AB219630

Human IRAK-1 ELISA Kit

Recombinant
SimpleStep
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Human IRAK-1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human IRAK-1 with a sensitivity of 22 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

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Sandwich ELISA - Human IRAK-1 ELISA Kit (AB219630)

sELISA

Supplier Data

Sandwich ELISA - Human IRAK-1 ELISA Kit (AB219630)

Interpolated concentrations of native IRAK-1 in human MCF7, HEK293T, and K562 extracts based on a 500. 125 and 250 μg/mL extract load.

The concentrations of IRAK-1 were measured in duplicate and interpolated from the IRAK-1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IRAK-1 concentration was determined to be 8178 pg/mL in MCF7 extracts, 3768 pg/mL in HEK93T extracts, and 3927 pg/mL in K562 extracts.

sELISA

Supplier Data

Sandwich ELISA - Human IRAK-1 ELISA Kit (AB219630)

Representative image of Human IRAK-1 ELISA Kit (ab219630)

Components shown from left to right :

- 10X Wash Buffer PT (ab206977)

- 10X Human IRAK-1 ELISA Kit Capture Antibody

- 10X Human IRAK-1 Detector Antibody

- 10X Human IRAK-1 ELISA Kit Lyophilised Purified Protein (2 vials)

- Human IRAK-1 ELISA Kit 50X Cell Extraction Enhancer Solution (ab193971)

- Sample diluent NS

- TMB solution

- Stop Solution

- 5X Cell Extraction Buffer PTR (ab193970)

- Antibody Diluent 4BI

SimpleStep Pre-Coated 96-Well Microplate (ab206978) - shown behind bottles / vials.

Plate Seals - shown underneath bottles / vials.

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the ELISA kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit

sELISA

Lab

Sandwich ELISA - Human IRAK-1 ELISA Kit (AB219630)

Interpolated concentration of native IRAK-1 was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 125 µg/mL HEK293T cell extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

sELISA

Lab

Sandwich ELISA - Human IRAK-1 ELISA Kit (AB219630)

Example of human IRAK-1 standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human IRAK-1 ELISA Kit (AB219630)

Example of human IRAK-1 standard curve in 1X Cell Extraction Buffer PTR.

Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell culture extracts

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 22 pg/mL

Range

156 - 10000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human IRAK-1 ELISA Kit (ab219630) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IRAK-1 protein in cell culture extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human IRAK-1 with 22 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

IRAK-1 is a serine/threonine-protein kinase involved in the innate immune response against pathogens. IRAK-1's association with MYC88 after TLR activation leads to its phosphorylation by IRAK-4. IRAK-1 phosphorylation eventually results in the transcriptional activation of type 1 IFN genes.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.3" } ]

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Recovery

[ { "sample": "Cell culture extracts", "range": "107 - 113 %", "average": "= 110" } ]

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What's included?

{ "values": { "1x384Tests": { "sellingSize": "1 x 384 Tests", "publicAssetCode":"ab219630-1x384Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB219630-CMP11" }, { "size":"1 x 1 Unit", "name":"384 well CaptSure™ microplates", "number":"AB219630-CMP14" }, { "size":"6 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB219630-CMP07" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB219630-CMP09" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB219630-CMP12" }, { "size":"2 x 12 mL", "name":"Stop Solution", "number":"AB219630-CMP05" }, { "size":"2 x 12 mL", "name":"TMB Development Solution", "number":"AB219630-CMP06" }, { "size":"1 x 50 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB219630-CMP08" }, { "size":"1 x 600 µL", "name":"10X Human IRAK-1 Capture Antibody", "number":"AB219630-CMP02" }, { "size":"2 x 1 Vial", "name":"Human IRAK-1 Lyophilized Recombinant Protein", "number":"AB219630-CMP10" }, { "size":"1 x 600 µL", "name":"10X Human IRAK-1 Detector Antibody", "number":"AB219630-CMP03" } ] }, "10x96Tests": { "sellingSize": "10 x 96 Tests", "publicAssetCode":"ab219630-10x96Tests", "assetComponentDetails": [ { "size":"10 x 1 Unit", "name":"Plate Seal", "number":"AB219630-CMP11" }, { "size":"10 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB219630-CMP07" }, { "size":"10 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB219630-CMP04" }, { "size":"10 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB219630-CMP09" }, { "size":"1 x 200 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB219630-CMP12" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB219630-CMP05" }, { "size":"1 x 120 mL", "name":"TMB Development Solution", "number":"AB219630-CMP06" }, { "size":"2 x 50 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB219630-CMP08" }, { "size":"10 x 2 Vial", "name":"Human IRAK-1 Lyophilized Recombinant Protein", "number":"AB219630-CMP10" }, { "size":"1 x 600 µL", "name":"10X Human IRAK-1 Capture Antibody", "number":"AB219630-CMP02" }, { "size":"10 x 600 µL", "name":"10X Human IRAK-1 Detector Antibody", "number":"AB219630-CMP03" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab219630-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB219630-CMP11", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB219630-CMP08", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB219630-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB219630-CMP04", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB219630-CMP12", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB219630-CMP05", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB219630-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB219630-CMP13", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB219630-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human IRAK-1 Lyophilized Recombinant Protein", "number":"AB219630-CMP10", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human IRAK-1 Capture Antibody", "number":"AB219630-CMP02", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human IRAK-1 Detector Antibody", "number":"AB219630-CMP03", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

