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AB284009

Human KAP1 ELISA Kit

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Human KAP1 ELISA Kit is a Sandwich ELISA for the measurement of Human KAP1 in Human in Biofluids, Cell Culture Media, Cell/Tissue Extracts samples.

View Alternative Names

SDR25C1, DHRS2, Dicarbonyl reductase HEP27, Protein D, Short chain dehydrogenase/reductase family 25C member 1, Protein SDR25C1

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Sandwich ELISA - Human KAP1 ELISA Kit (AB284009)
  • sELISA

Supplier Data

Sandwich ELISA - Human KAP1 ELISA Kit (AB284009)

Example of Human KAP1 standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Saliva, Plasma, Cell culture supernatant, Serum, Cell Lysate, Tissue

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.25 ng/mL

Range

0.391 - 25 ng/mL

Assay time

4h

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Complementary Products

Primary Antibodies

AB243871

Anti-KAP1 antibody [BL-248-2G6]

Recombinant

Immunocytochemistry - Anti-KAP1 antibody [BL-248-2G6] (AB243871)

  • 0
  • 0
Primary Antibodies

AB194801

Anti-DHRS2 antibody

Western blot - Anti-DHRS2 antibody (AB194801)

  • 0
  • 0
Primary Antibodies

AB243870

Anti-KAP1 (phospho S824) antibody [BL-246-7B5]

Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAP1 (phospho S824) antibody [BL-246-7B5] (AB243870)

  • 0
  • 0
Primary Antibodies

AB245380

Anti-KMT1A / SUV39H1 antibody

Immunoprecipitation - Anti-KMT1A / SUV39H1 antibody (AB245380)

  • 4
  • 1
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human KAP1 ELISA Kit (ab284009) is designed for detection of KAP-1 in human plasma, serum, saliva, cell culture, and cell lysate samples. This assay employs a quantitative sandwich enzyme immunoassay technique that measures human KAP-1 in approximately 4 hours.

A polyclonal antibody specific for human KAP-1 has been pre-coated onto a 96-well microplate with removable strips. KAP-1 in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for human KAP-1, which is recognized by a streptavidin-peroxidase (SP) conjugate.The color development is stopped and the intensity of the color is measured.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 60, "mean": null, "standardDeviation": null, "coefficientOfVariability": "10.2" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 60, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.9" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

KAP1 also known as TRIM28 or TIF1β is a multifunctional protein involved in transcriptional regulation. It has a molecular weight of approximately 87 kDa. This protein is expressed in a wide variety of human tissues where it plays a role as a co-repressor for KRAB domain-containing zinc finger proteins. KAP1 recruits various epigenetic modifiers to silence transcription and this activity depends on its phosphorylation status. You might explore KAP1 using KAP1 ELISA kits and study its post-translational modifications like phosphorylation in various conditions.
Biological function summary

KAP1 acts as a scaffold protein facilitating the assembly of large protein complexes that include chromatin remodelers and histone deacetylases. It is significant in maintaining chromatin structure and mediating gene silencing. KAP1 phosphorylation serves as a switch between its roles in transcriptional repression and activation. This protein also interacts with heterochromatin protein 1 (HP1) and other TRIM family proteins playing an important role in genomic stability by controlling gene expression and repair mechanisms.

Pathways

KAP1 participates actively in p53 and DNA damage response pathways. In the p53 pathway KAP1 regulates genes involved in the cell cycle and apoptosis acting in conjunction with the p53 protein. Within the DNA damage response KAP1 modulates the repair of double-strand breaks by interacting with proteins like ATM kinase influencing cellular sensitivity to genotoxic stress. These pathways highlight its importance in maintaining cellular homeostasis and response to external stimuli.

KAP1 has known connections with cancer and neurological disorders due to its regulatory effects on gene expression and genome stability. In cancer aberrant KAP1 activity can lead to unregulated cell proliferation and survival often involving changes in its interaction with the p53 protein. In neurological disorders dysregulation of KAP1 is associated with impaired neural development and neurodegeneration. Altogether these associations highlight KAP1 as a potential target for therapeutic interventions in related diseases.
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Product protocols

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Target data

NADPH-dependent oxidoreductase which catalyzes the reduction of dicarbonyl compounds. Displays reductase activity in vitro with 3,4-hexanedione, 2,3-heptanedione and 1-phenyl-1,2-propanedione as substrates (PubMed : 16685466). May function as a dicarbonyl reductase in the enzymatic inactivation of reactive carbonyls involved in covalent modification of cellular components (PubMed : 16685466). Also displays a minor hydroxysteroid dehydrogenase activity toward bile acids such as ursodeoxycholic acid (UDCA) and isoursodeoxycholic acid (isoUDCA), which makes it unlikely to control hormone levels (PubMed : 16685466). Doesn't show any activity in vitro with retinoids and sugars as substrates (PubMed : 16685466). Attenuates MDM2-mediated p53/TP53 degradation, leading to p53/TP53 stabilization and increased transcription activity, resulting in the accumulation of MDM2 and CDKN1A/p21 (PubMed : 20547751). Reduces proliferation, migration and invasion of cancer cells and well as the production of ROS in cancer (PubMed : 29106393).
See full target information DHRS2
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websiteProtocolBooklet
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Product promise

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