Human JAM-C ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human JAM-C in Human in Serum, Plasma, Cell culture supernatant samples.
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Key facts
- Detection method
- Colorimetric
- Sample types
- Serum, Plasma, Cell culture supernatant
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 0.12 - 30 ng/mL
- Sensitivity
- = 0.12 ng/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Junctional adhesion protein that mediates heterotypic cell-cell interactions with its cognate receptor JAM2 to regulate different cellular processes (PubMed:11590146, PubMed:11823489). Plays a role in homing and mobilization of hematopoietic stem and progenitor cells within the bone marrow. At the surface of bone marrow stromal cells, it contributes to the retention of the hematopoietic stem and progenitor cells expressing JAM3 (PubMed:11590146, PubMed:24357068). Plays a central role in leukocytes extravasation by facilitating transmigration through the endothelium (By similarity). Plays a role in spermatogenesis where JAM2 and JAM3, which are respectively expressed by Sertoli and germ cells, mediate an interaction between both cell types and play an essential role in the anchorage of germ cells onto Sertoli cells and the assembly of cell polarity complexes during spermatid differentiation (By similarity). Also functions as a counter-receptor for ITGAM, mediating leukocyte-platelet interactions and is involved in the regulation of transepithelial migration of polymorphonuclear neutrophils (PMN) (PubMed:12208882, PubMed:15194813). Plays a role in angiogenesis (PubMed:23255084). Plays a role in the regulation of cell migration (Probable). During myogenesis, it is involved in myocyte fusion (By similarity). Soluble form of JAM-C. Promotes chemotaxis of vascular endothelial cells and stimulates angiogenesis.
Alternative names
UNQ859/PRO1868, JAM3, Junctional adhesion molecule C, JAM-C, JAM-2, Junctional adhesion molecule 3, JAM-3, JAMC
What's included?
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Human JAM-C ELISA Kit is a Sandwich (quantitative) ELISA kit for the measurement of Human JAM-C in Human in Serum, Plasma, Cell culture supernatant samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Serum, Plasma, Cell culture supernatant
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 0.12 - 30 ng/mL
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 0.12 ng/mL
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 108.5 | Range 101 - 115 % |
Sample type Plasma | Average % = 100.1 | Range 91 - 109 % |
Sample type Cell culture media | Average % = 103.1 | Range 98 - 104 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- -20°C
Notes
Human JAM-C ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human JAM-C in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human JAM-C coated on a 96-well plate.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Junctional adhesion molecule-C (JAM-C) is a cell adhesion molecule belonging to the immunoglobulin superfamily also known as JAM3. It has a mass around 37 kDa. JAM-C is present on endothelial cells various immune cells and some epithelial cells. This protein functions mechanically in maintaining tight junctions that control paracellular permeability and are important in cell-to-cell interactions.
- Biological function summary
Junctional adhesion molecule-C plays significant roles in leukocyte migration angiogenesis and the maintenance of vascular endothelial barrier function. JAM-C is part of a larger junctional adhesion molecule complex which includes JAM-A and JAM-B that collectively influence these processes. Its expression in tissues such as the heart and brain suggests specialized roles in physiological functions including cardiovascular regulation and CNS barriers.
- Pathways
Junctional adhesion molecule-C is an integral component of the leukocyte transmigration and angiogenesis pathways. These pathways collaborate to allow immune cells to pass through vessel walls a fundamental process in immune surveillance and response. Additionally JAM-C interacts with integrins and other adhesion molecules facilitating cellular communication necessary for tissue repair and growth.
- Associated diseases and disorders
Dysregulation of junctional adhesion molecule-C expression associates with inflammatory diseases and cancer. Altered JAM-C levels have implications in autoimmune disorders where abnormal leukocyte extravasation occurs. Its connection to cancer involves interactions with proteins like VE-cadherin contributing to increased tumor angiogenesis and metastasis making it a potential target for therapeutic intervention in these conditions.
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1 product image
Sandwich ELISA - Human JAM-C ELISA Kit (ab313977)
Example of standard curve using ab313977. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.
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Product protocols
- Visit the general protocols
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