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AB316903

Human IP-10 ELISA Kit, Fluorescent

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Human IP-10 ELISA Kit, Fluorescent is a single-wash 90-min Simplestep used to quantify Human IP-10 with a sensitivity of 1.039 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Fluorescent Sandwich ELISA - 530/570/590 nm readout : works on any standard plate reader

View Alternative Names

INP10, SCYB10, CXCL10, C-X-C motif chemokine 10, 10 kDa interferon gamma-induced protein, Small-inducible cytokine B10, Gamma-IP10, IP-10

5 Images
Sandwich ELISA - Human IP-10 ELISA Kit, Fluorescent (AB316903)
  • sELISA

Supplier Data

Sandwich ELISA - Human IP-10 ELISA Kit, Fluorescent (AB316903)

Interpolated concentration of native IP-10 was measured in duplicate at different sample concentrations. Undiluted samples are as follows : plasma (EDTA) 100% and plasma (heparin) 100%.  The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human IP-10 ELISA Kit, Fluorescent (AB316903)
  • sELISA

Supplier Data

Sandwich ELISA - Human IP-10 ELISA Kit, Fluorescent (AB316903)

Example of human IP-10 standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human IP-10 ELISA Kit, Fluorescent (AB316903)
  • sELISA

Supplier Data

Sandwich ELISA - Human IP-10 ELISA Kit, Fluorescent (AB316903)

Standard curve comparison between the original Human IP-10 SimpleStep ELISA (ab173194) and current Human IP-10 SimpleStep ELISA (ab289906).

Sandwich ELISA - Human IP-10 ELISA Kit, Fluorescent (AB316903)
  • sELISA

Supplier Data

Sandwich ELISA - Human IP-10 ELISA Kit, Fluorescent (AB316903)

Interpolated concentration of native IP-10 was measured in duplicate at different sample concentrations. Undiluted samples are THP-1 supernatant 0.25%.  The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human IP-10 ELISA Kit, Fluorescent (AB316903)
  • sELISA

Supplier Data

Sandwich ELISA - Human IP-10 ELISA Kit, Fluorescent (AB316903)

Standard curve comparison between Human IP-10 SimpleStep ELISA kit (ab289906) and traditional ELISA kit from leading competitor. The SimpleStep ELISA kit shows increased sensitivity.

Key facts

Detection method

Fluorescent

Sample types

EDTA Plasma, Heparin Plasma, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 1.039 pg/mL

Range

3.125 - 200 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human IP-10 SimpleStep ELISA® kit, fluorescent is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IP-10 protein in EDTA Plasma, Hep Plasma, Cell culture supernatant. Quantitate Human IP-10 with 1.039 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Supernatant", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "8.2" }, { "reproducibilityType": "Inter", "sample": "Supernatant", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "8.3" } ]
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Recovery

[ { "sample": "EDTA Plasma", "range": "81 - 87 %", "average": "= 84" }, { "sample": "Heparin Plasma", "range": "93 - 116 %", "average": "= 103" }, { "sample": "Cell culture supernatant", "range": "81 - 105 %", "average": "= 94" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab316903-1x96Tests", "assetComponentDetails": [ { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB316903-CMP06", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB316903-CMP04", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB316903-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human IP-10 Lyophilized Recombinant Protein", "number":"AB316903-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human IP-10 Capture Antibody", "number":"AB316903-CMP01", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated Black 96-Well Microplate", "number":"AB316903-CMP09", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human IP-10 Detector Antibody", "number":"AB316903-CMP02", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stoplight Red Substrate Buffer", "number":"AB316903-CMP07", "productcode":"" }, { "size":"1 x 120 µL", "name":"100X Stoplight Red Substrate", "number":"AB316903-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB316903-CMP10", "productcode":"" }, { "size":"1 x 50 µL", "name":"500X Hydrogen Peroxide (H2O2, 3%)", "number":"AB316903-CMP11", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

IP10 also known as CXCL10 is a small cytokine belonging to the CXC chemokine family. It has a molecular mass of approximately 8.7 kDa. IP10 is secreted by several cell types such as monocytes endothelial cells and fibroblasts in response to interferon-gamma (IFN-γ). This protein is involved in immune responses and exhibits various roles especially in chemoattracting cells. Researchers often measure IP10 concentrations using ELISA kits such as the IP-10 ELISA to study its expression levels in different biological contexts.
Biological function summary

IP10 plays a role in modulating the activities of immune cells. It attracts T cells eosinophils monocytes and natural killer (NK) cells by binding to the CXCR3 receptor. IP10 is not part of a larger complex but interacts with other cytokines to influence cell migration and the immune response. High levels of IP10 can reflect strong immune activation which is why it is often measured in inflammatory conditions using standard assays like the IP-10 ELISA kits.

Pathways

The role of IP10 lies within the Th1-type immune response pathway. In this pathway IP10 works alongside other chemokines to recruit and activate immune cells to sites of inflammation or infection. It synergizes with IFN-γ to propagate immune signals. IP10 is also linked with the CXCR3 receptor which plays a critical role in these pathways providing a connection to other proteins such as CXCL9 and CXCL11 which have similar functions in cell-mediated immunity.

IP10 is associated with conditions like multiple sclerosis and rheumatoid arthritis. Elevated IP10 levels often correlate with disease activity in these disorders making it a potential biomarker for disease progression. The protein interacts with other inflammatory mediators such as TNF-α in regulating immune activity within these disease contexts. IP10's involvement in recruiting immune cells contributes to the pathogenic inflammation observed in these conditions.
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Product protocols

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Target data

Pro-inflammatory cytokine that is involved in a wide variety of processes such as chemotaxis, differentiation, and activation of peripheral immune cells, regulation of cell growth, apoptosis and modulation of angiostatic effects (PubMed : 11157474, PubMed : 22652417, PubMed : 7540647). Plays thereby an important role during viral infections by stimulating the activation and migration of immune cells to the infected sites (By similarity). Mechanistically, binding of CXCL10 to the CXCR3 receptor activates G protein-mediated signaling and results in downstream activation of phospholipase C-dependent pathway, an increase in intracellular calcium production and actin reorganization (PubMed : 12750173, PubMed : 19151743). In turn, recruitment of activated Th1 lymphocytes occurs at sites of inflammation (PubMed : 12663757, PubMed : 12750173). Activation of the CXCL10/CXCR3 axis also plays an important role in neurons in response to brain injury for activating microglia, the resident macrophage population of the central nervous system, and directing them to the lesion site. This recruitment is an essential element for neuronal reorganization (By similarity).
See full target information CXCL10
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websiteProtocolBooklet
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