Human Kininogen ELISA Kit (KNG) is a Sandwich (quantitative) ELISA kit for the measurement of Human Kininogen (KNG) in Human in Saliva, Urine, Cerebral Spinal Fluid, Milk, Plasma, Serum samples.
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- Movement disorders : official journal of the Movement Disorder Society 35:2101-21062020Cerebrospinal Fluid Levels of Kininogen-1 Indicate Early Cognitive Impairment in Parkinson's Disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIoanna Markaki,Sofia Bergström,Panagiota Tsitsi,Julia Remnestål,Anna Månberg,Ellen Hertz,Wojciech Paslawski,Kimmo Sorjonen,Mathias Uhlén,Graziella Mangone,Stephanie Carvalho,Olivier Rascol,Wassilios G Meissner,Eloi Magnin,Ullrich Wüllner,Jean-Christophe Corvol,Peter Nilsson,Per SvenningssonPubMed 33179332
- Frontiers in neurology 10:372019Kininogen Level in the Cerebrospinal Fluid May Be a Potential Biomarker for Predicting Epileptogenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJing Zou et. al.PubMed 30804871
- PloS one 12:e01707412017iTRAQ-Based Proteomics Reveals Novel Biomarkers for Idiopathic Pulmonary Fibrosis.
Key facts
- Detection method
- Colorimetric
- Sample types
- Saliva, Urine, Cerebral Spinal Fluid, Milk, Plasma, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 1.563 - 100 ng/mL
- Assay time
- 4h
- Sensitivity
- = 64 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Kininogens are inhibitors of thiol proteases. HMW-kininogen plays an important role in blood coagulation by helping to position optimally prekallikrein and factor XI next to factor XII; HMW-kininogen inhibits the thrombin- and plasmin-induced aggregation of thrombocytes. LMW-kininogen inhibits the aggregation of thrombocytes. LMW-kininogen is in contrast to HMW-kininogen not involved in blood clotting. Bradykinin. The active peptide bradykinin is a potent vasodilatator that is released from HMW-kininogen shows a variety of physiological effects: (A) influence in smooth muscle contraction, (B) induction of hypotension, (C) natriuresis and diuresis, (D) decrease in blood glucose level, (E) it is a mediator of inflammation and causes (E1) increase in vascular permeability, (E2) stimulation of nociceptors (4E3) release of other mediators of inflammation (e.g. prostaglandins), (F) it has a cardioprotective effect (directly via bradykinin action, indirectly via endothelium-derived relaxing factor action).
Alternative names
BDK, KNG, KNG1, Kininogen-1, Alpha-2-thiol proteinase inhibitor, Fitzgerald factor, High molecular weight kininogen, Williams-Fitzgerald-Flaujeac factor, HMWK
What's included?
Recommended products
Human Kininogen ELISA Kit (KNG) is a Sandwich (quantitative) ELISA kit for the measurement of Human Kininogen (KNG) in Human in Saliva, Urine, Cerebral Spinal Fluid, Milk, Plasma, Serum samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Saliva, Urine, Cerebral Spinal Fluid, Milk, Plasma, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 1.563 - 100 ng/mL
- Assay time
- 4h
- Assay Platform
- Microplate
- Sensitivity
- = 64 pg/mL
- General recovery
- 96%
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 0 | mean - | SD - | C.V. 4.4 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Overall | n 0 | mean - | SD - | C.V. 10.1 |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- Multi
- Storage information
- Please refer to protocols
Notes
Abcam's Kininogen (KNG) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Kininogen in urine, saliva, milk, cerebrospinal fluid and cell culture supernatants.
A Kininogen specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Kininogen specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Kininogen captured in plate.
Get results in 90 minutes with Human HMW Kininogen ELISA Kit (Human HMW Kininogen ELISA Kit ab189574) from our SimpleStep ELISA® range.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The kininogen is a protein involved in various biological processes and also known as high molecular weight kininogen (HMWK). This protein which has a mass of approximately 120 kDa circulates in the plasma. Kininogen is synthesized by the liver and endothelial cells where it exists as HMWK. It serves as a precursor to kinins like bradykinin which are important in regulating blood pressure and inflammation.
- Biological function summary
Kininogen participates in the kallikrein-kinin system. It plays an important role in blood coagulation and the inflammatory response. HMWK is not part of a complex but interacts with factors such as prekallikrein and Factor XII. By serving as a co-factor HMWK facilitates the activation of these molecules which progresses the coagulation cascade and inflammatory pathways.
- Pathways
The kininogen functions within the kallikrein-kinin and coagulation systems. It interacts closely with enzymes like kallikrein which cleaves kininogen to release bradykinin assisting in vasodilation and increasing vascular permeability. Additionally HMWK is tethered to the intrinsic pathway of coagulation by activation of Factor XII which links it to proteins involved in blood clot formation.
- Associated diseases and disorders
The kininogen is associated with hereditary angioedema and bradykinin-mediated edema. Abnormalities in its function can lead to excessive bradykinin release. This condition involves excessive swelling due to alterations in vascular permeability. Moreover the protein is linked to chronic inflammatory diseases through its connection with the bradykinin receptor and related peptides.
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Sandwich ELISA - Human Kininogen ELISA Kit (KNG) (ab108876)
Representative Standard Curve Using ab108876.
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Product protocols
- Visit the general protocols
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