AB287163

Human Kallikrein 6/KLK6 ELISA Kit

Recombinant
SimpleStep
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Human Kallikrein 6/KLK6 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Kallikrein 6/KLK6 with a sensitivity of 15.21 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

PRSS18, PRSS9, KLK6, Kallikrein-6, Neurosin, Protease M, SP59, Serine protease 18, Serine protease 9, Zyme

4 Images

Sandwich ELISA - Human Kallikrein 6/KLK6 ELISA Kit (AB287163)

sELISA

Supplier Data

Sandwich ELISA - Human Kallikrein 6/KLK6 ELISA Kit (AB287163)

Interpolated concentrations of human Kallikrein 6 in serum, plasma (citrate), plasma (heparin), and plasma (EDTA).

Interpolated concentration of native Kallikrein 6 was measured in duplicate at different sample concentrations. Undiluted samples are 50% serum, plasma (citrate), plasma (heparin), and plasma (EDTA). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Supplier Data

Sandwich ELISA - Human Kallikrein 6/KLK6 ELISA Kit (AB287163)

Interpolated concentrations of human Kallikrein 6 in A431 supernatant.

Interpolated concentration of native Kallikrein 6 was measured in duplicate at different sample concentrations. Undiluted samples are 12.50% A431 supernatant. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Supplier Data

Sandwich ELISA - Human Kallikrein 6/KLK6 ELISA Kit (AB287163)

Interpolated concentrations of Kallikrein 6 in normal human serum donors.

Serum of ten individual healthy human female donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +- SD, n=2). The mean Kallikrein 6 concentration was determined to be 1.28 ng/mL with a range of 0.62 – 2.21 ng/mL.

sELISA

Supplier Data

Sandwich ELISA - Human Kallikrein 6/KLK6 ELISA Kit (AB287163)

Example of human Kallikrein 6 standard curve in Sample Diluent NS.

Example of human Kallikrein 6 standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell culture media, Heparin Plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 15.21 pg/mL

Range

23.44 - 1500 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human Kallikrein 6/KLK6 ELISA kit (ab287163) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human Kallikrein 6/KLK6 protein in human serum, plasma and cell culture supernatant samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Kallikrein 6/KLK6 with 15.21 pg/mL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.7" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.3" } ]

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Recovery

[ { "sample": "Serum", "range": "90 - 91 %", "average": "= 91" }, { "sample": "EDTA Plasma", "range": "100 - 104 %", "average": "= 102" }, { "sample": "Cell culture media", "range": "111 - 123 %", "average": "= 116" }, { "sample": "Heparin Plasma", "range": "86 - 89 %", "average": "= 88" }, { "sample": "Citrate plasma", "range": "93 - 99 %", "average": "= 95" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Kallikrein 6 also known as KLK6 is a serine protease with notable enzymatic properties. This protein part of the kallikrein family weighs approximately 25 kDa and exhibits chymotrypsin-like activity. KLK6 is highly expressed in the central nervous system and various body fluids such as cerebrospinal fluid and milk. Due to this distribution KLK6 may play roles in neurological and physiological processes.

Biological function summary

KLK6 participates in the degradation of extracellular matrix components. It functions within complex proteolytic cascades facilitating tissue remodeling and repair. Additionally KLK6 interacts with other kallikreins like kallikrein 3 showing intricate regulation within the kallikrein-related peptidase network. This interplay points toward its importance in modulating extracellular environment and cellular interactions.

Pathways

KLK6 engages in the neurogenic and inflammatory response pathways. It ties into MAPK signaling influencing processes such as cell proliferation and apoptosis. Through these pathways KLK6 associates with proteins like MMPs which modulate extracellular matrix dynamics. Its presence in these pathways positions KLK6 as a significant regulatory protein during cellular homeostasis and stress responses.

KLK6 correlates with neurodegenerative diseases like Alzheimer's and multiple sclerosis. Altered expression and activity levels of KLK6 have implications for disease progression and pathology. In Alzheimer's KLK6 may influence amyloid-beta processing connecting with proteins like APP. In multiple sclerosis KLK6 affects demyelination processes showing potential interactions with myelin-associated proteins. Understanding these relationships can guide therapeutic strategies targeting KLK6-related pathways.

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Product protocols

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Target data

Serine protease which exhibits a preference for Arg over Lys in the substrate P1 position and for Ser or Pro in the P2 position. Shows activity against amyloid precursor protein, myelin basic protein, gelatin, casein and extracellular matrix proteins such as fibronectin, laminin, vitronectin and collagen. Degrades alpha-synuclein and prevents its polymerization, indicating that it may be involved in the pathogenesis of Parkinson disease and other synucleinopathies. May be involved in regulation of axon outgrowth following spinal cord injury. Tumor cells treated with a neutralizing KLK6 antibody migrate less than control cells, suggesting a role in invasion and metastasis.

See full target information KLK6

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websiteProtocolBooklet

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human Kallikrein 6/KLK6 ELISA Kit

Sandwich ELISA - Human Kallikrein 6/KLK6 ELISA Kit (AB287163)

Sandwich ELISA - Human Kallikrein 6/KLK6 ELISA Kit (AB287163)

Sandwich ELISA - Human Kallikrein 6/KLK6 ELISA Kit (AB287163)

Sandwich ELISA - Human Kallikrein 6/KLK6 ELISA Kit (AB287163)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

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Primary Antibodies

Assay Kits

Secondary Antibodies

Product promise