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AB213802

Human Kallikrein 12 ELISA Kit

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Human Kallikrein 12 ELISA Kit is a Sandwich ELISA for the measurement of Human Kallikrein 12 in Human in Cell/Tissue Extracts, Cell Culture Media, Biofluids samples.
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Sandwich ELISA - Human Kallikrein 12 ELISA Kit (AB213802)
  • sELISA

Supplier Data

Sandwich ELISA - Human Kallikrein 12 ELISA Kit (AB213802)

Human KLK12 ELISA Kit (ab213802) Standard Curve.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate, Cell culture supernatant, EDTA Plasma, Heparin Plasma, Serum, Tissue Homogenate

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

< 10 pg/mL

Range

156 - 10000 pg/mL

Assay time

3h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

The Human Kallikrein 12 (KLK12) Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213802) is designed for the quantitative measurement of Human KLK12 in cell culture supernatants, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).

The ELISA kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for KLK12 has been pre-coated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for KLK12 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human KLK12 amount of sample captured in plate.

Kallikrein-12 is a protein that in humans is encoded by the KLK12 gene. It belongs to the kallikrein subgroup of serine proteases, which have diverse physiologic functions in many tissues. KLK12 is expressed primarily in salivary gland, stomach, uterus, trachea, prostate, thymus, lung, colon, brain, breast, and thyroid gland and at lower levels in testis, pancreas, small intestine, and spinal cord. The expression of KLK12 is downregulated at the mRNA level in breast cancer tissues and is upregulated by steroid hormones in breast and prostate cancer cell lines.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Sample 1", "replicates": 24, "mean": "1523 pg/mL", "standardDeviation": "89.85", "coefficientOfVariability": "5.9" }, { "reproducibilityType": "Inter", "sample": "Sample 2", "replicates": 24, "mean": "4129 pg/mL", "standardDeviation": "251.86", "coefficientOfVariability": "6.1" }, { "reproducibilityType": "Inter", "sample": "Sample 3", "replicates": 24, "mean": "6526 pg/mL", "standardDeviation": "443.76", "coefficientOfVariability": "6.8" }, { "reproducibilityType": "Intra", "sample": "Sample 1", "replicates": 16, "mean": "1409 pg/mL", "standardDeviation": "73.26", "coefficientOfVariability": "5.2" }, { "reproducibilityType": "Intra", "sample": "Sample 2", "replicates": 16, "mean": "3882 pg/mL", "standardDeviation": "213.51", "coefficientOfVariability": "5.5" }, { "reproducibilityType": "Intra", "sample": "Sample 3", "replicates": 16, "mean": "6756 pg/mL", "standardDeviation": "405.36", "coefficientOfVariability": "6" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Kallikrein 12 also known as KLK12 belongs to the kallikrein family of serine proteases. This family plays significant roles in various physiological processes. Kallikrein 12 has a mass of approximately 27 kDa. It is expressed in a range of tissues but shows higher expression in the skin respiratory tract and brain tissues. This distribution suggests that Kallikrein 12 may have diverse functional roles depending on its location in the body.
Biological function summary

The KLK12 protein influences complex processes involving extracellular matrix remodeling and possibly tissue homeostasis. It functions as part of a larger group of kallikreins which collectively impact the proteolytic cascade mechanisms. The activity of KLK12 is regulated by specific inhibitors which maintain balance in protease activity within the tissues ensuring normal cell and tissue functions.

Pathways

KLK12 plays a role in protease cascades involved in tissue repair and inflammation. The kallikrein-kinin system is one of the critical pathways where KLK12 contributes. This system affects blood pressure regulation and inflammatory responses. KLK12 associates with proteins such as kininogens and other kallikreins which together facilitate its involvement in these biological processes impacting both vascular biology and immune responses.

KLK12 has associations with certain skin disorders and respiratory diseases. Alterations in KLK12 expression are observed in conditions like asthma where the protein's role in inflammation may influence disease progression. KLK12 associates with other kallikrein family members in these disorders highlighting the potential for multiple kallikreins to impact pathophysiological conditions. Improper regulation of KLK12 activity can contribute to these disease states making it a potential target for therapeutic interventions.
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Product protocols

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Target data

See full target information KLK12

Alternative Names

KLKL5, UNQ669/PRO1303, KLK12, Kallikrein-12, Kallikrein-like protein 5, KLK-L5
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Product promise

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