AB108876

Human Kininogen ELISA Kit (KNG)

Name: Human Kininogen ELISA Kit (KNG) (ab108876) | Abcam
Brand: Abcam Limited
SKU: AB108876

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(3 Publications)

Human Kininogen ELISA Kit (KNG) is a Sandwich ELISA for the measurement of Human Kininogen (KNG) in Human in Biofluids samples.

View Alternative Names

BDK, KNG, KNG1, Kininogen-1, Alpha-2-thiol proteinase inhibitor, Fitzgerald factor, High molecular weight kininogen, Williams-Fitzgerald-Flaujeac factor, HMWK

1 Images

Sandwich ELISA - Human Kininogen ELISA Kit (KNG) (AB108876)

sELISA

Supplier Data

Sandwich ELISA - Human Kininogen ELISA Kit (KNG) (AB108876)

Representative Standard Curve Using ab108876.

Key facts

Detection method

Colorimetric

Sample types

Saliva, Urine, Cerebral Spinal Fluid, Milk, Plasma, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 59 pg/mL

Range

0.391 - 25 ng/mL

Assay time

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Abcam's Kininogen (KNG) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Kininogen in plasma, urine, saliva, milk, cerebrospinal fluid and cell culture supernatants.

A Kininogen specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Kininogen specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Kininogen captured in plate.

Get results in 90 minutes with Human HMW Kininogen ELISA Kit (ab189574) from our SimpleStep ELISA^® range.

The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "9.9" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.5" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

Multi

Storage information

Please refer to protocols

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The kininogen is a protein involved in various biological processes and also known as high molecular weight kininogen (HMWK). This protein which has a mass of approximately 120 kDa circulates in the plasma. Kininogen is synthesized by the liver and endothelial cells where it exists as HMWK. It serves as a precursor to kinins like bradykinin which are important in regulating blood pressure and inflammation.

Biological function summary

Kininogen participates in the kallikrein-kinin system. It plays an important role in blood coagulation and the inflammatory response. HMWK is not part of a complex but interacts with factors such as prekallikrein and Factor XII. By serving as a co-factor HMWK facilitates the activation of these molecules which progresses the coagulation cascade and inflammatory pathways.

Pathways

The kininogen functions within the kallikrein-kinin and coagulation systems. It interacts closely with enzymes like kallikrein which cleaves kininogen to release bradykinin assisting in vasodilation and increasing vascular permeability. Additionally HMWK is tethered to the intrinsic pathway of coagulation by activation of Factor XII which links it to proteins involved in blood clot formation.

The kininogen is associated with hereditary angioedema and bradykinin-mediated edema. Abnormalities in its function can lead to excessive bradykinin release. This condition involves excessive swelling due to alterations in vascular permeability. Moreover the protein is linked to chronic inflammatory diseases through its connection with the bradykinin receptor and related peptides.

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Product protocols

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Target data

Kininogens are inhibitors of thiol proteases. HMW-kininogen plays an important role in blood coagulation by helping to position optimally prekallikrein and factor XI next to factor XII; HMW-kininogen inhibits the thrombin- and plasmin-induced aggregation of thrombocytes. LMW-kininogen inhibits the aggregation of thrombocytes. LMW-kininogen is in contrast to HMW-kininogen not involved in blood clotting.. Bradykinin. The active peptide bradykinin is a potent vasodilatator that is released from HMW-kininogen shows a variety of physiological effects : (A) influence in smooth muscle contraction, (B) induction of hypotension, (C) natriuresis and diuresis, (D) decrease in blood glucose level, (E) it is a mediator of inflammation and causes (E1) increase in vascular permeability, (E2) stimulation of nociceptors (4E3) release of other mediators of inflammation (e.g. prostaglandins), (F) it has a cardioprotective effect (directly via bradykinin action, indirectly via endothelium-derived relaxing factor action).

See full target information KNG1

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Movement disorders : official journal of the Movement Disorder Society 35:2101-2106 PubMed33179332

2020

Cerebrospinal Fluid Levels of Kininogen-1 Indicate Early Cognitive Impairment in Parkinson's Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Ioanna Markaki,Sofia Bergström,Panagiota Tsitsi,Julia Remnestål,Anna Månberg,Ellen Hertz,Wojciech Paslawski,Kimmo Sorjonen,Mathias Uhlén,Graziella Mangone,Stephanie Carvalho,Olivier Rascol,Wassilios G Meissner,Eloi Magnin,Ullrich Wüllner,Jean-Christophe Corvol,Peter Nilsson,Per Svenningsson

Frontiers in neurology 10:37 PubMed30804871

2019

Kininogen Level in the Cerebrospinal Fluid May Be a Potential Biomarker for Predicting Epileptogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Zou,Xinxin Wang,Ligang Huang,Juan Liu,Yingying Kong,Shengtian Li,Qinchi Lu

PloS one 12:e0170741 PubMed28122020

2017

iTRAQ-Based Proteomics Reveals Novel Biomarkers for Idiopathic Pulmonary Fibrosis.

Applications

ELISA

Species

Human

Rui Niu,Ying Liu,Ying Zhang,Yuan Zhang,Hui Wang,Yongbin Wang,Wei Wang,Xiaohui Li

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Human Kininogen ELISA Kit (KNG)

Sandwich ELISA - Human Kininogen ELISA Kit (KNG) (AB108876)

Key facts

Detection method

Sample types

Reacts with

Assay type

Results type

Sensitivity

Range

Assay time

Assay Platform

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Assay Kits

Reactivity data

Product details

Precision

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise