Human Lactate Dehydrogenase A (LDHA) ELISA Kit- Extracellular is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human Lactate Dehydrogenase A (LDHA) - Extracellular in Serum, EDTA Plasma, Heparin Plasma, Cell culture media samples.
Images
Key facts
- Detection method
- Colorimetric
- Sample types
- Serum, EDTA Plasma, Heparin Plasma, Cell culture media
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 262.5 - 8400 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 84.239 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Interconverts simultaneously and stereospecifically pyruvate and lactate with concomitant interconversion of NADH and NAD(+).
Alternative names
PIG19, LDHA, L-lactate dehydrogenase A chain, LDH-A, Cell proliferation-inducing gene 19 protein, LDH muscle subunit, Renal carcinoma antigen NY-REN-59, LDH-M
What's included?
Human Lactate Dehydrogenase A (LDHA) ELISA Kit- Extracellular is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human Lactate Dehydrogenase A (LDHA) - Extracellular in Serum, EDTA Plasma, Heparin Plasma, Cell culture media samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Serum, EDTA Plasma, Heparin Plasma, Cell culture media
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 262.5 - 8400 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 84.239 pg/mL
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Serum | n 8 | C.V. = 4.7 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Serum | n 3 | C.V. = 7.2 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 95 | Range 85 - 104 % |
Sample type EDTA Plasma | Average % = 114 | Range 104 - 121 % |
Sample type Heparin Plasma | Average % = 94 | Range 89 - 97 % |
Sample type Cell culture media | Average % = 98 | Range 92 - 104 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human Lactate Dehydrogenase A (LDHA) SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Lactate Dehydrogenase A (LDHA) protein in Serum, EDTA Plasma, Hep Plasma, Cell culture media. Quantitate Human Lactate Dehydrogenase A (LDHA) with 84.239 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibodyanalyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
Singlewash protocol reduces assay time to 90 minutes or less.
High sensitivity, specificity and reproducibility from superior antibodies.
Fully validated in biological samples.
96wells plate breakable into 12 x 8 wells strips, available in 15pack (10 x 96well plates).
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
Sandwich ELISA - Human Lactate Dehydrogenase A (LDHA) ELISA Kit- Extracellular (ab323926)
Serum of nine individual healthy human female donors was measured in duplicate. Interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). The mean Lactate Dehydrogenase A concentration was determined to be 118.5 ng/mL with a range of 6.67 – 261.8 ng/mL.
Sandwich ELISA - Human Lactate Dehydrogenase A (LDHA) ELISA Kit- Extracellular (ab323926)
Interpolated concentration of native Lactate Dehydrogenase A was measured in duplicate at different sample concentrations. Undiluted samples are as follows: serum 10%, plasma (EDTA) 20%, and plasma (heparin) 20%. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
Sandwich ELISA - Human Lactate Dehydrogenase A (LDHA) ELISA Kit- Extracellular (ab323926)
Example of human Lactate Dehydrogenase A standard curve. Background-subtracted data values (mean +/- SD) are graphed.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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