AB278114

Human L1CAM ELISA Kit

Recombinant
SimpleStep
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(1 Publication)

Human L1CAM ELISA Kit is a single-wash 90-min Simplestep used to quantify Human L1CAM with a sensitivity of 17.2 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

CD171, CAML1, MIC5, L1CAM, Neural cell adhesion molecule L1, N-CAM-L1, NCAM-L1

4 Images

Sandwich ELISA - Human L1CAM ELISA Kit (AB278114)

sELISA

Supplier Data

Sandwich ELISA - Human L1CAM ELISA Kit (AB278114)

Interpolated concentrations of native L1CAM in human serum and plasma.

The concentrations of L1CAM were measured in duplicates, interpolated from the L1CAM standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 25%, plasma (citrate) 25%, plasma (EDTA) 25%, and plasma (heparin) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean L1CAM concentration was determined to be 17.32 ng/mL in serum, 14.92 ng/mL in plasma (citrate), 15.41 ng/mL in plasma (EDTA), and 14.45 ng/mL plasma (heparin).

sELISA

Supplier Data

Sandwich ELISA - Human L1CAM ELISA Kit (AB278114)

Interpolated concentrations of native L1CAM in HeLa cell extract based on a 50 μg/mL extract load.

The concentrations of L1CAM were measured in duplicate and interpolated from the L1CAM standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean L1CAM concentration was determined to be 6.83 ng/mL in HeLa extract.

sELISA

Supplier Data

Sandwich ELISA - Human L1CAM ELISA Kit (AB278114)

12.5% Serum from ten individual healthy human female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean L1CAM concentration was determined to be 17.90 ng/mL with a range of 13.70 – 25.20 ng/mL.

sELISA

Supplier Data

Sandwich ELISA - Human L1CAM ELISA Kit (AB278114)

Example of human L1CAM standard curve in Sample Diluent NS.

The L1CAM standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell culture media, Heparin Plasma, Serum, Cell Lysate, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 17.2 pg/mL

Range

101.56 - 6500 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human L1CAM ELISA kit (ab278114) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human L1CAM protein in human serum, plasma, cell and tissue extract samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human L1CAM with 17.20 pg/mL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Neural cell adhesion molecule L1 (L1CAM) is involved in dynamics of cell adhesion and generation of transmembrane signals at tyrosine kinase receptors. Studies suggest L1CAM functions during human brain development. L1CAM is critical neuronal migration, axonal growth and fasciculation, and synaptogenesis processes. In the mature brain, L1CAM plays a role in the dynamics of neuronal structure and function, including synaptic plasticity. Human L1CAM shows 88%, 88%, 99% and 89% sequence homology with mouse, rat, monkey, and cow, respectively.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "8.8" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.3" } ]

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Recovery

[ { "sample": "Serum", "range": "83 - 97 %", "average": "= 90" }, { "sample": "EDTA Plasma", "range": "89 - 99 %", "average": "= 94" }, { "sample": "Cell culture media", "range": "100 - 102 %", "average": "= 101" }, { "sample": "Heparin Plasma", "range": "92 - 110 %", "average": "= 98" }, { "sample": "Citrate plasma", "range": "85 - 104 %", "average": "= 104" }, { "sample": "Tissue Extracts", "range": "90 - 99 %", "average": "= 94" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

L1CAM also known as neural cell adhesion molecule L1 is a transmembrane protein with a molecular weight of approximately 200-220 kDa. The L1CAM protein belongs to the immunoglobulin superfamily and is heavily glycosylated. It plays a significant role in cell adhesion processes within the nervous system. L1CAM is expressed in a variety of tissues including the brain kidney and peripheral nerves and is notably present in neuronal cells where it engages in cell-cell interactions. Researchers frequently use L1CAM IHC ELISA methods and specific fluorescent markers like Alexa Fluor 568 to study its expression patterns and localization.

Biological function summary

L1CAM facilitates neuronal migration axonal growth and synaptic plasticity. This protein participates in the formation and maintenance of neural networks. It interacts with other cell adhesion molecules and components of the extracellular matrix. L1CAM acts as an important modulator within neuronal signaling complexes which impacts the development and regeneration of the nervous system. Insights into its structural and cell interaction properties have made it a focus for understanding nervous system functions.

Pathways

L1CAM participates extensively in the MAPK and PI3K/AKT pathways. These pathways contribute to cellular growth survival and programmed cell death. The protein interacts with other molecules such as integrins and FGFRs to propagate signaling cascades important for cellular response mechanisms. These connections help modulate cytoskeletal dynamics influencing cellular adhesion and motility needed during brain development and response to injury.

L1CAM has been associated with hydrocephalus and some cancers. Mutations in the L1CAM gene can lead to L1 syndrome which includes a spectrum of neurological disorders particularly hydrocephalus. In cancer overexpression or altered regulation of L1CAM may correlate with tumor aggressiveness and metastatic potential. L1CAM's association with integrin and cadherin proteins underlines its relevance in pathological states and highlights a potential target for therapeutic interventions.

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Product protocols

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Target data

Neural cell adhesion molecule involved in the dynamics of cell adhesion and in the generation of transmembrane signals at tyrosine kinase receptors. During brain development, critical in multiple processes, including neuronal migration, axonal growth and fasciculation, and synaptogenesis. In the mature brain, plays a role in the dynamics of neuronal structure and function, including synaptic plasticity.

See full target information L1CAM

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Science advances 9:eadi3647 PubMed37713494

2023

ATP1A3 as a target for isolating neuron-specific extracellular vesicles from human brain and biofluids.

Applications

Unspecified application

Species

Unspecified reactive species

Yang You,Zhengrong Zhang,Nadia Sultana,Maria Ericsson,Yuka A Martens,Min Sun,Takahisa Kanekiyo,Seiko Ikezu,Scott A Shaffer,Tsuneya Ikezu

View all publications

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human L1CAM ELISA Kit

Sandwich ELISA - Human L1CAM ELISA Kit (AB278114)

Sandwich ELISA - Human L1CAM ELISA Kit (AB278114)

Sandwich ELISA - Human L1CAM ELISA Kit (AB278114)

Sandwich ELISA - Human L1CAM ELISA Kit (AB278114)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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