JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB183367

Human Lactate Dehydrogenase B / LDH-B ELISA Kit

Be the first to review this product! Submit a review

|

(6 Publications)

Human Lactate Dehydrogenase B / LDH-B ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Lactate Dehydrogenase B / LDH-B with a sensitivity of 8 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Cited in over 5 citations

View Alternative Names

L-lactate dehydrogenase B chain, LDH-B, LDH heart subunit, Renal carcinoma antigen NY-REN-46, LDH-H, LDHB

8 Images
ELISA - Human Lactate Dehydrogenase B / LDH-B ELISA Kit (AB183367)
  • ELISA

Supplier Data

ELISA - Human Lactate Dehydrogenase B / LDH-B ELISA Kit (AB183367)
Sandwich ELISA - Human Lactate Dehydrogenase B / LDH-B ELISA Kit (AB183367)
  • sELISA

Supplier Data

Sandwich ELISA - Human Lactate Dehydrogenase B / LDH-B ELISA Kit (AB183367)

Interpolated concentrations of LDHB in Human serum from 10 donors.

Serum from 10 apparently healthy male donors was measured in triplicate for LDHB. The mean LDHB concentration was determined to be 161 ng/mL with a range of 56 – 226 ng/mL.

Sandwich ELISA - Human Lactate Dehydrogenase B / LDH-B ELISA Kit (AB183367)
  • sELISA

Supplier Data

Sandwich ELISA - Human Lactate Dehydrogenase B / LDH-B ELISA Kit (AB183367)

Quantitation of LDHB expression in different cell lines.

Interpolated values of LDHB are plotted for the indicated cell lines based on an extract load of 20 μg/mL.

Sandwich ELISA - Human Lactate Dehydrogenase B / LDH-B ELISA Kit (AB183367)
  • sELISA

Supplier Data

Sandwich ELISA - Human Lactate Dehydrogenase B / LDH-B ELISA Kit (AB183367)

Interpolated concentrations of LDHB in Human serum and plasma titrations.

The concentrations of LDHB were interpolated from the LDHB standard curve and corrected for sample dilution. The mean LDHB concentration was determined to be 197 ng/mL in plasma (heparin) and 173 ng/mL in serum.

Sandwich ELISA - Human Lactate Dehydrogenase B / LDH-B ELISA Kit (AB183367)
  • sELISA

Supplier Data

Sandwich ELISA - Human Lactate Dehydrogenase B / LDH-B ELISA Kit (AB183367)

Example of LDHB standard curve diluted in 1X Cell Extraction PTR.

Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Lactate Dehydrogenase B / LDH-B ELISA Kit (AB183367)
  • sELISA

Supplier Data

Sandwich ELISA - Human Lactate Dehydrogenase B / LDH-B ELISA Kit (AB183367)

Titration of Human Heart and Human Liver homogenate extracts within the working range of the assay.

Raw data in duplicate measurements are plotted. Background is represented as the dotted black line.

Sandwich ELISA - Human Lactate Dehydrogenase B / LDH-B ELISA Kit (AB183367)
  • sELISA

Supplier Data

Sandwich ELISA - Human Lactate Dehydrogenase B / LDH-B ELISA Kit (AB183367)

Titration of HeLa and HepG2 extracts within the working range of the assay.

Raw data in duplicate measurements are plotted. Background is represented as the dotted black line.

Sandwich ELISA - Human Lactate Dehydrogenase B / LDH-B ELISA Kit (AB183367)
  • sELISA

Supplier Data

Sandwich ELISA - Human Lactate Dehydrogenase B / LDH-B ELISA Kit (AB183367)

Example of LDHB standard curve diluted in NS.

Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Tissue Extracts, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, Cell Lysate, EDTA Plasma

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 8 pg/mL

Range

0.312 - 20 ng/mL

Assay time

1h 30m

Assay Platform

Microplate

style
left-column

Complementary Products

Assay Kits

AB264617

Human CKM ELISA Kit

Recombinant|SimpleStep

ELISA - Human CKM ELISA Kit (AB264617)

  • 0
  • 0
Assay Kits

AB65393

LDH Assay Kit (Cytotoxicity)

Biochemical assay - LDH Assay Kit (Cytotoxicity) (AB65393)

  • 0
  • 0
Primary Antibodies

AB8227

Anti-beta Actin antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Western blot - Anti-beta Actin antibody - Loading Control (AB8227)

  • 5
  • 104
Primary Antibodies

AB32042

Anti-Cleaved Caspase-3 antibody [E83-77]

20ul selling size|RabMAb|Recombinant|KO Validated

Western blot - Anti-Cleaved Caspase-3 antibody [E83-77] (AB32042)

  • 4
  • 28
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Human Lactate Dehydrogenase B / LDH-B ELISA Kit ab183367 is a rapid single-wash 90-min Sandwich ELISA to measure Human Lactate Dehydrogenase B / LDH-B in cell culture supernatant, cell lysate, citrate plasma, EDTA plasma, heparin plasma, serum, tissue extracts. This SimpleStep sensitivity is 8 pg/mL.

