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AB83700

Human IP-10 ELISA Kit (CXCL10)

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(10 Publications)

Human IP-10 ELISA Kit (CXCL10) is a Sandwich (quantitative) ELISA for the measurement of Human IP-10 (CXCL10) in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

INP10, SCYB10, CXCL10, C-X-C motif chemokine 10, 10 kDa interferon gamma-induced protein, Small-inducible cytokine B10, Gamma-IP10, IP-10

1 Images
Sandwich ELISA - Human IP-10 ELISA Kit (CXCL10) (AB83700)
  • sELISA

Unknown

Sandwich ELISA - Human IP-10 ELISA Kit (CXCL10) (AB83700)

Representative Standard Curve using ab83700

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

< 5.7 pg/mL

Range

6.25 - 200 pg/mL

Assay time

3h 45m

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's IP-10 (CXCL10) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IP-10 in supernatants, buffered solutions, serum and plasma samples.

A monoclonal antibody specific for IP-10 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IP-10 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IP-10 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IP-10 present in the samples.

This kit will recognize both endogenous and recombinant Human IP-10.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Sample A", "replicates": 18, "mean": "56.9 pg/mL", "standardDeviation": "2.7", "coefficientOfVariability": "4.7" }, { "reproducibilityType": "Inter", "sample": "Sample B", "replicates": 18, "mean": "29.9 pg/mL", "standardDeviation": "2", "coefficientOfVariability": "6.5" }, { "reproducibilityType": "Inter", "sample": "Sample C", "replicates": 18, "mean": "17.7 pg/mL", "standardDeviation": "1.5", "coefficientOfVariability": "8.5" }, { "reproducibilityType": "Intra", "sample": "Sample A", "replicates": 6, "mean": "60.1 pg/mL", "standardDeviation": "0.9", "coefficientOfVariability": "1.6" }, { "reproducibilityType": "Intra", "sample": "Sample B", "replicates": 6, "mean": "32.4 pg/mL", "standardDeviation": "0.5", "coefficientOfVariability": "1.5" }, { "reproducibilityType": "Intra", "sample": "Sample C", "replicates": 6, "mean": "19.6 pg/mL", "standardDeviation": "0.3", "coefficientOfVariability": "1.3" } ]
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Recovery

[ { "sample": "Serum", "range": "118.7 - 122.4 %", "average": "= 120" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

IP10 also known as CXCL10 is a small cytokine belonging to the CXC chemokine family. It has a molecular mass of approximately 8.7 kDa. IP10 is secreted by several cell types such as monocytes endothelial cells and fibroblasts in response to interferon-gamma (IFN-γ). This protein is involved in immune responses and exhibits various roles especially in chemoattracting cells. Researchers often measure IP10 concentrations using ELISA kits such as the IP-10 ELISA to study its expression levels in different biological contexts.
Biological function summary

IP10 plays a role in modulating the activities of immune cells. It attracts T cells eosinophils monocytes and natural killer (NK) cells by binding to the CXCR3 receptor. IP10 is not part of a larger complex but interacts with other cytokines to influence cell migration and the immune response. High levels of IP10 can reflect strong immune activation which is why it is often measured in inflammatory conditions using standard assays like the IP-10 ELISA kits.

Pathways

The role of IP10 lies within the Th1-type immune response pathway. In this pathway IP10 works alongside other chemokines to recruit and activate immune cells to sites of inflammation or infection. It synergizes with IFN-γ to propagate immune signals. IP10 is also linked with the CXCR3 receptor which plays a critical role in these pathways providing a connection to other proteins such as CXCL9 and CXCL11 which have similar functions in cell-mediated immunity.

