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AB213800

Human Junctional Adhesion Molecule 1/JAM-A ELISA Kit

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(2 Publications)

Human Junctional Adhesion Molecule 1/JAM-A ELISA Kit is a Sandwich ELISA for the measurement of Human Junctional Adhesion Molecule 1/JAM-A in Human in Cell Culture Media, Biofluids samples.

View Alternative Names

CD321, JAM1, JCAM, UNQ264/PRO301, F11R, Junctional adhesion molecule A, JAM-A, Junctional adhesion molecule 1, Platelet F11 receptor, Platelet adhesion molecule 1, JAM-1, PAM-1

1 Images
Sandwich ELISA - Human Junctional Adhesion Molecule 1/JAM-A ELISA Kit (AB213800)
  • sELISA

Supplier Data

Sandwich ELISA - Human Junctional Adhesion Molecule 1/JAM-A ELISA Kit (AB213800)

Human JAM-A ELISA Kit (F11R) (ab213800) Standard Curve.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

< 10 pg/mL

Range

46.9 - 3000 pg/mL

Assay time

3h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Complementary Products

Primary Antibodies

AB270446

Anti-Junctional Adhesion Molecule 1/JAM-A antibody [EPR23246-275]

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Immunocytochemistry/ Immunofluorescence - Anti-Junctional Adhesion Molecule 1/JAM-A antibody [EPR23246-275] (AB270446)

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Secondary Antibodies

AB150155

Donkey Anti-Rat IgG H&L (Alexa Fluor® 647) preadsorbed

Immunocytochemistry/ Immunofluorescence - Donkey Anti-Rat IgG H&L (Alexa Fluor® 647) preadsorbed (AB150155)

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Assay Kits

AB252355

Mouse ICAM 1 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Mouse ICAM 1 ELISA Kit (AB252355)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

The Human Junctional Adhesion Molecule 1/JAM-A Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213800) is designed for the quantitative detection of Human JAM-A in cell culture supernatants and serum.

The ELISA kit is based on standard sandwich enzyme-linked immunosorbent assay technology. A monoclonal antibody from mouse specific for JAM-A has been pre-coated onto 96-well plates. Standards (Expression system for standard: E.coli; Immunogen sequence: S28-V238) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for JAM-A is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human JAM-A amount of sample captured in plate.

Junctional adhesion molecule A/JAM-A is a protein that in humans is encoded by the F11R gene. It is mapped to 1q23.3. This gene is an immunoglobulin-like molecule that co-localizes with tight junctions in endothelium and epithelium and is also found on blood leukocytes and platelets. JAM-A plays an important role in the regulation of tight junction assembly in epithelia. In addition, it can act as a receptor for reovirus, a ligand for the integrin LFA1, involved in leukocyte transmigration and a platelet receptor. JAM-A has a non-redundant role in controlling DC motility, trafficking to lymph nodes, and activation of specific immunity.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Sample 1", "replicates": 24, "mean": "73 pg/mL", "standardDeviation": "5.32", "coefficientOfVariability": "7.3" }, { "reproducibilityType": "Inter", "sample": "Sample 2", "replicates": 24, "mean": "310 pg/mL", "standardDeviation": "16.43", "coefficientOfVariability": "5.3" }, { "reproducibilityType": "Inter", "sample": "Sample 3", "replicates": 24, "mean": "1209 pg/mL", "standardDeviation": "93.09", "coefficientOfVariability": "7.7" }, { "reproducibilityType": "Intra", "sample": "Sample 1", "replicates": 16, "mean": "81 pg/mL", "standardDeviation": "5.83", "coefficientOfVariability": "7.2" }, { "reproducibilityType": "Intra", "sample": "Sample 2", "replicates": 16, "mean": "325 pg/mL", "standardDeviation": "16.9", "coefficientOfVariability": "5.2" }, { "reproducibilityType": "Intra", "sample": "Sample 3", "replicates": 16, "mean": "1280 pg/mL", "standardDeviation": "67.84", "coefficientOfVariability": "5.3" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Junctional Adhesion Molecule 1 also known as JAM-A or JAM-A protein is a member of the immunoglobulin superfamily. It has a mass of roughly 32 kDa. This molecule functions in tight junctions of epithelial and endothelial cells playing an important role in regulating paracellular permeability. JAM-A is expressed in tissues where tight junctions are present including the skin gastrointestinal tract and endothelia throughout the body.
Biological function summary

JAM-A functions as a cell adhesion molecule influencing processes like leukocyte transmigration and epithelial barrier formation. It does not act alone but rather forms complexes with other proteins at cell junctions. JAM-A interacts with other junctional proteins like occludin and claudins contributing to the structure and function of tight junctions.

Pathways

JAM-A participates in cellular processes involving inflammation and cell migration. It is an integral part of the signal transduction pathways that regulate leukocyte migration an important aspect of immune response. JAM-A is also associated with G-protein coupled receptors in these pathways highlighting its role in regulating intracellular signaling that affects cell adhesion and migration.

JAM-A has connections with cancer progression and cardiovascular diseases. Its overexpression has been linked to several cancers including breast cancer due to its role in enhancing cell migration and invasion. In cardiovascular diseases JAM-A interacts with platelet-endothelial cell adhesion molecule-1 (PECAM-1) influencing the inflammatory response within vascular tissues. Understanding these interactions can aid in developing therapeutic strategies targeting JAM-A in related diseases.
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Product protocols

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Target data

Seems to play a role in epithelial tight junction formation. Appears early in primordial forms of cell junctions and recruits PARD3 (PubMed : 11489913). The association of the PARD6-PARD3 complex may prevent the interaction of PARD3 with JAM1, thereby preventing tight junction assembly (By similarity). Plays a role in regulating monocyte transmigration involved in integrity of epithelial barrier (By similarity). Ligand for integrin alpha-L/beta-2 involved in memory T-cell and neutrophil transmigration (PubMed : 11812992). Involved in platelet activation (PubMed : 10753840).. (Microbial infection) Acts as a receptor for Mammalian reovirus sigma-1.. (Microbial infection) Acts as a receptor for Human Rotavirus strain Wa.
See full target information F11R
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Yonsei medical journal 64:375-383 PubMed37226564

2023

Impact of the Junction Adhesion Molecule-A on Asthma.

Applications

Unspecified application

Species

Unspecified reactive species

Min-Hyeok An,Pureun-Haneul Lee,Seon-Muk Choi,DaYeon Hwang,Jung-Hyun Kim,Meung Chul Park,Shinhee Park,Ae-Rin Baek,An-Soo Jang

Frontiers in neurology 12:721118 PubMed34531816

2021

MMP-9 as a Biomarker for Predicting Hemorrhagic Strokes in Moyamoya Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Junlin Lu,Jingyi Wang,Zhidong Lin,Guangchao Shi,Rong Wang,Yahui Zhao,Yuanli Zhao,Jizong Zhao
View all publications
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Product promise

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