How the assay works

Human Lactate Dehydrogenase B / LDH-B SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human Lactate Dehydrogenase B / LDH-B ELISA Kit ab183367 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Functional lactate dehydrogenase are homo or hetero tetramers composed of M and H protein subunits encoded by the LDH-A and LDH-B genes, respectively. The major isozymes of skeletal muscle and liver has four muscle (M) subunits; while the main isozymes for heart muscle contains four (H) subunits. The other variants contain both types of subunits. LDHB converts pyruvate to lactate under low oxygen conditions, while in the LDH-A converts lactate into pyruvate. Elevated levels of LDH indicate tissue breakdown in hemolysis. LDH can be used as a marker of myocardial infarction, peaking at days 3 and lasting through day 10. Other disorders indicated by elevated LDH include cancer, meningitis, encephalitis, acute pancreatitis, and HIV. Defects in LDHB are a cause of hereditary LDH-B deficiency; however LDH-B deficiency is usually asymptomatic.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

style
left-column

Precision

[ { "reproducibilityType": "Inter", "sample": "HeLa Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.7" }, { "reproducibilityType": "Intra", "sample": "HeLa Extract", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "1.9" } ]
style
left-column

Recovery

[ { "sample": "Serum", "range": "101 - 108 %", "average": "= 105" }, { "sample": "EDTA Plasma", "range": "107 - 115 %", "average": "= 110" }, { "sample": "Heparin Plasma", "range": "102 - 105 %", "average": "= 104" }, { "sample": "Citrate plasma", "range": "107 - 114 %", "average": "= 110" }, { "sample": "Cell culture media", "range": "108 - 109 %", "average": "= 108" }, { "sample": "Extraction Buffer", "range": "97 - 100 %", "average": "= 98" } ]
style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab183367-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB183367-CMP10", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB183367-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Lactate Dehydrogenase B / LDH-B Capture Antibody", "number":"AB183367-CMP08", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB183367-CMP06", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB183367-CMP11", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Lactate Dehydrogenase B / LDH-B Lyophilized Recombinant Protein", "number":"AB183367-CMP12", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB183367-CMP04", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB183367-CMP07", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Lactate Dehydrogenase B / LDH-B Detector Antibody", "number":"AB183367-CMP09", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB183367-CMP02", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB183367-CMP05", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB183367-CMP13", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Lactate Dehydrogenase B (LDH-B) also known as LDH is an enzyme that plays an important role in the interconversion of lactate to pyruvate. It belongs to the lactate dehydrogenase family which includes multiple isoenzymes. LDH-B has a molecular weight of approximately 36 kDa and commonly forms a tetramer with three other similar subunits. It is mainly expressed in heart and muscle tissues among others where it functions effectively.
Biological function summary

LDH-B is essential for cellular energy production processes. It catalyzes the conversion of pyruvate a byproduct of glycolysis into lactate while simultaneously converting NADH to NAD+. This reaction is significant under anaerobic conditions where mitochondria-based ATP production is limited. LDH-B does not function alone and can operate as part of the lactate dehydrogenase complex containing different subunit combinations.

Pathways

Lactate and pyruvate metabolism engage LDH-B facilitating the Cori cycle and the lactate shuttle. These pathways enable lactate transport between tissues such as muscle and liver and they are important in maintaining blood glucose levels and energy balance. LDH-B operates alongside its related isoenzymes such as LDH-A which predominantly act in specific tissues and conditions to optimize energy utilization.

Anomalous LDH-B activity associates with conditions like myocardial infarction and some cancer types. Enhanced expression or activity of LDH-B can indicate altered metabolic states in tumors connecting it as a potential marker for certain cancerous conditions. The relationship with other proteins such as the hypoxia-inducible factor (HIF) is notable especially in cancer where HIF can regulate LDH-B expression under low oxygen environments.
style
left-column

Product protocols

style
left-column

Target data

Interconverts simultaneously and stereospecifically pyruvate and lactate with concomitant interconversion of NADH and NAD(+).
See full target information LDHB
style
left-column

Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Asian Pacific journal of cancer prevention : APJCP 26:757-766 PubMed40156391

2025

The Synergistic and Anticancer Potential of Withania Somnifera (Ashwagandha) Ethanol Extract as an Adjuvant with Doxorubicin in MCF7 Breast Cancer Cell Line.

Applications

Unspecified application

Species

Unspecified reactive species

Emad M Elzayat,Ghada E Elsamahy,Ghada H Mansour,Ahmed A El-Sherif,Nourhan Hassan

Archives of endocrinology and metabolism 68:e230381 PubMed39420894

2024

Modified-release gliclazide acutely improves recovery but causes undesirable blood glucose decrease after a resistance exercise session in healthy adults: a pilot study for a randomized clinical trial.

Applications

Unspecified application

Species

Unspecified reactive species

Jocelito B Martins,Diego Zanella,Ramiro B Nunes,Pilar S Collado,Alexandre Machado Lehnen

Archives of toxicology 97:1997-2014 PubMed37210688

2023

Involvement of necroptosis in the selective toxicity of the natural compound (±) gossypol on squamous skin cancer cells in vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Lisa Haasler,Claudia von Montfort,Arun Kumar Kondadi,Mathias Golombek,Lara Ebbert,Chantal-Kristin Wenzel,Wilhelm Stahl,Andreas S Reichert,Peter Brenneisen

Combinatorial chemistry & high throughput screening 26:1204-1213 PubMed35838232

2022

Penehyclidine Hydrochloride Protects Rat Cardiomyocytes from Ischemia- Reperfusion Injury by Platelet-derived Growth Factor-B.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Lu,Congna Zi,Liang Zhang,Boqun Cui,Ling Li,Jun Ma

Bioengineered 12:2676-2687 PubMed34115550

2021

Overexpression of PLK1 relieved the myocardial ischemia-reperfusion injury of rats through inducing the mitophagy and regulating the p-AMPK/FUNDC1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Shan Mao,Shuning Tian,Xianghong Luo,Ming Zhou,Zheng Cao,Ji Li

Molecular medicine reports 22:5454-5462 PubMed33174008

2020

Silencing of long non‑coding RNA HRIM protects against myocardial ischemia/reperfusion injury via inhibition of NF‑κB signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Li Niu,Yunquan Zhao,Shumei Liu,Weiwei Pan
View all publications
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com