IP10 is associated with conditions like multiple sclerosis and rheumatoid arthritis. Elevated IP10 levels often correlate with disease activity in these disorders making it a potential biomarker for disease progression. The protein interacts with other inflammatory mediators such as TNF-α in regulating immune activity within these disease contexts. IP10's involvement in recruiting immune cells contributes to the pathogenic inflammation observed in these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Pro-inflammatory cytokine that is involved in a wide variety of processes such as chemotaxis, differentiation, and activation of peripheral immune cells, regulation of cell growth, apoptosis and modulation of angiostatic effects (PubMed : 11157474, PubMed : 22652417, PubMed : 7540647). Plays thereby an important role during viral infections by stimulating the activation and migration of immune cells to the infected sites (By similarity). Mechanistically, binding of CXCL10 to the CXCR3 receptor activates G protein-mediated signaling and results in downstream activation of phospholipase C-dependent pathway, an increase in intracellular calcium production and actin reorganization (PubMed : 12750173, PubMed : 19151743). In turn, recruitment of activated Th1 lymphocytes occurs at sites of inflammation (PubMed : 12663757, PubMed : 12750173). Activation of the CXCL10/CXCR3 axis also plays an important role in neurons in response to brain injury for activating microglia, the resident macrophage population of the central nervous system, and directing them to the lesion site. This recruitment is an essential element for neuronal reorganization (By similarity).
See full target information CXCL10
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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Oncology letters 25:225 PubMed37153045

2023

Study on the mechanism of oral administration of tetrandrine during neoadjuvant chemotherapy for colon cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Dan Li,Junmei Li,Feng Yu,Bo Wang,Bing Liu

Gut microbes 15:2197836 PubMed37017266

2023

promotes esophageal squamous cell carcinoma progression and chemoresistance by enhancing the secretion of chemotherapy-induced senescence-associated secretory phenotype via activation of DNA damage response pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jian-Wei Zhang,Dan Zhang,Hai-Sen Yin,Han Zhang,Kun-Qiao Hong,Jing-Ping Yuan,Bao-Ping Yu

Frontiers in immunology 13:879575 PubMed35572503

2022

H1N1 Influenza Virus-Infected Nasal Mucosal Epithelial Progenitor Cells Promote Dendritic Cell Recruitment and Maturation.

Applications

Unspecified application

Species

Unspecified reactive species

Fangyuan Zhu,Zhenxiao Teng,Xuanchen Zhou,Runtong Xu,Xin Bing,Lei Shi,Na Guo,Min Wang,Chengcheng Liu,Ming Xia

Archives of dermatological research 314:275-284 PubMed33866437

2021

Systemic CXCL10 is a predictive biomarker of vitiligo lesional skin infiltration, PUVA, NB-UVB and corticosteroid treatment response and outcome.

Applications

Unspecified application

Species

Unspecified reactive species

M El-Domyati,W H El-Din,A F Rezk,I Chervoneva,J B Lee,M Farber,J Uitto,O Igoucheva,Vitali Alexeev

Antioxidants (Basel, Switzerland) 9: PubMed33187129

2020

Hemin Prevents Increased Glycolysis in Macrophages upon Activation: Protection by Microbiota-Derived Metabolites of Polyphenols.

Applications

Unspecified application

Species

Unspecified reactive species

Catalina Carrasco-Pozo,Kah Ni Tan,Vicky M Avery

Journal of extracellular vesicles 9:1801153 PubMed32944190

2020

(Sub)populations of extracellular vesicles released by TNF-α -triggered human endothelial cells promote vascular inflammation and monocyte migration.

Applications

Unspecified application

Species

Unspecified reactive species

Baharak Hosseinkhani,Nynke M S van den Akker,Daniel G M Molin,Luc Michiels

Medicine 99:e20944 PubMed32629699

2020

Inhibition of ITGB1 enhance the anti-tumor effect of cetuximab in colorectal cancer cell.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaohui Yang,Shuai Wang,Weihua Yu,Yixiong Zheng,Yulian Wu

Frontiers in immunology 9:1789 PubMed30131806

2018

Extracellular Vesicles Work as a Functional Inflammatory Mediator Between Vascular Endothelial Cells and Immune Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Baharak Hosseinkhani,Sören Kuypers,Nynke M S van den Akker,Daniel G M Molin,Luc Michiels

Molecular medicine reports 17:6784-6788 PubMed29532873

2018

COMMD7 activates CXCL10 production by regulating NF-κB and the production of reactive oxygen species.

Applications

Unspecified application

Species

Unspecified reactive species

Nan You,Jing Li,Xiaobing Huang,Ke Wu,Yichen Tang,Liang Wang,Hongyan Li,Na Mi,Lu Zheng

Microbial pathogenesis 117:148-152 PubMed29432913

2018

Effects of fluconazole on the clinical outcome and immune response in fungal co-infected tuberculosis patients.

Applications

ELISA

Species

Unspecified reactive species

Xiaojuan Ren,Wei Liu,Yi Liu
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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