Human KIM1 ELISA Kit (TIM-1)

Product details

The human KIM1 ELISA (Enzyme-Linked Immunosorbent Assay) kit is a complete kit for the quantitative measurement of KIM1 protein in urine.

The ELISA kit is ultra sensitive, reliably measuring as little as 1.2 pg/ml of Kim1. This enables the use of reduced input sample and results in reduced matrix interference. It is high throughput with results in <2 hours from up to 38 samples in duplicate. The kit uses a monoclonal antibody to KIM-1 immobilized on a microtiter plate to bind the KIM-1 in the standards or sample. After a short incubation the excess sample or standard is washed out and a biotinylated monoclonal antibody to KIM-1 is added. This antibody binds to the KIM-1 captured on the plate. After a short incubation the excess antibody is washed out and Streptavidin conjugated to Horseradish peroxidase is added, which binds to the biotin on the monoclonal antibody. The plate is then incubated. Once the incubation is complete, excess conjugate is washed out and TMB substrate solution is added. An HRP-catalyzed reaction generates a blue color in the solution. Stop solution is added to stop the substrate reaction. The resulting yellow color is read at 450 nm. The amount of signal is directly proportional to the level of KIM-1 in the sample.

Kim1 is an early biomarker for kidney injury or disease. It is the most highly upregulated protein in the proximal tubule of the injured kidney. It exists in very low levels in normal kidneys but when upregulated during injury, it is detectable in urine in a wide variety of human diseases. KIM-1 is a potential biomarker for renal injury, which would suggest it has great importance in various kidney diseases and disorders such as chronic kidney disease, as well as acute tubular necrosis and acute kidney failure.