JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB213803

Human Kallikrein 13 ELISA Kit

Be the first to review this product! Submit a review

|

(0 Publication)

Human Kallikrein 13 ELISA Kit is a Sandwich ELISA for the measurement of Human Kallikrein 13 in Human in Cell/Tissue Extracts, Cell Culture Media, Biofluids samples.

View Alternative Names

KLKL4, KLK13, Kallikrein-13, Kallikrein-like protein 4, KLK-L4

1 Images
Sandwich ELISA - Human Kallikrein 13 ELISA Kit (AB213803)
  • sELISA

Supplier Data

Sandwich ELISA - Human Kallikrein 13 ELISA Kit (AB213803)

Human KLK13 ELISA Kit (ab213803) Standard Curve.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate, Cell culture supernatant, EDTA Plasma, Heparin Plasma, Serum, Tissue Homogenate

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

< 10 pg/mL

Range

156 - 10000 pg/mL

Assay time

3h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style
left-column

Complementary Products

Assay Kits

AB213820

Human Relaxin 1 ELISA Kit

Sandwich ELISA - Human Relaxin 1 ELISA Kit (AB213820)

  • 0
  • 0
Assay Kits

AB309433

Human 15-PGDH ELISA Kit

Sandwich ELISA - Human 15-PGDH ELISA Kit (AB309433)

  • 0
  • 0
Primary Antibodies

AB126597

Anti-PON1 antibody [EPR2893]

RabMAb|Recombinant

Immunoprecipitation - Anti-PON1 antibody [EPR2893] (AB126597)

  • 0
  • 1
Primary Antibodies

AB273106

Anti-SMAD6 antibody [EPR24687-21]

20ul selling size|RabMAb|Recombinant

Western blot - Anti-SMAD6 antibody [EPR24687-21] (AB273106)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

The Human Kallikrein 13 (KLK13) Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213803) is designed for the quantitative measurement of Human KLK13 in cell culture supernatants, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).

The ELISA kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for KLK13 has been pre-coated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for KLK13 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human KLK13 amount of sample captured in plate.

Kallikrein-13 is a protein that in humans is encoded by the KLK13 gene. It belongs to the kallikrein subgroup of serine proteases, which have diverse physiologic functions in many tissues. By genomic sequence analysis, KLK13 gene is mapped in a 300-kb region on chromosome 19q13.3-q13.4. It has been shown that recombinant hK13 produced in yeast can cleave synthetic peptides after the arginine residue and some extracellular matrix components. However, its exact physiological substrates and functions remain obscure. Despite the lack of knowledge on the physiological function of hK13, several studies have demonstrated that hK13 is implicated with cancer of the breast and ovary and it can serve as a favorable prognostic biomarker for these malignancies.

style
left-column

Precision

[ { "reproducibilityType": "Inter", "sample": "Sample 1", "replicates": 24, "mean": "1369 pg/mL", "standardDeviation": "71.18", "coefficientOfVariability": "5.2" }, { "reproducibilityType": "Inter", "sample": "Sample 2", "replicates": 24, "mean": "4425 pg/mL", "standardDeviation": "269.92", "coefficientOfVariability": "6.1" }, { "reproducibilityType": "Inter", "sample": "Sample 3", "replicates": 24, "mean": "6594 pg/mL", "standardDeviation": "422.01", "coefficientOfVariability": "6.4" }, { "reproducibilityType": "Intra", "sample": "Sample 1", "replicates": 16, "mean": "1661 pg/mL", "standardDeviation": "83.05", "coefficientOfVariability": "5" }, { "reproducibilityType": "Intra", "sample": "Sample 2", "replicates": 16, "mean": "3572 pg/mL", "standardDeviation": "196.46", "coefficientOfVariability": "5.5" }, { "reproducibilityType": "Intra", "sample": "Sample 3", "replicates": 16, "mean": "6985 pg/mL", "standardDeviation": "405.13", "coefficientOfVariability": "5.8" } ]
style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab213803-1x96Tests", "assetComponentDetails": [ { "size":"1 x 100 µL", "name":"Avidin-Biotin-Peroxidase Complex (ABC)", "number":"AB213803-CMP10", "productcode":"" }, { "size":"1 x 100 µL", "name":"Biotinylated KLK13 Antibody", "number":"AB213803-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Human KLK13 coated Microplate (12 x 8 wells)", "number":"AB213803-CMP04", "productcode":"" }, { "size":"1 x 10 mL", "name":"TMB Color Developing Agent", "number":"AB213803-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"Antibody Diluent Buffer", "number":"AB213803-CMP08", "productcode":"" }, { "size":"1 x 30 mL", "name":"Sample Diluent Buffer", "number":"AB213803-CMP02", "productcode":"" }, { "size":"4 x 1 Unit", "name":"Adhesive Plate Seal", "number":"AB213803-CMP07", "productcode":"" }, { "size":"1 x 10 mL", "name":"TMB Stop Solution", "number":"AB213803-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human KLK13 Standard (Lyophilized)", "number":"AB213803-CMP01", "productcode":"" }, { "size":"1 x 20 mL", "name":"Wash Buffer (25X)", "number":"AB213803-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"ABC Diluent Buffer", "number":"AB213803-CMP03", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Kallikrein-related peptidase 13 often called KLK13 is a member of the kallikrein family of secreted serine proteases. The protein has a mass of about 30 kDa. KLK13 is known for its enzymatic activities that can cleave peptide bonds in proteins or peptides. It is mainly expressed in tissues such as the skin prostate and salivary glands. Through enzymatic cleavage KLK13 regulates various physiological processes by modulating the activity or availability of target substrates.
Biological function summary

KLK13 functions beyond simple proteolysis and plays a significant role in tissue remodeling and homeostasis. It participates in the regulation of other proteins via signal pathways or proteolytic cascades. KLK13 is not known to be a part of any specific protein complex but indirectly affects several cellular processes through its action on substrate proteins. It can influence processes like wound healing and inflammation.

Pathways

KLK13 contributes to proteolytic pathways relevant to skin desquamation and cancer progression. It interacts with components of the kallikrein-kinin system and is involved in the regulation of kinins which are mediator proteins. Kinins play roles in inflammation and vascular functions. KLK13 also shows interaction with other kallikrein family members suggesting cooperative or redundant roles in physiological and pathological processes.

KLK13 has associations with conditions such as prostate cancer and skin disorders. In prostate cancer dysregulation of KLK13 may facilitate tumor progression or metastasis. This involvement often links KLK13 to other kallikrein-related peptidases like KLK2 which also participate in cancer biology. In skin disorders aberrant KLK13 activity can disrupt normal skin barrier functions potentially leading to conditions like psoriasis or atopic dermatitis.
style
left-column

Product protocols

style
left-column

Target data

See full target information KLK13
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com