Human Kallikrein 10 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Kallikrein 10 with a sensitivity of 5.29 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
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Key facts
- Detection method
- Colorimetric
- Sample types
- Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 10.94 - 700 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 5.29 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Has a tumor-suppressor role for NES1 in breast and prostate cancer.
Alternative names
NES1, PRSSL1, KLK10, Kallikrein-10, Normal epithelial cell-specific 1, Protease serine-like 1
What's included?
Recommended products
Human Kallikrein 10 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Kallikrein 10 with a sensitivity of 5.29 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
- Detection method
- Colorimetric
- Sample types
- Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 10.94 - 700 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 5.29 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 8 | mean - | SD - | C.V. 4.4 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 3 | mean - | SD - | C.V. 4.6 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 113 | Range 110 - 115 % |
Sample type Serum | Average % = 101 | Range 91 - 110 % |
Sample type EDTA Plasma | Average % = 103 | Range 86 - 112 % |
Sample type Heparin Plasma | Average % = 101 | Range 92 - 107 % |
Sample type Citrate plasma | Average % = 107 | Range 102 - 115 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human Kallikrein 10 ELISA kit (ab279406) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human Kallikrein 10 protein in human serum, plasma, and cell culture supernatant samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Kallikrein 10 with 5.29 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Kallikrein 10 is a member of a family of serine proteases that are involved in a variety of cellular functions. Kallikrein 10 has been linked to carcinogenesis as having a tumor suppressor role in breast and prostate cancer. Kallikrein 10 is upregulated in gastrointestinal cancers but downregulated in breast cancer, prostate cancer, NSC lung cancer and renal call carcinoma.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Kallikrein 10 also known as KLK10 or serine protease 20 is a protein known for its enzymatic activity. It has a mass of approximately 30 kDa and expresses highly in tissues like the pancreas kidney and hormonal dependent tissues such as the prostate and breast. KLK10 involves in the degradation of extracellular matrix components functioning as a serine protease. Its expression in hormone-responsive tissues indicates a potential role in processes like tissue remodeling and regulation.
- Biological function summary
Kallikrein 10 is part of a larger family of serine proteases involved in various physiological processes. These proteases participate in tissue homeostasis and in some instances immune response. KLK10 expression links to cell proliferation and survival potentially influencing cancer progression. The network of serine proteases forms a proteolytic cascade where multiple proteases sequentially activate amplifying the biological responses.
- Pathways
Kallikrein 10 plays a critical role in the proteolytic cascade responsible for regulating homeostatic processes. It participates notably in pathways such as the kallikrein-kinin system and the regulation of tumor microenvironment. KLK10 interacts with other kallikreins like KLK5 and KLK6 mediating their activation and functioning in tissue remodeling and repair processes.
- Associated diseases and disorders
Kallikrein 10 has a significant association with cancers and inflammatory conditions. Altered KLK10 expression shows correlation with prostate cancer and breast cancer where it might serve as a biomarker for diagnosis or prognosis. KLK10's involvement with proteins like KLK3 (PSA) in prostate cancer reflects its potential utility in therapeutic strategies especially concerning hormone-dependent tumors.
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3 product images
Sandwich ELISA - Human Kallikrein 10 ELISA Kit (ab279406)
Example of human Kallikrein 10 standard curve in Sample Diluent NS.
The Kallikrein 10 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human Kallikrein 10 ELISA Kit (ab279406)
Interpolated concentrations of native Kallikrein 10 in human serum and plasma samples.
The concentrations of Kallikrein 10 were measured in duplicates, interpolated from the Kallikrein 10 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 100%, plasma (citrate) 50%, plasma (heparin) 100%, and plasma (EDTA) 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Kallikrein 10 concentration was determined to be 263.09 pg/mL in serum, 526.64 pg/mL in plasma (citrate), 380.88 pg/mL in plasma (heparin), and 195.75 pg/mL in plasma (EDTA).
Sandwich ELISA - Human Kallikrein 10 ELISA Kit (ab279406)
Serum from ten individual healthy human female donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Kallikrein 10 concentration was determined to be 1,062.93 pg/mL with a range of 728.05 – 1,332.52 pg/mL.
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