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AB313979

Human Kallikrein 15 ELISA Kit

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Human Kallikrein 15 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human Kallikrein 15 in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

Kallikrein-15, ACO protease, KLK15

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Sandwich ELISA - Human Kallikrein 15 ELISA Kit (AB313979)
  • sELISA

Supplier Data

Sandwich ELISA - Human Kallikrein 15 ELISA Kit (AB313979)

Example of standard curve using ab313979. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Serum, Plasma, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 22 pg/mL

Range

22 - 5000 pg/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Kallikrein 15 ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Kallikrein 15 in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human Kallikrein 15 coated on a 96-well plate.
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Recovery

[ { "sample": "Serum", "range": "83 - 132 %", "average": "= 109.8" }, { "sample": "Plasma", "range": "81 - 105 %", "average": "= 90.66" }, { "sample": "Cell culture media", "range": "120 - 138 %", "average": "= 127.1" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab313979-1x96Tests", "assetComponentDetails": [ { "size":"1 x 200 µL", "name":"100X HRP-Streptavidin", "number":"AB313979-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human Kallikrein 15 Antibody-coated ELISA Plate", "number":"AB313979-CMP08", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB313979-CMP06", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent (Item E2)", "number":"AB313979-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human Kallikrein 15 Detection Antibody", "number":"AB313979-CMP09", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB313979-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB313979-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human Kallikrein 15 Protein Standard", "number":"AB313979-CMP10", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Kallikrein 15 (KLK15) is a serine protease known for its involvement in various physiological processes. This protein has a molecular mass of about 27-28 kDa. KLK15 is part of the kallikrein family and it is often found expressed in tissues such as the prostate uterus and colon. Due to its expression pattern KLK15 is sometimes referred to by alternative names but they are mostly related to tissue-specific variations. The mechanical function involves proteolytic activity where it cleaves peptide bonds in protein substrates.
Biological function summary

KLK15 plays a role in processes like tissue remodeling and can influence cell migration or invasion. As part of the serine protease family it does not just work in isolation but interacts within a network of proteins. There are suggestions of it forming complexes especially with other kallikreins affecting their activity within the local environmental contexts like the extracellular matrix. These actions highlight the biological significance of KLK15 beyond simple catalytic activity.

Pathways

KLK15 participates in the proteolytic cascade pathways. It is closely linked with pathways involved in inflammation and tissue homeostasis. Through these pathways KLK15 interacts with proteins such as kallikrein-related peptidases that share regulatory mechanisms and overlapping substrate specificities. These interactions suggest a role in signaling pathways that regulate cellular responses to environmental changes further linking KLK15 to broader physiological functions.

KLK15 has been associated with prostate cancer and ulcerative colitis. Its expression levels can serve as a biomarker for the diagnosis and progression of prostate cancer highlighting its clinical utility. In prostate cancer KLK15 frequently interacts with proteins like KLK3 also known as prostate-specific antigen (PSA) which is instrumental in managing patient prognosis. Additionally in the context of ulcerative colitis changes in KLK15 expression levels could also provide insight into disease mechanisms or progression although the precise interactions remain an area for further investigation.
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Product protocols

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Target data

Protease whose physiological substrate is not yet known.
See full target information Kallikrein-15
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websiteProtocolBooklet
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Product promise

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