AB279406

Human Kallikrein 10 ELISA Kit

Recombinant
SimpleStep
What is this?

Be the first to review this product! Submit a review

(0 Publication)

Human Kallikrein 10 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Kallikrein 10 with a sensitivity of 5.29 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

NES1, PRSSL1, KLK10, Kallikrein-10, Normal epithelial cell-specific 1, Protease serine-like 1

3 Images

Sandwich ELISA - Human Kallikrein 10 ELISA Kit (AB279406)

sELISA

Supplier Data

Sandwich ELISA - Human Kallikrein 10 ELISA Kit (AB279406)

Example of human Kallikrein 10 standard curve in Sample Diluent NS.

The Kallikrein 10 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human Kallikrein 10 ELISA Kit (AB279406)

Serum from ten individual healthy human female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Kallikrein 10 concentration was determined to be 1,062.93 pg/mL with a range of 728.05 – 1,332.52 pg/mL.

sELISA

Supplier Data

Sandwich ELISA - Human Kallikrein 10 ELISA Kit (AB279406)

Interpolated concentrations of native Kallikrein 10 in human serum and plasma samples.

The concentrations of Kallikrein 10 were measured in duplicates, interpolated from the Kallikrein 10 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 100%, plasma (citrate) 50%, plasma (heparin) 100%, and plasma (EDTA) 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Kallikrein 10 concentration was determined to be 263.09 pg/mL in serum, 526.64 pg/mL in plasma (citrate), 380.88 pg/mL in plasma (heparin), and 195.75 pg/mL in plasma (EDTA).

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 5.29 pg/mL

Range

10.94 - 700 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

style

left-column

Product details

Human Kallikrein 10 ELISA kit (ab279406) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human Kallikrein 10 protein in human serum, plasma, and cell culture supernatant samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Kallikrein 10 with 5.29 pg/mL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Kallikrein 10 is a member of a family of serine proteases that are involved in a variety of cellular functions. Kallikrein 10 has been linked to carcinogenesis as having a tumor suppressor role in breast and prostate cancer. Kallikrein 10 is upregulated in gastrointestinal cancers but downregulated in breast cancer, prostate cancer, NSC lung cancer and renal call carcinoma.

style

left-column

Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.6" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.4" } ]

style

left-column

Recovery

[ { "sample": "Cell culture supernatant", "range": "110 - 115 %", "average": "= 113" }, { "sample": "Serum", "range": "91 - 110 %", "average": "= 101" }, { "sample": "EDTA Plasma", "range": "86 - 112 %", "average": "= 103" }, { "sample": "Heparin Plasma", "range": "92 - 107 %", "average": "= 101" }, { "sample": "Citrate plasma", "range": "102 - 115 %", "average": "= 107" } ]

style

left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab279406-1x96Tests", "assetComponentDetails": [ { "size":"1 x 600 µL", "name":"10X Human Kallikrein 10 Capture Antibody", "number":"AB279406-CMP03", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB279406-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB279406-CMP11", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB279406-CMP12", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Kallikrein 10 Lyophilized Recombinant Protein", "number":"AB279406-CMP08", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB279406-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB279406-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB279406-CMP09", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Kallikrein 10 Detector Antibody", "number":"AB279406-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB279406-CMP07", "productcode":"" } ] } } }

style

left-column

Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Kallikrein 10 also known as KLK10 or serine protease 20 is a protein known for its enzymatic activity. It has a mass of approximately 30 kDa and expresses highly in tissues like the pancreas kidney and hormonal dependent tissues such as the prostate and breast. KLK10 involves in the degradation of extracellular matrix components functioning as a serine protease. Its expression in hormone-responsive tissues indicates a potential role in processes like tissue remodeling and regulation.

Biological function summary

Kallikrein 10 is part of a larger family of serine proteases involved in various physiological processes. These proteases participate in tissue homeostasis and in some instances immune response. KLK10 expression links to cell proliferation and survival potentially influencing cancer progression. The network of serine proteases forms a proteolytic cascade where multiple proteases sequentially activate amplifying the biological responses.

Pathways

Kallikrein 10 plays a critical role in the proteolytic cascade responsible for regulating homeostatic processes. It participates notably in pathways such as the kallikrein-kinin system and the regulation of tumor microenvironment. KLK10 interacts with other kallikreins like KLK5 and KLK6 mediating their activation and functioning in tissue remodeling and repair processes.

Kallikrein 10 has a significant association with cancers and inflammatory conditions. Altered KLK10 expression shows correlation with prostate cancer and breast cancer where it might serve as a biomarker for diagnosis or prognosis. KLK10's involvement with proteins like KLK3 (PSA) in prostate cancer reflects its potential utility in therapeutic strategies especially concerning hormone-dependent tumors.

style

left-column

Product protocols

style

left-column

Target data

Has a tumor-suppressor role for NES1 in breast and prostate cancer.

See full target information KLK10

style

left-column

style

left-column

websiteProtocolBooklet

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human Kallikrein 10 ELISA Kit

Sandwich ELISA - Human Kallikrein 10 ELISA Kit (AB279406)

Sandwich ELISA - Human Kallikrein 10 ELISA Kit (AB279406)

Sandwich ELISA - Human Kallikrein 10 ELISA Kit (AB279406)

Key facts

Detection method

Sample types

Reacts with

Assay type

Results type

Sensitivity

Range

Assay time

Assay Platform

Complementary Products

Primary Antibodies

Assay Kits

Assay Kits

Assay Kits

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise