AB289646

Human IRS1 ELISA Kit

Recombinant
SimpleStep
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Human IRS1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human IRS1 with a sensitivity of 0.096 ng/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Insulin receptor substrate 1, IRS-1, IRS1

2 Images

Sandwich ELISA - Human IRS1 ELISA Kit (AB289646)

sELISA

Supplier Data

Sandwich ELISA - Human IRS1 ELISA Kit (AB289646)

Interpolated concentrations of human IRS1 in MCF-7 cell extract and Hela cell extract.

Interpolated concentration of native IRS1 was measured in duplicate at different sample concentrations. Undiluted samples are as follows : MCF-7 cell extract 100 μg/mL and Hela cell extract 500 μg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

sELISA

Supplier Data

Sandwich ELISA - Human IRS1 ELISA Kit (AB289646)

Example of human IRS1 standard curve in 1X Cell Extraction Buffer PTR.

Example of human IRS1 standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.096 ng/mL

Range

0.219 - 14 ng/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human IRS1 ELISA Kit (ab289646) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human IRS1 in cell extract samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human IRS1 with 0.096 ng/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 4, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5" }, { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.7" } ]

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Recovery

[ { "sample": "Cell Lysate", "range": "101 - 106 %", "average": "= 103" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab289646-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB289646-CMP06", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB289646-CMP15", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human IRS1 Lyophilized Recombinant Protein", "number":"AB289646-CMP10", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB289646-CMP12", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB289646-CMP14", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human IRS1 Capture Antibody", "number":"AB289646-CMP03", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB289646-CMP13", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human IRS1 Detector Antibody", "number":"AB289646-CMP04", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB289646-CMP09", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB289646-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB289646-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB289646-CMP11", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein IRS1 also known as Insulin Receptor Substrate 1 is an important player in insulin signaling. It is a docking protein that plays a part in the signaling pathway of insulin and insulin-like growth factor (IGF) contributing to glucose homeostasis. The molecular weight of IRS1 is approximately 180 kDa. Expressed in various tissues including muscle liver and adipose tissue IRS1 facilitates the transmission of signals from activated cell surface receptors to intracellular pathways. Notable for its phosphorylation IRS1 undergoes change in state when interacting with receptor kinases impacting its role in cell signaling.

Biological function summary

IRS1 helps mediate the effects of insulin by acting as a downstream effector in the insulin signaling cascade. IRS1 functions in a complex manner binding with PI3K (phosphoinositide 3-kinase) after phosphorylation at specific tyrosine residues. This interaction is important for further signaling events leading to the regulation of glucose uptake lipid synthesis and gene expression. Besides its role in energy balance IRS1 plays a significant part in cell growth and differentiation driven by its ability to relay signals from the insulin and IGF receptors.

Pathways

IRS1 is heavily involved in the insulin signaling pathway and the PI3K-Akt pathway. Upon phosphorylation IRS1 binds to proteins such as the PI3K initiating a cascade that ultimately activates Akt resulting in anabolic processes within cells. The pathway is essential for regulating basic functions like metabolism growth and survival. IRS1's interaction with other related proteins like IR (insulin receptor) and IGFR (insulin-like growth factor receptor) highlights its integrative role in cellular processes related to energy and growth.

IRS1 is implicated in insulin resistance and type 2 diabetes. Changes in IRS1 phosphorylation patterns can disrupt normal insulin signaling leading to impaired glucose uptake and metabolic dysregulation. The protein's dysfunction is linked to obesity forming a connection through its interactions with other proteins like Akt and mTOR. Aberrant IRS1 activity may contribute to altered cell metabolism and growth promoting conditions like diabetes which highlights the importance of IRS1 in maintaining metabolic health.

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Product protocols

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Target data

Signaling adapter protein that participates in the signal transduction from two prominent receptor tyrosine kinases, insulin receptor/INSR and insulin-like growth factor I receptor/IGF1R (PubMed : 7541045, PubMed : 33991522, PubMed : 38625937). Plays therefore an important role in development, growth, glucose homeostasis as well as lipid metabolism (PubMed : 19639489). Upon phosphorylation by the insulin receptor, functions as a signaling scaffold that propagates insulin action through binding to SH2 domain-containing proteins including the p85 regulatory subunit of PI3K, NCK1, NCK2, GRB2 or SHP2 (PubMed : 11171109, PubMed : 8265614). Recruitment of GRB2 leads to the activation of the guanine nucleotide exchange factor SOS1 which in turn triggers the Ras/Raf/MEK/MAPK signaling cascade (By similarity). Activation of the PI3K/AKT pathway is responsible for most of insulin metabolic effects in the cell, and the Ras/Raf/MEK/MAPK is involved in the regulation of gene expression and in cooperation with the PI3K pathway regulates cell growth and differentiation. Acts a positive regulator of the Wnt/beta-catenin signaling pathway through suppression of DVL2 autophagy-mediated degradation leading to cell proliferation (PubMed : 24616100).

See full target information Insulin receptor substrate 1

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human IRS1 ELISA Kit

Sandwich ELISA - Human IRS1 ELISA Kit (AB289646)

Sandwich ELISA - Human IRS1 ELISA Kit (AB289646)

Key facts

Detection method

Sample types

Reacts with

Assay type

Results type

Sensitivity

Range

Assay time

Assay Platform

Complementary Products

Primary Antibodies

Proteins & Peptides

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise