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AB267651

Human Jagged1 ELISA Kit

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Human Jagged1 ELISA Kit is a Sandwich ELISA for the measurement of Human Jagged1 in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

CD339, JAGL1, JAG1, Protein jagged-1, Jagged1, hJ1

1 Images
Sandwich ELISA - Human Jagged1 ELISA Kit (AB267651)
  • sELISA

Unknown

Sandwich ELISA - Human Jagged1 ELISA Kit (AB267651)

This standard curve is for demonstration only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 1.6 ng/mL

Range

1.638 - 400 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Complementary Products

Primary Antibodies

AB109536

Anti-Jagged1 antibody [EPR4290]

RabMAb|Recombinant|KO Validated

Western blot - Anti-Jagged1 antibody [EPR4290] (AB109536)

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Primary Antibodies

AB307701

Anti-Notch2 antibody [EPR26111-69] - BSA and Azide free

RabMAb|Recombinant

Western blot - Anti-Notch2 antibody [EPR26111-69] - BSA and Azide free (AB307701)

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Proteins & Peptides

AB109346

Recombinant mouse Jagged1 protein (Fc Chimera Active)

Bioactive

Inhibition Assay - Recombinant mouse Jagged1 protein (Fc Chimera Active) (AB109346)

  • 0
  • 0
Primary Antibodies

AB154077

Anti-HEY1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-HEY1 antibody (AB154077)

  • 4
  • 2
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Jagged1 ELISA Kit is designed for the quantitative determination of Jagged1 in cell culture supernatants, plasma and serum samples.

∆Note: Human Jagged1 concentration is low in normal serum/plasma, and may not be detectable in this assay.

This assay employs an antibody specific for human Jagged1 coated on a 96-well plate. Standards and samples are pipetted into the wells and Jagged1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human Jagged1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Jagged1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

This ELISA kit detects human Jagged1. Other species not determined.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
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Recovery

[ { "sample": "Serum", "range": "69 - 90 %", "average": "= 77.24" }, { "sample": "Plasma", "range": "67 - 82 %", "average": "= 70.1" }, { "sample": "Cell culture media", "range": "75 - 127 %", "average": "= 99.66" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab267651-1x96Tests", "assetComponentDetails": [ { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB267651-CMP01", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB267651-CMP05", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB267651-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Human Jagged1 coated Microplate (12 x 8 wells)", "number":"AB267651-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Jagged1 Standard (Lyophilized)", "number":"AB267651-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Human Jagged1 Detection Antibody", "number":"AB267651-CMP08", "productcode":"" }, { "size":"1 x 200 µL", "name":"250X HRP-Streptavidin Concentrate", "number":"AB267651-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Substrate Solution", "number":"AB267651-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Jagged1 also known as JAG1 or Jagged 1 protein is a transmembrane protein involved in the Notch signaling pathway. It has a mass of approximately 150 kDa and is expressed in various tissues including the cardiovascular system liver and mammary glands. Not only does it play a role in cell-cell communication but it also engages in controlling cell differentiation proliferation and apoptosis. Additionally researchers studying its molecular nature frequently employ Jagged1 ELISA kits to measure its expression and activity levels.
Biological function summary

The Jagged1 protein acts as a ligand binding to Notch receptors forming a large complex with other critical proteins within the pathway. This interaction initiates the signal transduction necessary for various cellular processes reinforcing the influence Jagged1 has on development and tissue homeostasis. Its role as a ligand amplifies its importance and scientists often use Jagged1 ELISA and Jag1 ELISA assays to explore these biological interactions further.

Pathways

Research shows that Jagged1 is an integral component of the Notch signaling and cardiovascular development pathways. It binds to Notch1 and Notch2 receptors facilitating important steps in both pathways which are related to embryonic development and adult tissue maintenance. This Notch-Jagged1 interaction also connects to Delta-like ligands creating an intricate network of proteins and pathways critical for a wide spectrum of cellular functions.

Jagged1 exhibits significant connections to Alagille syndrome and some cancers. Mutations in Jagged1 or disrupted interactions with Notch receptors often lead to the developmental defects characteristic of Alagille syndrome where both Jagged1 and Notch2 play significant roles. Additionally aberrant activation of the Notch signaling pathway involving Jagged1 has links to tumorigenesis in certain cancers opining avenues for potential therapeutic interventions against these diseases.
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Product protocols

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Target data

Ligand for multiple Notch receptors and involved in the mediation of Notch signaling (PubMed : 18660822, PubMed : 20437614). May be involved in cell-fate decisions during hematopoiesis (PubMed : 9462510). Seems to be involved in early and late stages of mammalian cardiovascular development. Inhibits myoblast differentiation (By similarity). Enhances fibroblast growth factor-induced angiogenesis (in vitro).
See full target information JAG1
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websiteProtocolBooklet
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Product promise

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