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AB315047

Human LAIR1 ELISA Kit

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Human LAIR1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human LAIR1 with a sensitivity of 3.071 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

CD305, LAIR1, Leukocyte-associated immunoglobulin-like receptor 1, LAIR-1, hLAIR1

3 Images
Sandwich ELISA - Human LAIR1 ELISA Kit (AB315047)
  • sELISA

Supplier Data

Sandwich ELISA - Human LAIR1 ELISA Kit (AB315047)

Interpolated concentration of native LAIR1 was measured in duplicate at different sample concentrations. Undiluted samples are as follows : THP-1 cell culture supernatant 50% and urine 1.25%. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human LAIR1 ELISA Kit (AB315047)
  • sELISA

Supplier Data

Sandwich ELISA - Human LAIR1 ELISA Kit (AB315047)

Interpolated concentration of native LAIR1 was measured in duplicate at different sample concentrations. Undiluted samples are 25% serum, plasma (citrate), plasma (EDTA), and plasma (heparin). The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human LAIR1 ELISA Kit (AB315047)
  • sELISA

Supplier Data

Sandwich ELISA - Human LAIR1 ELISA Kit (AB315047)

Example of human LAIR1 standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Serum, Heparin Plasma, EDTA Plasma, Citrate plasma, Urine, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 3.071 pg/mL

Range

9.375 - 600 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human LAIR1 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of LAIR1 protein in Serum, Hep Plasma, Cit Plasma, EDTA Plasma, Urine, Cell culture supernatant. Quantitate Human LAIR1 with 3.071 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Human Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.5" }, { "reproducibilityType": "Inter", "sample": "Human Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.29" } ]
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Recovery

[ { "sample": "Serum", "range": "86 - 101 %", "average": "= 94" }, { "sample": "Heparin Plasma", "range": "93 - 105 %", "average": "= 100" }, { "sample": "EDTA Plasma", "range": "90 - 106 %", "average": "= 98" }, { "sample": "Citrate plasma", "range": "95 - 107 %", "average": "= 101" }, { "sample": "Urine", "range": "87 - 101 %", "average": "= 95" }, { "sample": "Cell culture supernatant", "range": "105 - 108 %", "average": "= 107" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

LAIR1 also known as Leukocyte-Associated Immunoglobulin-like Receptor 1 is a transmembrane protein with a molecular weight of approximately 32 kDa. It expresses mainly on immune cells including T cells B cells and myeloid cells. This receptor by binding to collagen plays a significant role in controlling immune cell signaling. It acts as an inhibitory receptor which helps in maintaining immune system balance and prevents excessive immune responses.
Biological function summary

LAIR1 functions by modulating immune responses and maintaining homeostasis in the immune system. It is a part of a complex pathway involved in inhibitory signaling. When LAIR1 binds to its ligand such as collagen it transduces a signal that reduces activation of immune cells. This inhibition ensures that immune cells do not become overactive which could otherwise lead to tissue damage.

Pathways

LAIR1 participates in immune checkpoint regulation and collagen interaction pathways. It interacts with proteins such as ITIM-containing molecules which transmit inhibitory signals essential for regulating immune cell activation. It also indirectly connects to the TGF-beta signaling pathway which is vital for controlling cell differentiation and immune regulation.

LAIR1 is of interest due to its connection to autoimmune diseases and certain cancers. In autoimmune diseases such as rheumatoid arthritis dysregulated LAIR1 signaling may contribute to inflammation. The protein is also linked to cancer where its inhibitory signaling might affect tumor immune evasion. The connection between LAIR1 and these conditions involves proteins like autoimmune-related genes and tumor-associated surface molecules.
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Product protocols

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Target data

Functions as an inhibitory receptor that plays a constitutive negative regulatory role on cytolytic function of natural killer (NK) cells, B-cells and T-cells. Activation by Tyr phosphorylation results in recruitment and activation of the phosphatases PTPN6 and PTPN11. It also reduces the increase of intracellular calcium evoked by B-cell receptor ligation. May also play its inhibitory role independently of SH2-containing phosphatases. Modulates cytokine production in CD4+ T-cells, down-regulating IL2 and IFNG production while inducing secretion of transforming growth factor beta. Down-regulates also IgG and IgE production in B-cells as well as IL8, IL10 and TNF secretion. Inhibits proliferation and induces apoptosis in myeloid leukemia cell lines as well as prevents nuclear translocation of NF-kappa-B p65 subunit/RELA and phosphorylation of I-kappa-B alpha/CHUK in these cells. Inhibits the differentiation of peripheral blood precursors towards dendritic cells.
See full target information LAIR1
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websiteProtocolBooklet
en
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