AB287175

Human Kallikrein 7/KLK7 ELISA Kit

Recombinant
SimpleStep
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Human Kallikrein 7/KLK7 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Kallikrein 7/KLK7 with a sensitivity of 5.3 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

PRSS6, SCCE, KLK7, Kallikrein-7, hK7, Serine protease 6, Stratum corneum chymotryptic enzyme, hSCCE

4 Images

Sandwich ELISA - Human Kallikrein 7/KLK7 ELISA Kit (AB287175)

sELISA

Supplier Data

Sandwich ELISA - Human Kallikrein 7/KLK7 ELISA Kit (AB287175)

Interpolated concentrations of human Kallikrein 7/KLK7 in serum, plasma (citrate), plasma (heparin), plasma (EDTA), and HaCaT cell culture supernatant.

Interpolated concentration of native Kallikrein 7/KLK7 was measured in duplicate at different sample concentrations. Undiluted samples are 50% serum, plasma (citrate), plasma (heparin), and plasma (EDTA), and HaCaT cell culture supernatant. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Supplier Data

Sandwich ELISA - Human Kallikrein 7/KLK7 ELISA Kit (AB287175)

Example of human Kallikrein 7/KLK7 standard curve in Sample Diluent NS.

Example of human Kallikrein 7/KLK7 standard curve. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human Kallikrein 7/KLK7 ELISA Kit (AB287175)

This kit detects both pro-form and cleaved (active) form of Kallikrein 7/KLK7.

Recombinant Human Kallikrein 7/KLK7 pro-form and cleaved form were measured in duplicate at 750 pg/mL. Interpolated values are plotted (mean +/- SD, n=2).

sELISA

Supplier Data

Sandwich ELISA - Human Kallikrein 7/KLK7 ELISA Kit (AB287175)

Interpolated concentrations of Kallikrein 7/KLK7 in normal human serum donors.

Serum of ten individual healthy human female donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Kallikrein 7/KLK7 concentration was determined to be 2,342.3 pg/mL with a range of 1393.1 – 4959.1 pg/mL.

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 5.3 pg/mL

Range

21.88 - 1400 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human Kallikrein 7/KLK7 ELISA kit (ab287175) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human Kallikrein 7/KLK7 protein in human serum, plasma, and cell culture supernatant samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Kallikrein 7/KLK7 with 5.3 pg/mL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Plasma", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "1.7" }, { "reproducibilityType": "Intra", "sample": "Plasma", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.4" } ]

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Recovery

[ { "sample": "Cell culture supernatant", "range": "98 - 119 %", "average": "= 109" }, { "sample": "Serum", "range": "90 - 101 %", "average": "= 94" }, { "sample": "EDTA Plasma", "range": "89 - 105 %", "average": "= 96" }, { "sample": "Heparin Plasma", "range": "92 - 115 %", "average": "= 102" }, { "sample": "Citrate plasma", "range": "85 - 98 %", "average": "= 91" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab287175-1x96Tests", "assetComponentDetails": [ { "size":"1 x 600 µL", "name":"10X Human Kallikrein 7/KLK7 Capture Antibody", "number":"AB287175-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Kallikrein 7/KLK7 Lyophilized Recombinant Protein", "number":"AB287175-CMP08", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Kallikrein 7/KLK7 Detector Antibody", "number":"AB287175-CMP03", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB287175-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB287175-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB287175-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB287175-CMP09", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB287175-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB287175-CMP06", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB287175-CMP11", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Kallikrein 7 also known as KLK7 is a serine protease enzyme active in the skin. The protein's mass is approximately 27 kDa. It helps in the desquamation process by breaking down corneodesmosomes which are cell structures that facilitate cell adhesion in the outer skin layer. Kallikrein 7 is highly expressed in the epidermis and various mucosal surfaces reflecting its essential role in maintaining skin barrier functions.

Biological function summary

KLK7 contributes to skin homeostasis and renewal processes. Its enzymatic action involves cleaving proteins like desmoglein and corneodesmosin which are part of the desmosomal complex. This activity promotes the natural shedding of skin cells a process important for skin turnover and repair. By regulating this balance KLK7 supports not only skin health but also its resilience against external stressors.

Pathways

KLK7 plays a significant role in processes such as the proteolytic cascade and inflammation. It is involved in the kallikrein-kinin system alongside proteins like KLK5 that modulate inflammatory responses and barrier function in skin. KLK7 also interacts with pathways related to tissue remodeling influencing processes like inflammation through cytokine activation.

KLK7 is linked to skin conditions like atopic dermatitis and psoriasis. These disorders often feature a disrupted skin barrier where KLK7-mediated proteolytic activity increases and may exacerbate inflammation. The relationship with inflammation also connects KLK7 to proteins such as KLK5 which can further intensify skin disorders when their activities are unregulated.

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Product protocols

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Target data

May catalyze the degradation of intercellular cohesive structures in the cornified layer of the skin in the continuous shedding of cells from the skin surface. Specific for amino acid residues with aromatic side chains in the P1 position. Cleaves insulin A chain at '14-Tyr-|-Gln-15' and insulin B chain at '6-Leu-|-Cys-7', '16-Tyr-|-Leu-17', '25-Phe-|-Tyr-26' and '26-Tyr-|-Thr-27'. Could play a role in the activation of precursors to inflammatory cytokines.

See full target information KLK7

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human Kallikrein 7/KLK7 ELISA Kit

Sandwich ELISA - Human Kallikrein 7/KLK7 ELISA Kit (AB287175)

Sandwich ELISA - Human Kallikrein 7/KLK7 ELISA Kit (AB287175)

Sandwich ELISA - Human Kallikrein 7/KLK7 ELISA Kit (AB287175)

Sandwich ELISA - Human Kallikrein 7/KLK7 ELISA Kit (AB287175)

Key facts

Detection method

Sample types

Reacts with

Assay type

Results type

Sensitivity

Range

Assay time

Assay Platform

Complementary Products

Primary Antibodies

Assay Kits

Assay Kits

Primary Antibodies

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise