Human Kallikrein 14 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Kallikrein 14 with a sensitivity of 9.06 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
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Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture media, Citrate plasma, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 14.06 - 900 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 9.06 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Serine-type endopeptidase with a dual trypsin-like and chymotrypsin-like substrate specificity. May activate/inactivate the proteinase-activated receptors F2R, F2RL1 and F2RL3 and other kallikreins including KLK1, KLK3, KLK5 and KLK11. May function in seminal clot liquefaction through direct cleavage of the semenogelin SEMG1 and SEMG2 and activation of KLK3. May function through desmoglein DSG1 cleavage in epidermal desquamation a process by which the most superficial corneocytes are shed from the skin surface. May be involved in several aspects of tumor progression including growth, invasion and angiogenesis.
Alternative names
KLKL6, KLK14, Kallikrein-14, hK14, Kallikrein-like protein 6, KLK-L6
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Human Kallikrein 14 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Kallikrein 14 with a sensitivity of 9.06 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell culture media, Citrate plasma, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 14.06 - 900 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 9.06 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 8 | mean - | SD - | C.V. 5.8 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 3 | mean - | SD - | C.V. 14.9 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 111 | Range 102 - 124 % |
Sample type Cell culture media | Average % = 115 | Range 111 - 118 % |
Sample type Citrate plasma | Average % = 109 | Range 105 - 112 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human Kallikrein 14 ELISA Kit (ab283870) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Kallikrein 14 protein in serum, plasma and cell culture supernatant samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Kallikrein 14 with 9.06 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Kallikrein 14 is a serine protease and member of the kallikrein family of proteases. It has been implicated in carcinogenesis and skin disorders. Kallikrein 14 is activated by KLK5, and its activation leads to a downstream activation of additional kallikreins.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Kallikrein 14 (KLK14) a serine protease is involved in various proteolytic activities. It has a molecular mass of approximately 25 kDa. KLK14 is part of the kallikrein-related peptidase family and is expressed in diverse tissues including skin prostate and brain. This protein is also known by the alternate name hK14. The expression pattern of KLK14 suggests its involvement in local tissue-specific functions.
- Biological function summary
KLK14 influences processes like skin desquamation and seminal plasma liquefaction. It plays a significant role in the regulation of extracellular matrix components and participates in activating protein precursors. Though it functions as an individual protease KLK14 interacts with other kallikreins in a possible cascade-like manner indicating complex interplay between these proteins.
- Pathways
KLK14 is key in processes involving the kallikrein-kinin system and the activation of certain growth factors. It can cleave and activate other proteins such as renin-angiotensin system components influencing blood pressure regulation. KLK14 operates within this intricate network alongside proteins like KLK1 and other kallikreins that have complementary roles in physiological pathways.
- Associated diseases and disorders
KLK14 has been linked to prostate cancer and skin-related conditions such as atopic dermatitis. Overexpression of KLK14 correlates with prostate cancer progression suggesting its involvement in tumorigenesis. It is also associated with other kallikreins like KLK3 (PSA) in cancer influencing disease development and prognosis. In skin disorders KLK14 may affect processes like inflammation and barrier function revealing its therapeutic target potential.
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3 product images
Sandwich ELISA - Human Kallikrein 14 ELISA Kit (ab283870)
Example of human Kallikrein 14 standard curve in Sample Diluent NS.
The Kallikrein 14 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human Kallikrein 14 ELISA Kit (ab283870)
Interpolated concentrations of native Kallikrein 14 in human serum and plasma (citrate) samples.
The concentrations of Kallikrein 14 were measured in duplicates, interpolated from the Kallikrein 14 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 100% and plasma (citrate) 100% The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Kallikrein 14 concentration was determined to be 149.55 pg/mL in serum and 206.62 pg/mL in plasma (citrate).
Sandwich ELISA - Human Kallikrein 14 ELISA Kit (ab283870)
Serum from ten individual healthy human female donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Kallikrein 14 concentration was determined to be 376.65 pg/mL with a range of 138.87 – 708.87 pg/mL.
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