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AB288179

Human IRAK4 ELISA Kit, Fluorescent

  • CatchPoint SimpleStep ELISA
  • Recombinant
  • SimpleStep
  • What is this?

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Human IRAK4 ELISA Kit, Fluorescent is a single-wash 90-min Simplestep used to quantify Human IRAK4 with a sensitivity of 137 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Fluorescent Sandwich ELISA - 530/570/590 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Interleukin-1 receptor-associated kinase 4, IRAK-4, Renal carcinoma antigen NY-REN-64, IRAK4

3 Images
Sandwich ELISA - Human IRAK4 ELISA Kit, Fluorescent (AB288179)
  • sELISA

Supplier Data

Sandwich ELISA - Human IRAK4 ELISA Kit, Fluorescent (AB288179)

Example of human IRAK4 standard curve in 1X Cell Extraction Buffer PTR. Example human IRAK4 standard curve. Background-subtracted data values (mean =/- SD) are graphed.

Sandwich ELISA - Human IRAK4 ELISA Kit, Fluorescent (AB288179)
  • sELISA

Unknown

Sandwich ELISA - Human IRAK4 ELISA Kit, Fluorescent (AB288179)

Other - Human IRAK4 ELISA Kit, Fluorescent.

SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

Sandwich ELISA - Human IRAK4 ELISA Kit, Fluorescent (AB288179)
  • sELISA

Supplier Data

Sandwich ELISA - Human IRAK4 ELISA Kit, Fluorescent (AB288179)

Interpolated concentrations of human IRAK4 in human THP1 and PBMC extract.

The endogenous concentration of IRAK4 was measured in duplicate at different sample concentrations. Undiluted samples are 500 μg/mL human THP1 and PBMC extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

Key facts

Detection method

Fluorescent

Sample types

Cell culture extracts, Tissue Extracts

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 137 pg/mL

Range

0.25 - 64 ng/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Complementary Products

Assay Kits

AB218182

Human IRAK4 Matched Antibody Pair Kit

Recombinant

ELISA - Human IRAK4 Matched Antibody Pair Kit (AB218182)

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Primary Antibodies

AB300077

Anti-IRAK4 antibody [EPR24506-71]

20ul selling size|RabMAb|Recombinant|Trial Size

Immunoprecipitation - Anti-IRAK4 antibody [EPR24506-71] (AB300077)

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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

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Assay Kits

AB220136

Human IRAK-1 Matched Antibody Pair Kit

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ELISA - Human IRAK-1 Matched Antibody Pair Kit (AB220136)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human IRAK4 in vitro CatchPoint SimpleStep ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IRAK4 protein in plasma and serum samples.

This CatchPoint SimpleStep ELISA kit has been optimized for Molecular Devices Microplate Readers. Click here for a list of recommended Microplate Readers.
If using a Molecular Devices' plate reader supported by SoftMax® Pro software, a preconfigured protocol for these CatchPoint SimpleStep ELISA Kits is available with all the protocol and analysis settings at www.softmaxpro.org.

The CatchPoint SimpleStep ELISA employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. CatchPoint HRP Development Solution containing the Stoplight Red Substrate is added. During incubation, the substrate is catalyzed by HRP generating a fluorescent product. Signal is generated proportionally to the amount of bound analyte and the intensity is measured in a fluorescence plater reader at 530/570/590 nm Excitation/Cutoff/Emission.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5" } ]
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Recovery

[ { "sample": "Cell culture extracts", "range": "107 - 115 %", "average": "= 111" }, { "sample": "Tissue Extracts", "range": "100 - 101 %", "average": "= 101" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab288179-1x96Tests", "assetComponentDetails": [ { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB288179-CMP13", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB288179-CMP14", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB288179-CMP15", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB288179-CMP12", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human IRAK4 Capture Antibody", "number":"AB288179-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human IRAK4 Lyophilized Recombinant Protein", "number":"AB288179-CMP11", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BC", "number":"AB288179-CMP10", "productcode":"" }, { "size":"1 x 50 µL", "name":"500X Hydrogen Peroxide (H2O2, 3%)", "number":"AB288179-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (12 x 8 wells)", "number":"AB288179-CMP05", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human IRAK4 Detector Antibody", "number":"AB288179-CMP04", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB288179-CMP09", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stoplight Red Substrate Buffer", "number":"AB288179-CMP06", "productcode":"" }, { "size":"1 x 120 µL", "name":"100X Stoplight Red Substrate", "number":"AB288179-CMP01", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
Multi
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

IRAK4 also known as Interleukin-1 receptor-associated kinase 4 is an important protein with an approximate molecular mass of 51 kDa. This protein is a significant part of the signaling cascade initiated by the Interleukin-1 receptor (IL-1R) and Toll-like receptors (TLRs). Expressed in a variety of tissues including the spleen thymus and lung IRAK4 is involved in innate immune responses. The presence of IRAK4 protein facilitates the downstream signaling pathways that activate the transcription factors responsible for inflammatory cytokine production.
Biological function summary

IRAK4 plays an important role in mediating signaling in the innate immune system by forming complexes with other proteins such as IRAK-1. These complexes propagate signals that result in the activation of transcription factors like NF-kB and AP-1 which regulate the expression of inflammatory genes. Through these mechanisms IRAK4 influences the body's ability to respond to infections and stresses. Its critical involvement in these signaling pathways highlights its importance in maintaining immune homeostasis.

Pathways

IRAK4 is a pivotal player in the Toll-like receptor and IL-1 receptor signaling pathways which are central to the host defense against pathogens. It functions upstream activating the MyD88-dependent pathway resulting in the recruitment and phosphorylation of other kinases such as IRAK-1 and TRAF6. These interactions trigger the NF-kB signaling cascade thereby enhancing pro-inflammatory cytokine production that is essential for initiating immune responses.

Mutations or dysregulation of IRAK4 are linked to increased susceptibility to recurrent bacterial infections and sepsis. Patients with IRAK4 deficiency have impaired NF-kB activation leading to a weakened inflammatory response. Additionally overexpression of IRAK4 has been observed in certain autoimmune diseases such as rheumatoid arthritis where the persistent activation of TLR signaling contributes to chronic inflammation. The role of IRAK4 in these disorders ties closely with its interaction with proteins like TRAF6 influencing disease development and progression.
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Product protocols

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Target data

Serine/threonine-protein kinase that plays a critical role in initiating innate immune response against foreign pathogens. Involved in Toll-like receptor (TLR) and IL-1R signaling pathways (PubMed : 17878374). Is rapidly recruited by MYD88 to the receptor-signaling complex upon TLR activation to form the Myddosome together with IRAK2. Phosphorylates initially IRAK1, thus stimulating the kinase activity and intensive autophosphorylation of IRAK1. Phosphorylates E3 ubiquitin ligases Pellino proteins (PELI1, PELI2 and PELI3) to promote pellino-mediated polyubiquitination of IRAK1. Then, the ubiquitin-binding domain of IKBKG/NEMO binds to polyubiquitinated IRAK1 bringing together the IRAK1-MAP3K7/TAK1-TRAF6 complex and the NEMO-IKKA-IKKB complex. In turn, MAP3K7/TAK1 activates IKKs (CHUK/IKKA and IKBKB/IKKB) leading to NF-kappa-B nuclear translocation and activation. Alternatively, phosphorylates TIRAP to promote its ubiquitination and subsequent degradation. Phosphorylates NCF1 and regulates NADPH oxidase activation after LPS stimulation suggesting a similar mechanism during microbial infections.
See full target information IRAK4
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websiteProtocolBooklet
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Product promise

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