JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB200015

Human Lactoferrin ELISA Kit

5

(1 Review)

|

(20 Publications)

Human Lactoferrin ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Lactoferrin with a sensitivity of 48.8 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 15 citations

View Alternative Names

GIG12, LF, LTF, Lactotransferrin, Lactoferrin, Growth-inhibiting protein 12, Talalactoferrin

8 Images
ELISA - Human Lactoferrin ELISA Kit (AB200015)
  • ELISA

Supplier Data

ELISA - Human Lactoferrin ELISA Kit (AB200015)
Sandwich ELISA - Human Lactoferrin ELISA Kit (AB200015)
  • sELISA

Supplier Data

Sandwich ELISA - Human Lactoferrin ELISA Kit (AB200015)

Serum from ten individual healthy human female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Lactoferrin concentration was determined to be 473.8 ng/mL with a range of 315.2 – 852.7 ng/mL.

Sandwich ELISA - Human Lactoferrin ELISA Kit (AB200015)
  • sELISA

Supplier Data

Sandwich ELISA - Human Lactoferrin ELISA Kit (AB200015)

Example of human Lactoferrin standard curve in Sample Diluent NS. The Lactoferrin standard curve was prepared. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Lactoferrin ELISA Kit (AB200015)
  • sELISA

Supplier Data

Sandwich ELISA - Human Lactoferrin ELISA Kit (AB200015)

Linearity of dilution.

Native Lactoferrin was measured in human milk, urine, and saliva samples in a 2-fold dilution series. Sample dilutions are made in Sample Diluent NS.

Native purified Lactoferrin protein was spiked into RPMI 1640 cell culture media containing 10% fetal bovine serum and measured in a 2-fold dilution series. Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human Lactoferrin ELISA Kit (AB200015)
  • sELISA

Supplier Data

Sandwich ELISA - Human Lactoferrin ELISA Kit (AB200015)

Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.

Native Lactoferrin was measured in the following biological samples in a 2-fold dilution series. Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human Lactoferrin ELISA Kit (AB200015)
  • sELISA

Supplier Data

Sandwich ELISA - Human Lactoferrin ELISA Kit (AB200015)

Interpolated concentrations of native Lactoferrin in human serum and plasma samples.

The concentrations of Lactoferrin were measured in duplicates, interpolated from the Lactoferrin standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1 : 200, plasma (citrate) 1 : 200, plasma (heparin) 1 : 200, and plasma (EDTA) 1 : 200. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Lactoferrin concentration was determined to be 1,142 ng/mL in neat serum, 885.1 ng/mL in neat plasma (citrate), 1,054 ng/mL in neat plasma (heparin), and 581.4 ng/mL in neat plasma (EDTA).

Sandwich ELISA - Human Lactoferrin ELISA Kit (AB200015)
  • sELISA

Supplier Data

Sandwich ELISA - Human Lactoferrin ELISA Kit (AB200015)

Example of human Lactoferrin standard curve in Sample Diluent NS.

The Lactoferrin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Lactoferrin ELISA Kit (AB200015)
  • sELISA

Supplier Data

Sandwich ELISA - Human Lactoferrin ELISA Kit (AB200015)

Interpolated concentrations of native Lactoferrin in human milk (defatted), urine, and saliva samples.

The concentrations of Lactoferrin were measured in duplicates, interpolated from the Lactoferrin standard curves and corrected for sample dilution. Undiluted samples are as follows : milk 1 : 500,000, urine 1 : 20, and saliva 1 : 10,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Lactoferrin concentration was determined to be 4,021.6 μg/mL in neat milk (defatted), 0.033 μg/mL in neat urine, and 39.1 μg/mL in neat saliva.

Key facts

Detection method

Colorimetric

Sample types

Saliva, Urine, Cell culture media, Heparin Plasma, Citrate plasma, Milk, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 48.8 pg/mL

Range

156.3 - 10000 pg/mL

Assay time

1h 30m

Assay Platform

Microplate (12 x 8 well strips)

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Human Lactoferrin ELISA Kit ab200015 is a rapid single-wash 90-min Sandwich ELISA to measure Human Lactoferrin in cell culture media, citrate plasma, EDTA plasma, heparin plasma, milk, saliva, serum, urine. This SimpleStep sensitivity is 48.8 pg/mL.

How the assay works

Human Lactoferrin SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human Lactoferrin ELISA Kit ab200015 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-Lactoferrin antibody [EPR21853-197] - BSA and Azide free (Capture) ab259454
- Anti-Lactoferrin antibody [EPR21853-113] - BSA and Azide free (Detector) ab259454

Transferrins are iron binding transport proteins which can bind two Fe3+ ions in association with the binding of an anion, usually bicarbonate. Lactoferrin is a member of the transferrin family and is a major iron-binding and multifunctional protein found in exocrine fluids such as breast milk and mucosal secretions. High levels of Lactoferrin are found in saliva and tears, intermediate levels in serum and plasma, and low levels in urine. The canonical sequence of Lactoferrin (known as isoform 1) is 710 amino acids in length and runs at 78.2 kDa. Isoform Delta-Lactoferrin (or DeltaLF) is missing amino acids 1 – 44 of the canonical sequence. Mouse and rat Lactoferrin are 71% and 62% identical to human Lactoferrin, respectively.Lactoferrin possesses antibacterial, antiviral, anti-inflammatory, and immunoregulatory properties. The presence of Lactoferrin in neutrophils and the release of Lactoferrin during inflammation suggest that Lactoferrin is involved in phagocytic killing and immune responses. Additionally, Lactoferrin mediates bacteriostasis through its ability to sequester free iron and inhibit microbial growth. Lactoferrin also directs bactericidal properties leading to the release of lipopolysaccharides from the bacterial outer membrane. Furthermore, Lactoferrin may play a role in the regulation of bone growth because it has anabolic, differentiating, and anti-apoptotic effects on osteoblasts and can also inhibit osteoclastogenesis. Salivary Lactoferrin plays an important role in protecting the oral cavity against several oral pathogens including Streptococcus mutans. Additionally, increased levels of Lactoferrin in fecal samples is used as a marker of active inflammatory bowel disease (IBD).

style
left-column

Precision

[ { "reproducibilityType": "Inter", "sample": "Human Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.4" }, { "reproducibilityType": "Intra", "sample": "Human Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.1" } ]
style
left-column

Recovery

[ { "sample": "Milk", "range": "99 - 118 %", "average": "= 109" }, { "sample": "Serum", "range": "100 - 105 %", "average": "= 102" }, { "sample": "EDTA Plasma", "range": "105 - 110 %", "average": "= 108" }, { "sample": "Urine", "range": "99 - 100 %", "average": "= 99" }, { "sample": "Saliva", "range": "113 - 126 %", "average": "= 120" }, { "sample": "Cell culture media", "range": "86 - 86 %", "average": "= 86" }, { "sample": "Heparin Plasma", "range": "105 - 110 %", "average": "= 106" }, { "sample": "Citrate plasma", "range": "109 - 111 %", "average": "= 110" } ]
style
left-column

What's included?

{ "values": { "10x96Tests": { "sellingSize": "10 x 96 Tests", "publicAssetCode":"ab200015-10x96Tests", "assetComponentDetails": [ { "size":"1 x 120 mL", "name":"Stop Solution", "number":"AB200015-CMP06" }, { "size":"1 x 200 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB200015-CMP10" }, { "size":"1 x 120 mL", "name":"TMB Development Solution", "number":"AB200015-CMP07" }, { "size":"2 x 10 Vial", "name":"Human Lactoferrin Lyophilized Purified Protein", "number":"AB200015-CMP08" }, { "size":"10 x 6 mL", "name":"Antibody Diluent 5BI", "number":"AB200015-CMP01" }, { "size":"2 x 250 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB200015-CMP04" }, { "size":"1 x 6000 µL", "name":"10X Human Lactoferrin Capture Antibody", "number":"AB200015-CMP02" }, { "size":"1 x 6000 µL", "name":"10X Human Lactoferrin Detector Antibody", "number":"AB200015-CMP03" }, { "size":"10 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB200015-CMP05" }, { "size":"10 x 1 Unit", "name":"Plate Seals", "number":"AB200015-CMP09" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab200015-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB200015-CMP06", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB200015-CMP10", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB200015-CMP04", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB200015-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB200015-CMP09", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Lactoferrin Capture Antibody", "number":"AB200015-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Lactoferrin Lyophilized Purified Protein", "number":"AB200015-CMP08", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BI", "number":"AB200015-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Lactoferrin Detector Antibody", "number":"AB200015-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB200015-CMP05", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Lactoferrin also known as lactotransferrin is an iron-binding glycoprotein with a molecular weight of about 80 kDa. It predominantly exists in mammalian milk particularly in high concentrations in colostrum and is also found in various epithelial secretions such as saliva tears and nasal fluids. The liver and neutrophils also produce lactoferrin and it plays a significant role in the body's primary defense system. As an antibacterial agent lactoferrin sequesters free iron to limit bacterial growth and disrupt biofilms assisting in the protection against microbial invasions.
Biological function summary

Lactoferrin exhibits multiple functions beyond its antimicrobial properties. It contributes to immunomodulation by binding to specific receptors on immune cells enhancing phagocytosis and influencing cytokine production. Also it possesses anti-inflammatory and antioxidant properties. Lactoferrin interacts with cellular components including glycosaminoglycans and lipoproteins impacting the activity and expression of proteins within signaling and metabolic pathways. This multifaceted role places it as a critical participant in both innate and adaptive immune responses although it does not form part of a stable protein complex.

Pathways

Lactoferrin plays a significant role in the iron metabolism and immune response pathways. It tightly interacts with the transferrin receptor modulating iron uptake and distribution across tissues which helps prevent iron overload and oxidative stress. Furthermore lactoferrin links with the toll-like receptor pathways particularly TLR4 which enables the modulation of innate immune responses against pathogens. By influencing nuclear factor kappa B (NF-kB) signaling lactoferrin impacts inflammation and immune responses working together with proteins such as ferritin and hepcidin.

Lactoferrin has relevancy to diseases like anemia of chronic disease and inflammatory bowel disease (IBD). Its role in regulating iron homeostasis makes it essential for preventing anemia associated with chronic inflammation. Lactoferrin mitigates dysregulated immune responses in IBD potentially reducing inflammation and promoting gut health. Disease-modifying properties of lactoferrin connect it to glycoproteins like transferrin and inflammatory mediators demonstrating its potential therapeutic importance.
style
left-column

