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AB314737

Human KPNA2 ELISA Kit

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Human KPNA2 ELISA Kit is a Sandwich ELISA for the measurement of Human KPNA2 in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

RCH1, SRP1, KPNA2, Importin subunit alpha-1, Karyopherin subunit alpha-2, RAG cohort protein 1, SRP1-alpha

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Sandwich ELISA - Human KPNA2 ELISA Kit (AB314737)
  • sELISA

Supplier Data

Sandwich ELISA - Human KPNA2 ELISA Kit (AB314737)

Example of standard curve using ab314737. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Serum, Plasma, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 2 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Complementary Products

Primary Antibodies

AB289858

Anti-KPNA2 antibody [EPR25248-95]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KPNA2 antibody [EPR25248-95] (AB289858)

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Proteins & Peptides

AB123205

Recombinant Human KPNA2 protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human KPNA2 protein (His tag N-Terminus) (AB123205)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human KPNA2 ELISA kit is an in vitro enzymelinked immunosorbent assay for the quantitative measurement of human KPNA2 in serum (human KPNA2 concentration is low in normal serum/plasma, and may not be detectable in this assay), plasma and cell culture supernatants. This assay employs an antibody specific for human KPNA2 coated on a 96-well plate.
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Recovery

[ { "sample": "Serum", "range": "70 - 86 %", "average": "= 77.04" }, { "sample": "Plasma", "range": "68 - 81 %", "average": "= 73.62" }, { "sample": "Cell culture media", "range": "83 - 109 %", "average": "= 96.82" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab314737-1x96Tests", "assetComponentDetails": [ { "size":"1 x 15 mL", "name":"5X Assay Diluent (Item E2)", "number":"AB314737-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human KPNA2 Antibody-coated ELISA Plate", "number":"AB314737-CMP08", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB314737-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human KPNA2 Detection Antibody", "number":"AB314737-CMP09", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB314737-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB314737-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human KPNA2 Protein Standard", "number":"AB314737-CMP10", "productcode":"" }, { "size":"1 x 200 µL", "name":"100X HRP-Streptavidin", "number":"AB314737-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

KPNA2 also known as Importin subunit alpha-1 functions as a facilitator for nuclear protein import. It has a mass of about 58 kDa and resides mainly in the cytoplasm and nucleus. This protein binds with specific nuclear localization signals on cargo proteins and assists in their transport into the nucleus through the nuclear pore complex. KPNA2 expression levels vary between tissues but it often appears in higher amounts in proliferative tissues.
Biological function summary

KPNA2 plays an important role in cellular processes by forming a complex with importin beta aiding nuclear-cytoplasmic transport. This transport system is important for regulating gene expression and maintaining cellular homeostasis. KPNA2 influences the correct localization of many signaling and transcription factors impacting numerous cellular functions including cell cycle progression and DNA repair.

Pathways

KPNA2 is a critical player in the nuclear import pathway and intersects with the cell cycle regulation pathway. It is closely associated with proteins such as importin beta which together facilitate the translocation of proteins into the nucleus. KPNA2's function in importing various regulators ensures proper cell cycle progression and responds to cellular stress signals.

KPNA2 is linked to several cancers particularly breast and ovarian cancer. Its overexpression often correlates with poor prognosis in these cancers suggesting a role in tumor progression. KPNA2 interacts with oncogenic proteins like MYC and AURKA in these pathological states influencing tumor growth and survival mechanisms.
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Product protocols

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Target data

Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1 (PubMed : 28991411, PubMed : 32130408, PubMed : 7604027, PubMed : 7754385). Binds specifically and directly to substrates containing either a simple or bipartite NLS motif (PubMed : 28991411, PubMed : 32130408, PubMed : 7604027, PubMed : 7754385). Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism (PubMed : 7604027, PubMed : 7754385). At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Mediator of PR-DUB complex component BAP1 nuclear import; acts redundantly with KPNA1 and Transportin-1/TNPO1 (PubMed : 35446349).
See full target information KPNA2
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Product promise

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