Human Kappa light chain ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Kappa light chain with a sensitivity of 2.1 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
Images
Key facts
- Detection method
- Colorimetric
- Sample types
- Serum, Heparin Plasma, Citrate plasma, EDTA Plasma, Milk
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 15.625 - 1000 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 2.1 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Constant region of immunoglobulin light chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed:20176268, PubMed:22158414). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed:17576170, PubMed:20176268).
Alternative names
Immunoglobulin kappa constant, Ig kappa chain C region, Ig kappa chain C region AG, Ig kappa chain C region CUM, Ig kappa chain C region EU, Ig kappa chain C region OU, Ig kappa chain C region ROY, Ig kappa chain C region TI, IGKC
What's included?
Recommended products
Human Kappa light chain ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Kappa light chain with a sensitivity of 2.1 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
Key facts
- Detection method
- Colorimetric
- Sample types
- Serum, Heparin Plasma, Citrate plasma, EDTA Plasma, Milk
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 15.625 - 1000 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 2.1 pg/mL
- General recovery
- 104%
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Serum | n 8 | C.V. 2.9 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Serum | n 3 | C.V. 1.53 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 104 | Range 93 - 115 % |
Sample type Heparin Plasma | Average % = 112 | Range 110 - 114 % |
Sample type Citrate plasma | Average % = 104 | Range 103 - 106 % |
Sample type EDTA Plasma | Average % = 104 | Range 97 - 108 % |
Sample type Milk | Average % = 102 | Range 98 - 107 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human Kappa light chain SimpleStep ELISA kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Kappa light chain protein in Serum, Hep Plasma, Cit Plasma, EDTA Plasma, Milk. Quantitate Human Kappa light chain with 2.1 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The kappa light chain alternatively known as Ig kappa chain is a component of immunoglobulins which are important for immune response. This protein has a molecular mass of roughly 25 kDa. It predominantly expresses in B lymphocytes where it pairs with heavy chains to form antibodies. Its expression is indicative of mature B cell functions serving as a marker for certain types of immune cells. The kappa light chain can also be detected outside of cells circulating in the blood and other bodily fluids.
- Biological function summary
Kappa light chains are integral to the structure and function of antibodies. As part of the immunoglobulin molecule these chains contribute to antigen specificity by combining with heavy chains. Together they form the antigen-binding site. The kappa light chain is not part of a multiprotein complex; rather it directly participates in the antibody response. The presence of either kappa or lambda light chains can determine the particularities of antibody characteristics.
- Pathways
Kappa light chains are elements of the immunological pathways notably the adaptive immune response. They interact with other proteins like immunoglobulin heavy chains to neutralize pathogens. The constant region of the kappa chain plays a role in determining the isotype of immunoglobulins which affects how antibodies mediate immune effector functions. Cross-linking of surface immunoglobulin by antigens can trigger B cell activation an essential step in the adaptive immune system.
- Associated diseases and disorders
Kappa light chains associate closely with multiple myeloma and certain types of lymphomas. Overproduction of kappa light chains in these diseases results in high levels detectable in the serum or urine which can be used diagnostically as markers. Also dysregulation of kappa chains correlates with autoimmune disorders whereby the immune system incorrectly targets its tissues. In disorders like these the interplay between kappa and lambda chains often arises showing imbalance between the two as a sign of abnormal B cell activity.
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4 product images
Sandwich ELISA - Human Kappa light chain ELISA Kit (ab315065)
Example of human Kappa light chain standard curve. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human Kappa light chain ELISA Kit (ab315065)
Interpolated concentration of native Kappa light chain was measured in duplicate at different sample concentrations. Undiluted samples are 1:1,000,000 serum, plasma (citrate), plasma (EDTA) and plasma (heparin). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS + 1X Enhancer.
Sandwich ELISA - Human Kappa light chain ELISA Kit (ab315065)
Serum of ten individual healthy human male donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Kappa light chain concentration was determined to be 412.2 µg/mL with a range of 257.1 – 723.1 µg/mL.
Sandwich ELISA - Human Kappa light chain ELISA Kit (ab315065)
Interpolated concentration of native Kappa light chain was measured in duplicate at different sample concentrations. Undiluted samples are 1:25,000 human milk. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS + 1X Enhancer.
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