Product protocols

style
left-column

Target data

Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate.. Lactotransferrin. Major iron-binding and multifunctional protein found in exocrine fluids such as breast milk and mucosal secretions (PubMed : 11179314, PubMed : 12693969, PubMed : 14573629, PubMed : 1599934, PubMed : 3169987, PubMed : 6802759). Has antimicrobial activity, which depends on the extracellular cation concentration (PubMed : 6802759). Antimicrobial properties include bacteriostasis, which is related to its ability to sequester free iron and thus inhibit microbial growth, as well as direct bactericidal properties leading to the release of lipopolysaccharides from the bacterial outer membrane (PubMed : 11179314, PubMed : 12693969, PubMed : 14573629, PubMed : 1599934, PubMed : 3169987, PubMed : 6802759). Can also prevent bacterial biofilm development in P.aeruginosa infection (PubMed : 12037568). Has weak antifungal activity against C.albicans (PubMed : 11083624). Has anabolic, differentiating and anti-apoptotic effects on osteoblasts and can also inhibit osteoclastogenesis, possibly playing a role in the regulation of bone growth (PubMed : 15166119). Promotes binding of species C adenoviruses to epithelial cells, promoting adenovirus infection (PubMed : 17079302). Can inhibit papillomavirus infections (PubMed : 17481742). Stimulates the TLR4 signaling pathway leading to NF-kappa-B activation and subsequent pro-inflammatory cytokine production while also interfering with the lipopolysaccharide (LPS)-stimulated TLR4 signaling (PubMed : 20345905). Inhibits neutrophil granulocyte migration to sites of apoptosis, when secreted by apoptotic cells (PubMed : 19033648). Stimulates VEGFA-mediated endothelial cell migration and proliferation (PubMed : 16842782). Binds heparin, chondroitin sulfate and possibly other glycosaminoglycans (GAGs) (PubMed : 9359845). Also binds specifically to pneumococcal surface protein A (PspA), the lipid A portion of bacterial lipopolysaccharide (LPS), lysozyme and DNA (PubMed : 9359845).. Lactoferricin binds to the bacterial surface and is crucial for the bactericidal functions. Has some antiviral activity against papillomavirus infection (PubMed : 17481742). N-terminal region shows strong antifungal activity against C.albicans (PubMed : 11083624). Contains two BBXB heparin-binding consensus sequences that appear to form the predominate functional GAG-binding site.. Kaliocin-1. Has antimicrobial activity and is able to permeabilize different ions through liposomal membranes.. Lactoferroxin-A. Has opioid antagonist activity (PubMed : 1369293). Shows preference for mu-receptor (PubMed : 1369293).. Lactoferroxin-B. Has opioid antagonist activity (PubMed : 1369293). Shows higher degrees of preference for kappa-receptors than for mu-receptors (PubMed : 1369293).. Lactoferroxin-C. Has opioid antagonist activity (PubMed : 1369293). Shows higher degrees of preference for kappa-receptors than for mu-receptors (PubMed : 1369293).. The lactotransferrin transferrin-like domain 1 functions as a serine protease of the peptidase S60 family that cuts arginine rich regions (PubMed : 12535064). This function contributes to the antimicrobial activity (PubMed : 12535064). Shows a preferential cleavage at -Arg-Ser-Arg-Arg-|- and -Arg-Arg-Ser-Arg-|-, and of Z-Phe-Arg-|-aminomethylcoumarin sites (PubMed : 12535064).. Isoform DeltaLf. Transcription factor with antiproliferative properties and ability to induce cell cycle arrest (PubMed : 15222485). Binds to the DeltaLf response element found in the SKP1, BAX, DCPS, and SELENOH promoters (PubMed : 22320386).
See full target information LTF
style
left-column

Publications (20)

Recent publications for all applications. Explore the full list and refine your search

EBioMedicine 116:105721 PubMed40344717

2025

Faecal mucoprotein MUC2 is decreased in multiple sclerosis and is associated with mucin degrading bacteria.

Applications

Unspecified application

Species

Unspecified reactive species

Luke A Schwerdtfeger,Federico Montini,Tanuja Chitnis,Laura M Cox,Howard L Weiner

mBio 16:e0194324 PubMed39576068

2024

induces dose-dependent shock, organ dysfunction, and coagulopathy in a nonhuman primate critical care model.

Applications

Unspecified application

Species

Unspecified reactive species

Jeffrey R Strich,Marcos J Ramos-Benitez,Seth Warner,Heather Kendall,Sydney Stein,Andrew P Platt,Sabrina C Ramelli,Shelly J Curran,Izabella Lach,Kiana Allen,Ashley Babyak,Luis J Perez-Valencia,Mahnaz Minai,Junfeng Sun,Kevin M Vannella,Derron Alves,Richard Herbert,Daniel S Chertow

Children (Basel, Switzerland) 11: PubMed39334656

2024

Lactoferrin in Pediatric Chronic Kidney Disease and Its Relationship with Cardiovascular Risk.

Applications

Unspecified application

Species

Unspecified reactive species

Chun-Yi Ho,Pei-Chen Lu,Wei-Ling Chen,Wei-Ting Liao,Chien-Ning Hsu,You-Lin Tain

Antioxidants (Basel, Switzerland) 13: PubMed39334730

2024

Impact of Hypoxia on Neutrophil Degranulation and Inflammatory Response in Alpha-1 Antitrypsin Deficiency Patients.

Applications

Unspecified application

Species

Unspecified reactive species

María Magallón,Silvia Castillo-Corullón,Lucía Bañuls,Teresa Romero,Daniel Pellicer,Alberto Herrejón,María Mercedes Navarro-García,Cruz González,Francisco Dasí

Nutrients 16: PubMed39064803

2024

Digestive Profiles of Human Milk, Recombinant Human and Bovine Lactoferrin: Comparing the Retained Intact Protein and Peptide Release.

Applications

Unspecified application

Species

Unspecified reactive species

Bum Jin Kim,Russell F Kuhfeld,Joanna L Haas,Yanisa M Anaya,Raysa Rosario Martinez,Baidya Nath P Sah,Bella Breen,Kahler Newsham,Carrie-Anne Malinczak,David C Dallas

Scientific reports 14:14505 PubMed38914667

2024

Fluorescence-based reagent and spectrum-based optical reader for lactoferrin detection in tears: differentiating Sjögren's syndrome from non-Sjögren's dry eye syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Chia-Ying Tsai,Chitsung Hong,Min-Yen Hsu,Tso-Ting Lai,Ching-Wen Huang,Cheng-Yo Lu,Wei-Li Chen,Chao-Min Cheng

PloS one 18:e0291297 PubMed37992002

2023

Salivary concentrations of secretory leukocyte protease inhibitor and matrix metallopeptidase-9 following a single bout of exercise are associated with intensity and hydration status.

Applications

Unspecified application

Species

Unspecified reactive species

Karen Knipping,Shirley W Kartaram,Marc Teunis,Nicolaas P A Zuithoff,Nicole Buurman,Laura M'Rabet,Klaske van Norren,Renger Witkamp,Raymond Pieters,Johan Garssen

Scientific reports 13:4903 PubMed36966172

2023

The abundances of LTF and SOD2 in amniotic fluid are potential biomarkers of gestational age and preterm birth.

Applications

Unspecified application

Species

Unspecified reactive species

Te-Yao Hsu,Hsin-Hsin Cheng,Kuo-Chung Lan,Hsuan-Ning Hung,Yun-Ju Lai,Chih-Chang Tsai,Wen-Lang Fan,Sung-Chou Li

The Tohoku journal of experimental medicine 259:135-142 PubMed36476585

2022

Exploratory Study of Serum Lactoferrin and Anti-Lactoferrin Antibody Concentrations in Patients with Endometriosis.

Applications

Unspecified application

Species

Unspecified reactive species

Kaya Mori-Yamanaka,Fuminori Kimura,Akiko Nakamura,Tetsuro Hanada,Jun Kitazawa,Aina Morimune,Shunichiro Tsuji,Takashi Murakami

International journal of molecular sciences 23: PubMed35955814

2022

Possible Implications of Bacteriospermia on the Sperm Quality, Oxidative Characteristics, and Seminal Cytokine Network in Normozoospermic Men.

Applications

Unspecified application

Species

Unspecified reactive species

Eva Tvrdá,Daniel Lovíšek,Eliška Gálová,Marianna Schwarzová,Eva Kováčiková,Simona Kunová,Jana Žiarovská,Miroslava Kačániová
View all publications
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Complementary Products

Assay Kits

AB267798

Human Lysozyme ELISA Kit

Recombinant|SimpleStep

ELISA - Human Lysozyme ELISA Kit (AB267798)

  • 0
  • 0
Assay Kits

AB270204

Human Neutrophil Elastase ELISA Kit

Recombinant|SimpleStep

ELISA - Human Neutrophil Elastase ELISA Kit (AB270204)

  • 0
  • 0
Assay Kits

AB246539

Human MMP9 ELISA Kit

Recombinant|SimpleStep

ELISA - Human MMP9 ELISA Kit (AB246539)

  • 5
  • 1
Assay Kits

AB243675

Human alpha 1 Acid Glycoprotein ELISA Kit

Recombinant|SimpleStep

ELISA - Human alpha 1 Acid Glycoprotein ELISA Kit (AB243675)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com