IRAK-1 also known as Interleukin-1 Receptor-Associated Kinase 1 is a serine/threonine kinase that plays a role in the immune response. With a molecular mass around 76 kDa this protein is heavily expressed in immune cells such as macrophages and monocytes. IRAK-1 is an important player in the signal transduction process following the activation of the Toll-like and interleukin-1 receptor (IL-1R) families. The kinase activity of IRAK-1 occurs when it associates with the receptor complex transmitting signals that activate downstream pathways.

Biological function summary

IRAK-1 mediates the inflammatory response by promoting the production of cytokines and other inflammatory mediators. It is a component of the Myddosome a multi-protein complex that assembles upon receptor activation and includes MyD88 an adaptor protein needed for signal transduction. IRAK-1 phosphorylates other targets and itself leading to its own degradation which is an important step for turning off the signaling and preventing prolonged activation. Its precise activity modulation ensures balanced immune responses and prevents overreaction that could cause tissue damage.

Pathways

IRAK-1 functions in the IL-1 and Toll-like receptor signaling pathways both of which are important for innate immunity. In these pathways IRAK-1 interacts with key proteins such as TRAF6 which transduces signals leading to NF-κB and MAPK activation critical for inflammatory gene expression. Its role in these pathways highlights the importance of proper function and regulation as deficiencies or dysregulation can result in inappropriate immune responses.

IRAK-1 has been connected to autoimmune diseases such as rheumatoid arthritis and certain cancers. This protein affects how the immune system responds to inflammation and can influence disease progression through its regulatory actions. For instance alterations in IRAK-1 activity or expression can lead to an increase in pro-inflammatory cytokines that exacerbate conditions like rheumatoid arthritis. Additionally in cancer pathways influenced by IRAK-1 can affect tumor growth and metastasis. Its interaction with proteins such as MyD88 and TRAF6 within these pathological contexts underlines its importance as a potential target for therapeutic intervention.

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Product protocols

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Target data

Serine/threonine-protein kinase that plays a critical role in initiating innate immune response against foreign pathogens. Involved in Toll-like receptor (TLR) and IL-1R signaling pathways. Is rapidly recruited by MYD88 to the receptor-signaling complex upon TLR activation. Association with MYD88 leads to IRAK1 phosphorylation by IRAK4 and subsequent autophosphorylation and kinase activation. Phosphorylates E3 ubiquitin ligases Pellino proteins (PELI1, PELI2 and PELI3) to promote pellino-mediated polyubiquitination of IRAK1. Then, the ubiquitin-binding domain of IKBKG/NEMO binds to polyubiquitinated IRAK1 bringing together the IRAK1-MAP3K7/TAK1-TRAF6 complex and the NEMO-IKKA-IKKB complex. In turn, MAP3K7/TAK1 activates IKKs (CHUK/IKKA and IKBKB/IKKB) leading to NF-kappa-B nuclear translocation and activation. Alternatively, phosphorylates TIRAP to promote its ubiquitination and subsequent degradation. Phosphorylates the interferon regulatory factor 7 (IRF7) to induce its activation and translocation to the nucleus, resulting in transcriptional activation of type I IFN genes, which drive the cell in an antiviral state. When sumoylated, translocates to the nucleus and phosphorylates STAT3.

See full target information IRAK1

Alternative Names

IRAK, IRAK1, Interleukin-1 receptor-associated kinase 1, IRAK-1

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Human IRAK-1 ELISA Kit

Sandwich ELISA - Human IRAK-1 ELISA Kit (AB219630)

Sandwich ELISA - Human IRAK-1 ELISA Kit (AB219630)

Sandwich ELISA - Human IRAK-1 ELISA Kit (AB219630)

Sandwich ELISA - Human IRAK-1 ELISA Kit (AB219630)

Sandwich ELISA - Human IRAK-1 ELISA Kit (AB219630)

Key facts

Detection method

Sample types

Reacts with

Assay type

Results type

Sensitivity

Range

Assay time

Assay Platform

Complementary Products

Assay Kits

Assay Kits

Assay Kits

Primary Antibodies

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Alternative Names

Product promise