AB315065

Human Kappa light chain ELISA Kit

SimpleStep
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Human Kappa light chain ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Kappa light chain with a sensitivity of 2.1 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader

View Alternative Names

Immunoglobulin kappa constant, Ig kappa chain C region, Ig kappa chain C region AG, Ig kappa chain C region CUM, Ig kappa chain C region EU, Ig kappa chain C region OU, Ig kappa chain C region ROY, Ig kappa chain C region TI, IGKC

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Sandwich ELISA - Human Kappa light chain ELISA Kit (AB315065)

sELISA

Supplier Data

Sandwich ELISA - Human Kappa light chain ELISA Kit (AB315065)

Interpolated concentration of native Kappa light chain was measured in duplicate at different sample concentrations. Undiluted samples are 1 : 1,000,000 serum, plasma (citrate), plasma (EDTA) and plasma (heparin). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS + 1X Enhancer.

sELISA

Supplier Data

Sandwich ELISA - Human Kappa light chain ELISA Kit (AB315065)

Example of human Kappa light chain standard curve. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human Kappa light chain ELISA Kit (AB315065)

Interpolated concentration of native Kappa light chain was measured in duplicate at different sample concentrations. Undiluted samples are 1 : 25,000 human milk. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS + 1X Enhancer.

sELISA

Supplier Data

Sandwich ELISA - Human Kappa light chain ELISA Kit (AB315065)

Serum of ten individual healthy human male donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Kappa light chain concentration was determined to be 412.2 µg/mL with a range of 257.1 – 723.1 µg/mL.

sELISA

Supplier Data

Sandwich ELISA - Human Kappa light chain ELISA Kit (AB315065)

Representative image of Human Kappa light chain ELISA Kit (ab315065)

Components shown from left to right :

- Sample diluent NS (2 bottles)

- 10X Human Kappa light chain Capture Antibody

- 10X Human Kappa light chain Detector Antibody

- Human Kappa light chain Lyophilised Purified Protein (2 vials)

- 50X Cell Extraction Enhancer Solution (ab193971)

- Stop Solution

- TMB solution

- 10X Wash Buffer PT (ab206977)

- Antibody Diluent 4BC

SimpleStep Pre-Coated 96-Well Microplate (ab206978) - shown behind bottles / vials.

Plate Seals - shown underneath bottles / vials.

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the ELISA kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit.

Key facts

Detection method

Colorimetric

Sample types

Serum, Heparin Plasma, Citrate plasma, EDTA Plasma, Milk

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 2.1 pg/mL

Range

15.625 - 1000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human Kappa light chain SimpleStep ELISA kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Kappa light chain protein in Serum, Hep Plasma, Cit Plasma, EDTA Plasma, Milk. Quantitate Human Kappa light chain with 2.1 pg/ml sensitivity.

SimpleStep ELISA^® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA^® protocol summary in the image section for further details. Our SimpleStep ELISA^® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA^® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA^® kits.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.9" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "1.53" } ]

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Recovery

[ { "sample": "Serum", "range": "93 - 115 %", "average": "= 104" }, { "sample": "Heparin Plasma", "range": "110 - 114 %", "average": "= 112" }, { "sample": "Citrate plasma", "range": "103 - 106 %", "average": "= 104" }, { "sample": "EDTA Plasma", "range": "97 - 108 %", "average": "= 104" }, { "sample": "Milk", "range": "98 - 107 %", "average": "= 102" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab315065-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB315065-CMP09", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB315065-CMP06", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB315065-CMP05", "productcode":"" }, { "size":"2 x 50 mL", "name":"Sample Diluent NS", "number":"AB315065-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Substrate", "number":"AB315065-CMP08", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Kappa light chain Capture Antibody", "number":"AB315065-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Kappa light chain Detector Antibody", "number":"AB315065-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Kappa light chain Lyophilized Recombinant Protein", "number":"AB315065-CMP03", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BC", "number":"AB315065-CMP04", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB315065-CMP11", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Pre-coated 96-Well Microplate", "number":"AB315065-CMP10", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The kappa light chain alternatively known as Ig kappa chain is a component of immunoglobulins which are important for immune response. This protein has a molecular mass of roughly 25 kDa. It predominantly expresses in B lymphocytes where it pairs with heavy chains to form antibodies. Its expression is indicative of mature B cell functions serving as a marker for certain types of immune cells. The kappa light chain can also be detected outside of cells circulating in the blood and other bodily fluids.

Biological function summary

Kappa light chains are integral to the structure and function of antibodies. As part of the immunoglobulin molecule these chains contribute to antigen specificity by combining with heavy chains. Together they form the antigen-binding site. The kappa light chain is not part of a multiprotein complex; rather it directly participates in the antibody response. The presence of either kappa or lambda light chains can determine the particularities of antibody characteristics.

Pathways

Kappa light chains are elements of the immunological pathways notably the adaptive immune response. They interact with other proteins like immunoglobulin heavy chains to neutralize pathogens. The constant region of the kappa chain plays a role in determining the isotype of immunoglobulins which affects how antibodies mediate immune effector functions. Cross-linking of surface immunoglobulin by antigens can trigger B cell activation an essential step in the adaptive immune system.

Kappa light chains associate closely with multiple myeloma and certain types of lymphomas. Overproduction of kappa light chains in these diseases results in high levels detectable in the serum or urine which can be used diagnostically as markers. Also dysregulation of kappa chains correlates with autoimmune disorders whereby the immune system incorrectly targets its tissues. In disorders like these the interplay between kappa and lambda chains often arises showing imbalance between the two as a sign of abnormal B cell activity.

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Product protocols

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Target data

Constant region of immunoglobulin light chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed : 20176268, PubMed : 22158414). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed : 17576170, PubMed : 20176268).

See full target information IGKC

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websiteProtocolBooklet

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Product promise

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Human Kappa light chain ELISA Kit

Sandwich ELISA - Human Kappa light chain ELISA Kit (AB315065)

Sandwich ELISA - Human Kappa light chain ELISA Kit (AB315065)

Sandwich ELISA - Human Kappa light chain ELISA Kit (AB315065)

Sandwich ELISA - Human Kappa light chain ELISA Kit (AB315065)

Sandwich ELISA - Human Kappa light chain ELISA Kit (AB315065)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

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Product promise