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AB326391

Human IRAK-1 ELISA Kit, Chemiluminescent

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Human IRAK-1 ELISA Kit, Chemiluminescent is a 55-min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Human IRAK-1 with higher sensitivity and broader measuring range.

- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 18.29 - 40000 pg/ml with a sensitivity of 3.93pg/mL.
- Design your own immunoassay: we also offer the conjugation-ready antibody pair.
-Patent pending

View Alternative Names

IRAK, IRAK1, Interleukin-1 receptor-associated kinase 1, IRAK-1

4 Images
Sandwich ELISA - Human IRAK-1 ELISA Kit, Chemiluminescent (AB326391)
  • sELISA

Lab

Sandwich ELISA - Human IRAK-1 ELISA Kit, Chemiluminescent (AB326391)

SimpleStep ELISA® Ignite technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 55 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

Sandwich ELISA - Human IRAK-1 ELISA Kit, Chemiluminescent (AB326391)
  • sELISA

Lab

Sandwich ELISA - Human IRAK-1 ELISA Kit, Chemiluminescent (AB326391)

Dynamic range comparison between the Chemiluminescent Human IRAK-1 SimpleStep ELISA, colorimetric Human IRAK-1 SimpleStep ELISA (ab219630), and leading colorimetric competitor. The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and increased dynamic range.

Sandwich ELISA - Human IRAK-1 ELISA Kit, Chemiluminescent (AB326391)
  • sELISA

Lab

Sandwich ELISA - Human IRAK-1 ELISA Kit, Chemiluminescent (AB326391)

Standard curve comparison between the Chemiluminescent Human IRAK-1 SimpleStep ELISA and colorimetric Human IRAK-1 SimpleStep ELISA (ab219630) in 1X Cell Extraction Buffer PTR. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human IRAK-1 ELISA Kit, Chemiluminescent (AB326391)
  • sELISA

Supplier Data

Sandwich ELISA - Human IRAK-1 ELISA Kit, Chemiluminescent (AB326391)

Interpolated concentrations of native IRAK-1 in human MCF7 HEK293T and K562 extracts based on a 500. 125 and 250 µg/mL extract load.

The concentrations of IRAK-1 were measured in duplicate and interpolated from the IRAK-1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD n=2). The mean IRAK-1 concentration was determined to be 8178 pg/mL in MCF7 extracts 3768 pg/mL in HEK93T extracts and 3927 pg/mL in K562 extracts.

Key facts

Detection method

Luminescent

Sample types

Cell culture extracts

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 3.93 pg/mL

Range

18.29 - 40000 pg/mL

Assay time

55m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human IRAK-1 ELISA Kit, Chemiluminescent (ab326391) is a rapid single-wash sandwich 55-min ELISA to measure Human IRAK-1 in Cell culture extracts with a sensitivity of 3.93 pg/mL.

How the assay works

Human IRAK-1 ELISA Kit, Chemiluminescent (ab326391) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.

Assay Specificity

Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human IRAK-1 (ab326391) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-IRAK-1 antibody [EPR19667-168] - BSA and Azide free (Capture) ab242648
- Anti-IRAK-1 antibody [EPR19667-153] - BSA and Azide free (Detector) ab242893
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Precision

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 3.3" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 4" } ]
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Recovery

[ { "sample": "Cell culture extracts", "range": "107 - 113 %", "average": "= 110" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab326391-1x96Tests", "assetComponentDetails": [ { "size":"1 x 600 µL", "name":"10X Human IRAK-1 Capture Antibody", "number":"AB326391-CMP01", "productcode":"" }, { "size":"1 x 3 mL", "name":"ChemiHRP Reagent B", "number":"AB326391-CMP09", "productcode":"" }, { "size":"1 x 3 mL", "name":"ChemiHRP Reagent A", "number":"AB326391-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated Black 96-Well Microplate", "number":"AB326391-CMP10", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human IRAK-1 Detector Antibody", "number":"AB326391-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB326391-CMP11", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human IRAK-1 Lyophilized Recombinant Protein", "number":"AB326391-CMP03", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB326391-CMP06", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB326391-CMP05", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB326391-CMP07", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB326391-CMP04", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Serine/threonine-protein kinase that plays a critical role in initiating innate immune response against foreign pathogens. Involved in Toll-like receptor (TLR) and IL-1R signaling pathways. Is rapidly recruited by MYD88 to the receptor-signaling complex upon TLR activation. Association with MYD88 leads to IRAK1 phosphorylation by IRAK4 and subsequent autophosphorylation and kinase activation. Phosphorylates E3 ubiquitin ligases Pellino proteins (PELI1, PELI2 and PELI3) to promote pellino-mediated polyubiquitination of IRAK1. Then, the ubiquitin-binding domain of IKBKG/NEMO binds to polyubiquitinated IRAK1 bringing together the IRAK1-MAP3K7/TAK1-TRAF6 complex and the NEMO-IKKA-IKKB complex. In turn, MAP3K7/TAK1 activates IKKs (CHUK/IKKA and IKBKB/IKKB) leading to NF-kappa-B nuclear translocation and activation. Alternatively, phosphorylates TIRAP to promote its ubiquitination and subsequent degradation. Phosphorylates the interferon regulatory factor 7 (IRF7) to induce its activation and translocation to the nucleus, resulting in transcriptional activation of type I IFN genes, which drive the cell in an antiviral state. When sumoylated, translocates to the nucleus and phosphorylates STAT3.
See full target information IRAK1
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websiteProtocolBooklet
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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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