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AB213804

Human Kallikrein 8 ELISA Kit

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Human Kallikrein 8 ELISA Kit is a Sandwich ELISA for the measurement of Human Kallikrein 8 in Human in Cell/Tissue Extracts, Cell Culture Media, Biofluids samples.

View Alternative Names

NRPN, PRSS19, TADG14, UNQ283/PRO322, KLK8, Kallikrein-8, hK8, Neuropsin, Ovasin, Serine protease 19, Serine protease TADG-14, Tumor-associated differentially expressed gene 14 protein, NP

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Sandwich ELISA - Human Kallikrein 8 ELISA Kit (AB213804)
  • sELISA

Supplier Data

Sandwich ELISA - Human Kallikrein 8 ELISA Kit (AB213804)

Human Kallikrein-8 ELISA Kit (ab213804) Standard Curve.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate, Cell culture supernatant, EDTA Plasma, Heparin Plasma, Serum, Tissue Homogenate

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

< 10 pg/mL

Range

156 - 10000 pg/mL

Assay time

3h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Complementary Products

Primary Antibodies

AB150395

Anti-Kallikrein 8/KLK8 antibody [EPR5752(2)]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kallikrein 8/KLK8 antibody [EPR5752(2)] (AB150395)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

The Human Kallikrein 8 (KLK8) Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213804) is designed for the quantitative measurement of Human Kallikrein-8 in cell culture supernatants, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).

The ELISA kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Kallikrein-8 has been pre-coated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Kallikrein-8 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human Kallikrein-8 amount of sample captured in plate.

Kallikrein-8 is a protein that in humans is encoded by the KLK8 gene. Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Alternate splicing of this gene results in four transcript variants encoding four different isoforms. The isoforms exhibit distinct patterns of expression that suggest roles in brain plasticity and ovarian cancer.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Sample 1", "replicates": 24, "mean": "1625 pg/mL", "standardDeviation": "95.9", "coefficientOfVariability": "5.9" }, { "reproducibilityType": "Inter", "sample": "Sample 2", "replicates": 24, "mean": "4190 pg/mL", "standardDeviation": "301.7", "coefficientOfVariability": "7.2" }, { "reproducibilityType": "Inter", "sample": "Sample 3", "replicates": 24, "mean": "6526 pg/mL", "standardDeviation": "417.7", "coefficientOfVariability": "6.4" }, { "reproducibilityType": "Intra", "sample": "Sample 1", "replicates": 16, "mean": "1468 pg/mL", "standardDeviation": "67.53", "coefficientOfVariability": "4.6" }, { "reproducibilityType": "Intra", "sample": "Sample 2", "replicates": 16, "mean": "3882 pg/mL", "standardDeviation": "260.1", "coefficientOfVariability": "6.7" }, { "reproducibilityType": "Intra", "sample": "Sample 3", "replicates": 16, "mean": "6352 pg/mL", "standardDeviation": "349.4", "coefficientOfVariability": "5.5" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab213804-1x96Tests", "assetComponentDetails": [ { "size":"1 x 100 µL", "name":"Avidin-Biotin-Peroxidase Complex (ABC)", "number":"AB213804-CMP10", "productcode":"" }, { "size":"1 x 10 mL", "name":"TMB Stop Solution", "number":"AB213804-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"Antibody Diluent Buffer", "number":"AB213804-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Human KLK8 coated Microplate (12 x 8 wells)", "number":"AB213804-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human KLK8 Standard (Lyophilized)", "number":"AB213804-CMP01", "productcode":"" }, { "size":"4 x 1 Unit", "name":"Adhesive Plate Seal", "number":"AB213804-CMP07", "productcode":"" }, { "size":"1 x 10 mL", "name":"TMB Color Developing Agent", "number":"AB213804-CMP05", "productcode":"" }, { "size":"1 x 100 µL", "name":"Biotinylated KLK8 Antibody", "number":"AB213804-CMP09", "productcode":"" }, { "size":"1 x 30 mL", "name":"Sample Diluent Buffer", "number":"AB213804-CMP02", "productcode":"" }, { "size":"1 x 20 mL", "name":"Wash Buffer (25X)", "number":"AB213804-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"ABC Diluent Buffer", "number":"AB213804-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Kallikrein 8 also known as KLK8 is a serine protease enzyme with a molecular mass of approximately 28 kDa. Alternative names include Neurosin and hK8. It is expressed in various tissues notably in the brain testis and skin. KLK8 plays a mechanical role in cleaving protein substrates in these regions aiding in maintaining cellular structures and processes through its proteolytic activity. Its expression is important in neuronal development making it significant in central nervous system function.
Biological function summary

KLK8 involves itself in tissue remodeling and neural plasticity. It interacts with extracellular matrix proteins contributing to cell migration and adhesion. Important in synaptic plasticity KLK8 supports memory formation and learning. KLK8 operates as part of proteolytic cascades and may overlap with other kallikreins in function. Its activity includes modulating neural and skin cells suggesting its importance in cellular differentiation and growth.

Pathways

KLK8 takes part in the kallikrein-kinin system and extracellular matrix degradation pathways. It interacts with proteins such as KLK3 (prostate-specific antigen) within these pathways although KLK3 is more associated with prostate tissue. KLK8's function in these pathways ensures proper maintenance and remodeling of tissue-specific extracellular environments. These activities highlight its relevance in maintaining physiological balance and cellular responses in its primary tissues of expression.

KLK8 links with neurological conditions and certain skin disorders. Altered levels or dysfunctions of KLK8 correlate with disorders like Alzheimer's disease indicating its potential role in amyloid processing. Similar associations exist with skin disorders like psoriasis where KLK8 influences inflammatory response. Research also explores its connection with the protein amyloid-beta contributing to an understanding of its involvement in neurodegenerative diseases. Understanding these interactions can further illuminate potential therapeutic targets for treating these conditions.
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Product protocols

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Target data

Serine protease which is capable of degrading a number of proteins such as casein, fibrinogen, kininogen, fibronectin and collagen type IV. Also cleaves L1CAM in response to increased neural activity. Induces neurite outgrowth and fasciculation of cultured hippocampal neurons. Plays a role in the formation and maturation of orphan and small synaptic boutons in the Schaffer-collateral pathway, regulates Schaffer-collateral long-term potentiation in the hippocampus and is required for memory acquisition and synaptic plasticity. Involved in skin desquamation and keratinocyte proliferation. Plays a role in the secondary phase of pathogenesis following spinal cord injury.
See full target information KLK8
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websiteProtocolBooklet
en
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