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AB313980

Human Kynureninase ELISA Kit

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(1 Publication)

Human Kynureninase ELISA Kit is a Sandwich ELISA for the measurement of Human Kynureninase in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

Kynureninase, L-kynurenine hydrolase, KYNU

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Sandwich ELISA - Human Kynureninase ELISA Kit (AB313980)
  • sELISA

Supplier Data

Sandwich ELISA - Human Kynureninase ELISA Kit (AB313980)

Example of standard curve using ab313980. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Serum, Plasma, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.61 ng/mL

Range

0.61 - 150 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Kynureninase ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Kynureninase in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human Kynureninase coated on a 96-well plate.

This ELISA kit shows no cross-reactivity with the following cytokines tested: human ANGPTL7, CD36, CLEC9a, CL-P1, Dectin-2, DLL4, DSCAM, EDIL3, ENPP-7, Enteropeptidase, FCRL3, FCRLB, FGF-3, FLRT1, FLRT2, GBA3, GDF-11, Glycoprotein V, Granzyme A, IGSF4B, IL-28 R alpha, LAMA4, LRRC4, LRRTM3, NG2, NQO-1, PCSK2, PILR-alpha, Plexin A4, POGLUT1, PRELP, Smad4, SOX15, SOX7, SOX9, Syntaxin 6, TROP2, TSLP R, UNC5H4.

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Recovery

[ { "sample": "Serum", "range": "96 - 140 %", "average": "= 119.8" }, { "sample": "Plasma", "range": "69 - 114 %", "average": "= 95.34" }, { "sample": "Cell culture media", "range": "81 - 120 %", "average": "= 100.4" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab313980-1x96Tests", "assetComponentDetails": [ { "size":"1 x 15 mL", "name":"5X Assay Diluent (Item E2)", "number":"AB313980-CMP05", "productcode":"" }, { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin", "number":"AB313980-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human Kynureninase Detection Antibody", "number":"AB313980-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human Kynureninase Protein Standard", "number":"AB313980-CMP10", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human Kynureninase Antibody-coated ELISA Plate", "number":"AB313980-CMP08", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB313980-CMP07", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB313980-CMP04", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB313980-CMP06", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

L-Kynurenine Hydrolase also known as L-Kynureninase is an enzyme with a mass of approximately 47 kDa. It acts as an important player in the kynurenine pathway by catalyzing the hydrolysis of L-kynurenine into anthranilic acid. This enzymatic activity is important for the degradation of tryptophan. L-Kynureninase is expressed in various tissues including the liver and brain reflecting its importance in systemic metabolic processes.
Biological function summary

L-Kynurenine Hydrolase participates in tryptophan catabolism affecting amino acid metabolism. It operates within the larger enzyme complex involved in the kynurenine pathway which is important for producing metabolites that have neurological and immunological functions. The activity of L-Kynureninase can influence the levels of pathway intermediates which are related to neurotransmitter synthesis and immune responses.

Pathways

This enzyme significantly contributes to the kynurenine pathway which is a major route for tryptophan metabolism. The pathway provides a link to the biosynthesis of nicotinamide adenine dinucleotide (NAD+) an essential coenzyme in cellular respiration. Proteins such as kynurenine 3-monooxygenase and aminocarboxymuconate semialdehyde decarboxylase are closely related to L-Kynureninase in these pathways interacting at different points in the metabolic sequence.

Altered L-Kynurenine Hydrolase activity is associated with conditions like neurodegenerative diseases and certain autoimmune disorders. For instance disruptions in its function can contribute to the development of neurological disorders such as Alzheimer's disease due to imbalanced neuroactive metabolite levels. It also has a connection with proteins like quinolinic acid a neurotoxin that may accumulate when L-Kynureninase activity is impaired linking it to potential damage in neural tissues.
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Product protocols

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Target data

Catalyzes the cleavage of L-kynurenine (L-Kyn) and L-3-hydroxykynurenine (L-3OHKyn) into anthranilic acid (AA) and 3-hydroxyanthranilic acid (3-OHAA), respectively. Has a preference for the L-3-hydroxy form. Also has cysteine-conjugate-beta-lyase activity.
See full target information Kynureninase
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Biology direct 19:132 PubMed39707545

2024

Integrated machine learning reveals the role of tryptophan metabolism in clear cell renal cell carcinoma and its association with patient prognosis.

Applications

Unspecified application

Species

Unspecified reactive species

Fan Li,Haiyi Hu,Liyang Li,Lifeng Ding,Zeyi Lu,Xudong Mao,Ruyue Wang,Wenqin Luo,Yudong Lin,Yang Li,Xianjiong Chen,Ziwei Zhu,Yi Lu,Chenghao Zhou,Mingchao Wang,Liqun Xia,Gonghui Li,Lei Gao
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Product promise

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