AB288589

Human Kisspeptin ELISA Kit

Recombinant
SimpleStep
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Human Kisspeptin ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Kisspeptin with a sensitivity of 5.472 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

PP5098, KISS1, Metastasis-suppressor KiSS-1, Kisspeptin-1

2 Images

Sandwich ELISA - Human Kisspeptin ELISA Kit (AB288589)

sELISA

Supplier Data

Sandwich ELISA - Human Kisspeptin ELISA Kit (AB288589)

Interpolated concentration of native Kisspeptin was measured in duplicate at different sample concentrations. Undiluted samples are 250 μg/mL human placenta extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

sELISA

Supplier Data

Sandwich ELISA - Human Kisspeptin ELISA Kit (AB288589)

Example of human Kisspeptin standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Tissue Extracts

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 5.472 pg/mL

Range

18.75 - 1200 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human Kisspeptin ELISA Kit (ab288589) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Kisspeptin protein in tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Kisspeptin with 5.472 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.6" }, { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.4" } ]

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Recovery

[ { "sample": "Tissue Extracts", "range": "95 - 110 %", "average": "= 104" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab288589-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB288589-CMP06", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB288589-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB288589-CMP12", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB288589-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB288589-CMP10", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BI", "number":"AB288589-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Kisspeptin Lyophilized Recombinant Protein", "number":"AB288589-CMP09", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Kisspeptin Capture Antibody", "number":"AB288589-CMP03", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB288589-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB288589-CMP05", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Kisspeptin Detector Antibody", "number":"AB288589-CMP04", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Kisspeptin also known as metastin is a peptide with significant roles in reproductive physiology. Found within the hypothalamus and several peripheral tissues it comprises a sequence of 54 amino acids and has a molecular mass of around 12.8 kDa. Kisspeptin binds to the G protein-coupled receptor GPR54 facilitating its regulatory functions. Widely expressed throughout the brain particularly in the arcuate nucleus and anteroventral periventricular nucleus it acts on neurons to influence GnRH secretion critically.

Biological function summary

Kisspeptin regulates the hypothalamic-pituitary-gonadal axis by promoting the release of gonadotropin-releasing hormone (GnRH). This stimulation enhances the synthesis and release of luteinizing hormone (LH) and follicle-stimulating hormone (FSH) from the pituitary gland key components in reproductive maturation and fertility. Kisspeptin integrates into the neuroendocrine control network interacting closely with neurotransmitters and signaling molecules to maintain reproductive homeostasis.

Pathways

Kisspeptin plays an instrumental role in the reproductive signaling cascade. It serves as a critical upstream regulator in the GnRH signaling pathway essential for the control of puberty onset and reproductive function. The kisspeptin-GPR54 axis interacts with related proteins like neurokinin B and dynorphin which modulate its effect on GnRH neurons. These interactions create an intricate regulatory network essential to reproductive hormone feedback loops.

Altered kisspeptin signaling can contribute to conditions such as hypogonadotropic hypogonadism and polycystic ovary syndrome (PCOS). Mutations or deficiencies in the kisspeptin receptor GPR54 have been linked to hypogonadotropic hypogonadism impacting sexual development and fertility. PCOS involves complex kisspeptin-related pathways alongside insulin signaling dysregulation illustrating its intersection with metabolic pathways and potential treatment targets. Kisspeptin modulation affects significant pathways related to reproductive health making it a target of interest for therapeutic intervention in these conditions.

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Product protocols

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Target data

Metastasis suppressor protein in malignant melanomas and in some breast cancers. May regulate events downstream of cell-matrix adhesion, perhaps involving cytoskeletal reorganization. Generates a C-terminally amidated peptide, metastin which functions as the endogenous ligand of the G-protein coupled receptor GPR54. Activation of the receptor inhibits cell proliferation and cell migration, key characteristics of tumor metastasis. Kp-10 is a decapeptide derived from the primary translation product, isolated in conditioned medium of first trimester trophoblast. Kp-10, but not other kisspeptins, increased intracellular Ca(2+) levels in isolated first trimester trophoblasts. Kp-10 is a paracrine/endocrine regulator in fine-tuning trophoblast invasion generated by the trophoblast itself. The receptor is also essential for normal gonadotropin-released hormone physiology and for puberty. The hypothalamic KiSS1/GPR54 system is a pivotal factor in central regulation of the gonadotropic axis at puberty and in adulthood.

See full target information KISS1

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human Kisspeptin ELISA Kit

Sandwich ELISA - Human Kisspeptin ELISA Kit (AB288589)

Sandwich ELISA - Human Kisspeptin ELISA Kit (AB288589)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Assay Kits

Secondary Antibodies

Reagents, Controls & Accessories

Primary Antibodies

Product promise