Human Lactate Dehydrogenase A (LDHA) ELISA Kit- Intracellular is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human Lactate Dehydrogenase A (LDHA) - Intracellular in Cell Lysate, Tissue Extracts samples.
Images
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell Lysate, Tissue Extracts
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 187.5 - 12000 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 62.422 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Interconverts simultaneously and stereospecifically pyruvate and lactate with concomitant interconversion of NADH and NAD(+).
Alternative names
PIG19, LDHA, L-lactate dehydrogenase A chain, LDH-A, Cell proliferation-inducing gene 19 protein, LDH muscle subunit, Renal carcinoma antigen NY-REN-59, LDH-M
What's included?
Human Lactate Dehydrogenase A (LDHA) ELISA Kit- Intracellular is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human Lactate Dehydrogenase A (LDHA) - Intracellular in Cell Lysate, Tissue Extracts samples.
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell Lysate, Tissue Extracts
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 187.5 - 12000 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 62.422 pg/mL
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Extract | n 8 | C.V. = 9.5 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Extract | n 3 | C.V. = 7.6 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell Lysate | Average % = 103 | Range 93 - 112 % |
Sample type Tissue Extracts | Average % = 100 | Range 95 - 103 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human Lactate Dehydrogenase A (LDHA) SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Lactate Dehydrogenase A (LDHA) protein in Cell Lysate, Tissue extracts. Quantitate Human Lactate Dehydrogenase A (LDHA) with 62.422 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibodyanalyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
Singlewash protocol reduces assay time to 90 minutes or less.
High sensitivity, specificity and reproducibility from superior antibodies.
Fully validated in biological samples.
96wells plate breakable into 12 x 8 wells strips, available in 16pack (10 x 96well plates).
Also available in a fully validated 384well format.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
Sandwich ELISA - Human Lactate Dehydrogenase A (LDHA) ELISA Kit- Intracellular (ab323927)
Interpolated concentration of native Lactate Dehydrogenase A was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 50 µg/mL SH-SY5Y cell extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.
Sandwich ELISA - Human Lactate Dehydrogenase A (LDHA) ELISA Kit- Intracellular (ab323927)
Example of human Lactate Dehydrogenase A standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human Lactate Dehydrogenase A (LDHA) ELISA Kit- Intracellular (ab323927)
Interpolated concentration of native Lactate Dehydrogenase A was measured in duplicate at different sample concentrations. Undiluted samples are as follows: brain cortex tissue extract 2.5 µg/mL, skeletal muscle tissue extract 1 µg/mL, and stomach tissue extract 10 µg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.
Sandwich ELISA - Human Lactate Dehydrogenase A (LDHA) ELISA Kit- Intracellular (ab323927)
Interpolated concentration of native Lactate Dehydrogenase A was measured in duplicate at different sample concentrations. Undiluted samples are as follows: MCF-7 cell extract 1 µg/mL and SH-SY5Y cell extract 50 µg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.
Sandwich ELISA - Human Lactate Dehydrogenase A (LDHA) ELISA Kit- Intracellular (ab323927)
Example of human Lactate Dehydrogenase A standard curve. Background-subtracted data values (mean +/- SD) are graphed.
Downloads
Product protocols
- Visit the general protocols
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