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AB193706

Human KLK6 ELISA Kit

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Human KLK6 ELISA Kit is a Sandwich ELISA for the measurement of Human KLK6 in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

PRSS18, PRSS9, KLK6, Kallikrein-6, Neurosin, Protease M, SP59, Serine protease 18, Serine protease 9, Zyme

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Sandwich ELISA - Human KLK6 ELISA Kit (AB193706)
  • sELISA

Supplier Data

Sandwich ELISA - Human KLK6 ELISA Kit (AB193706)

Example of standard curve.

Data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.6 ng/mL

Range

0.6 - 150 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Secondary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's KLK6 Human ELISA Kit (ab193706) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human KLK6 in serum, plasma, and cell culture supernatant.

This assay employs an antibody specific for human KLK6 coated on a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures KLK6 present in the samples. The wells are washed and biotinylated anti-human KLK6 antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of KLK6 bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" } ]
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Recovery

[ { "sample": "Serum", "range": "87 - 101 %", "average": "= 92.41" }, { "sample": "Plasma", "range": "73 - 106 %", "average": "= 87.99" }, { "sample": "Cell culture media", "range": "106 - 141 %", "average": "= 118.9" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab193706-1x96Tests", "assetComponentDetails": [ { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB193706-CMP04", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB193706-CMP02", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB193706-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB193706-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Pre-coated Human KLK6 Microplate (12 strips x 8 wells)", "number":"AB193706-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human KLK6 Standards (lyophilized)", "number":"AB193706-CMP08", "productcode":"" }, { "size":"1 x 200 µL", "name":"150X HRP-Streptavidin Concentrate", "number":"AB193706-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human KLK6 detection antibody", "number":"AB193706-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Kallikrein 6 also known as KLK6 is a serine protease with notable enzymatic properties. This protein part of the kallikrein family weighs approximately 25 kDa and exhibits chymotrypsin-like activity. KLK6 is highly expressed in the central nervous system and various body fluids such as cerebrospinal fluid and milk. Due to this distribution KLK6 may play roles in neurological and physiological processes.
Biological function summary

KLK6 participates in the degradation of extracellular matrix components. It functions within complex proteolytic cascades facilitating tissue remodeling and repair. Additionally KLK6 interacts with other kallikreins like kallikrein 3 showing intricate regulation within the kallikrein-related peptidase network. This interplay points toward its importance in modulating extracellular environment and cellular interactions.

Pathways

KLK6 engages in the neurogenic and inflammatory response pathways. It ties into MAPK signaling influencing processes such as cell proliferation and apoptosis. Through these pathways KLK6 associates with proteins like MMPs which modulate extracellular matrix dynamics. Its presence in these pathways positions KLK6 as a significant regulatory protein during cellular homeostasis and stress responses.

KLK6 correlates with neurodegenerative diseases like Alzheimer's and multiple sclerosis. Altered expression and activity levels of KLK6 have implications for disease progression and pathology. In Alzheimer's KLK6 may influence amyloid-beta processing connecting with proteins like APP. In multiple sclerosis KLK6 affects demyelination processes showing potential interactions with myelin-associated proteins. Understanding these relationships can guide therapeutic strategies targeting KLK6-related pathways.
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Product protocols

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Target data

Serine protease which exhibits a preference for Arg over Lys in the substrate P1 position and for Ser or Pro in the P2 position. Shows activity against amyloid precursor protein, myelin basic protein, gelatin, casein and extracellular matrix proteins such as fibronectin, laminin, vitronectin and collagen. Degrades alpha-synuclein and prevents its polymerization, indicating that it may be involved in the pathogenesis of Parkinson disease and other synucleinopathies. May be involved in regulation of axon outgrowth following spinal cord injury. Tumor cells treated with a neutralizing KLK6 antibody migrate less than control cells, suggesting a role in invasion and metastasis.
See full target information KLK6
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websiteProtocolBooklet
en
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Product promise

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