325908)
  • sELISA

Supplier Data

Sandwich ELISA - Human IP-10 ELISA Kit, Chemiluminescent (AB325908)

Human peripheral blood cells (1x10^6 cells/mL) were cultured in RPMI media supplemented with 10% fetal calf serum, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. Cells were cultured in the presence of absence of 10 μg/mL PHA. Conditioned media was harvested after 48 hours, aliquoted and assayed for endogenous IP-10 levels. Titration of PBMC conditioned media (+/- PHA) within the working range of the assay. Background subtracted data from triplicate measurements are plotted.

Sandwich ELISA - Human IP-10 ELISA Kit, Chemiluminescent (AB325908)
  • sELISA

Lab

Sandwich ELISA - Human IP-10 ELISA Kit, Chemiluminescent (AB325908)

Wild-type A549 control cells or IP-10 knockout A549 cells (ab266969), grown to 40% confluency, were stimulated with Recombinant Human Interferon gamma protein (ab259377) at 100 ng/ml and Recombinant human TNF alpha protein (ab259410) at 10 ng/ml or vehicle control for 16 or 32 hours.

THP-1 cells, grown to 40% confluency, were stimulated with Recombinant Human Interferon gamma protein (ab259377) at 200 ng/ml and LPS at 50 ng/mL or vehicle control for 24 hours.

The concentrations of IP-10 (CXCL10) in cell culture supernatants were measured in duplicate and interpolated from the IP-10 standard curves. IP-10 from vehicle control samples were measured in undiluted supernatants and the treated samples were diluted 200 times. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Sandwich ELISA - Human IP-10 ELISA Kit, Chemiluminescent (AB325908)
  • sELISA

AbReview40601****

Sandwich ELISA - Human IP-10 ELISA Kit, Chemiluminescent (AB325908)

Data shows specific secrection of IP-10 (CXCL10) by human macrophages differentiated in culture for 3 days in a dose response to M1 (MCSF + IFNg).

This image is courtesy of an anonymous Abreview

Key facts

Detection method

Luminescent

Sample types

Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.62 pg/mL

Range

1.14 - 2500 pg/mL

Assay time

55m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Human IP-10 ELISA Kit, Chemiluminescent (ab325908) is a rapid single-wash sandwich 55-min ELISA to measure Human IP-10 in Serum, Plasma – Citrate, Plasma – EDTA, Plasma – Heparin, Cell Culture Supernatant with a sensitivity of 0.62 pg/mL.

How the assay works

Human IP-10 ELISA Kit, Chemiluminescent (ab325908) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.

Assay Specificity

Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human IP-10 (ab325908) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection
style
left-column

Precision

[ { "reproducibilityType": "Intra", "sample": "Supernatant", "replicates": 9, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 5.1" }, { "reproducibilityType": "Inter", "sample": "Supernatant", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 11.1" } ]
style
left-column

Recovery

[ { "sample": "Serum", "range": "102 - 105 %", "average": "= 103" }, { "sample": "Citrate plasma", "range": "93 - 98 %", "average": "= 96" }, { "sample": "EDTA Plasma", "range": "99 - 103 %", "average": "= 101" }, { "sample": "Heparin Plasma", "range": "82 - 88 %", "average": "= 86" }, { "sample": "Cell culture supernatant", "range": "95 - 98 %", "average": "= 96" } ]
style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab325908-1x96Tests", "assetComponentDetails": [ { "size":"1 x 600 µL", "name":"10X Human IP-10 Capture Antibody", "number":"AB325908-CMP01", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB325908-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human IP-10 Lyophilized Recombinant Protein", "number":"AB325908-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human IP-10 Detector Antibody", "number":"AB325908-CMP02", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB325908-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB325908-CMP09", "productcode":"" }, { "size":"1 x 3 mL", "name":"ChemiHRP Reagent A", "number":"AB325908-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated Black 96-Well Microplate", "number":"AB325908-CMP10", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB325908-CMP05", "productcode":"" }, { "size":"1 x 3 mL", "name":"ChemiHRP Reagent B", "number":"AB325908-CMP08", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
style
left-column
style
left-column

Product protocols

style
left-column

Target data

Pro-inflammatory cytokine that is involved in a wide variety of processes such as chemotaxis, differentiation, and activation of peripheral immune cells, regulation of cell growth, apoptosis and modulation of angiostatic effects (PubMed : 11157474, PubMed : 22652417, PubMed : 7540647). Plays thereby an important role during viral infections by stimulating the activation and migration of immune cells to the infected sites (By similarity). Mechanistically, binding of CXCL10 to the CXCR3 receptor activates G protein-mediated signaling and results in downstream activation of phospholipase C-dependent pathway, an increase in intracellular calcium production and actin reorganization (PubMed : 12750173, PubMed : 19151743). In turn, recruitment of activated Th1 lymphocytes occurs at sites of inflammation (PubMed : 12663757, PubMed : 12750173). Activation of the CXCL10/CXCR3 axis also plays an important role in neurons in response to brain injury for activating microglia, the resident macrophage population of the central nervous system, and directing them to the lesion site. This recruitment is an essential element for neuronal reorganization (By similarity).
See full target information CXCL10
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com