Human IP-10 ELISA Kit (CXCL10) is a Sandwich (quantitative) ELISA kit for the measurement of Human IP-10 (CXCL10) in Human in Plasma, Cell culture supernatant, Serum samples.
Colorimetric
Plasma, Cell culture supernatant, Serum
Sandwich (quantitative)
Human
6.25 - 200 pg/mL
3h 45m
< 5.7 pg/mL
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Pro-inflammatory cytokine that is involved in a wide variety of processes such as chemotaxis, differentiation, and activation of peripheral immune cells, regulation of cell growth, apoptosis and modulation of angiostatic effects (PubMed:11157474, PubMed:22652417, PubMed:7540647). Plays thereby an important role during viral infections by stimulating the activation and migration of immune cells to the infected sites (By similarity). Mechanistically, binding of CXCL10 to the CXCR3 receptor activates G protein-mediated signaling and results in downstream activation of phospholipase C-dependent pathway, an increase in intracellular calcium production and actin reorganization (PubMed:12750173, PubMed:19151743). In turn, recruitment of activated Th1 lymphocytes occurs at sites of inflammation (PubMed:12663757, PubMed:12750173). Activation of the CXCL10/CXCR3 axis also plays an important role in neurons in response to brain injury for activating microglia, the resident macrophage population of the central nervous system, and directing them to the lesion site. This recruitment is an essential element for neuronal reorganization (By similarity).
INP10, SCYB10, CXCL10, INP10, SCYB10, C-X-C motif chemokine 10, 10 kDa interferon gamma-induced protein, Small-inducible cytokine B10, Gamma-IP10, IP-10
Human IP-10 ELISA Kit (CXCL10) is a Sandwich (quantitative) ELISA kit for the measurement of Human IP-10 (CXCL10) in Human in Plasma, Cell culture supernatant, Serum samples.
Colorimetric
Plasma, Cell culture supernatant, Serum
Sandwich (quantitative)
Human
6.25 - 200 pg/mL
3h 45m
Microplate
< 5.7 pg/mL
Sample | n | mean | SD | C.V. |
---|---|---|---|---|
Sample Sample A | n 6 | mean 60.1 pg/mL | SD 0.9 | C.V. 1.6 |
Sample Sample B | n 6 | mean 32.4 pg/mL | SD 0.5 | C.V. 1.5 |
Sample Sample C | n 6 | mean 19.6 pg/mL | SD 0.3 | C.V. 1.3 |
Sample | n | mean | SD | C.V. |
---|---|---|---|---|
Sample Sample A | n 18 | mean 56.9 pg/mL | SD 2.7 | C.V. 4.7 |
Sample Sample B | n 18 | mean 29.9 pg/mL | SD 2 | C.V. 6.5 |
Sample Sample C | n 18 | mean 17.7 pg/mL | SD 1.5 | C.V. 8.5 |
Sample type | Average % | Range |
---|---|---|
Sample type Serum | Average % = 120 | Range 118.7 - 122.4 % |
Blue Ice
+4°C
+4°C
+4°C
Abcam's IP-10 (CXCL10) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IP-10 in supernatants, buffered solutions, serum and plasma samples.
A monoclonal antibody specific for IP-10 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IP-10 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IP-10 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IP-10 present in the samples.
This kit will recognize both endogenous and recombinant Human IP-10.
This supplementary information is collated from multiple sources and compiled automatically.
IP10 also known as CXCL10 is a small cytokine belonging to the CXC chemokine family. It has a molecular mass of approximately 8.7 kDa. IP10 is secreted by several cell types such as monocytes endothelial cells and fibroblasts in response to interferon-gamma (IFN-γ). This protein is involved in immune responses and exhibits various roles especially in chemoattracting cells. Researchers often measure IP10 concentrations using ELISA kits such as the IP-10 ELISA to study its expression levels in different biological contexts.
IP10 plays a role in modulating the activities of immune cells. It attracts T cells eosinophils monocytes and natural killer (NK) cells by binding to the CXCR3 receptor. IP10 is not part of a larger complex but interacts with other cytokines to influence cell migration and the immune response. High levels of IP10 can reflect strong immune activation which is why it is often measured in inflammatory conditions using standard assays like the IP-10 ELISA kits.
The role of IP10 lies within the Th1-type immune response pathway. In this pathway IP10 works alongside other chemokines to recruit and activate immune cells to sites of inflammation or infection. It synergizes with IFN-γ to propagate immune signals. IP10 is also linked with the CXCR3 receptor which plays a critical role in these pathways providing a connection to other proteins such as CXCL9 and CXCL11 which have similar functions in cell-mediated immunity.
IP10 is associated with conditions like multiple sclerosis and rheumatoid arthritis. Elevated IP10 levels often correlate with disease activity in these disorders making it a potential biomarker for disease progression. The protein interacts with other inflammatory mediators such as TNF-α in regulating immune activity within these disease contexts. IP10's involvement in recruiting immune cells contributes to the pathogenic inflammation observed in these conditions.
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