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AB277432

Human LAIR2 ELISA Kit

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Human LAIR2 ELISA Kit is a Sandwich ELISA for the measurement of Human LAIR2 in Human in Biofluids, Cell Culture Media samples.
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ELISA - Human LAIR2 ELISA Kit (AB277432)
  • ELISA

Supplier Data

ELISA - Human LAIR2 ELISA Kit (AB277432)

These standard curves are for demonstration only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 2.2 ng/mL

Range

1.966 - 480 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human LAIR2 ELISA Kit (ab277432) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human LAIR2 in serum (human LAIR2 concentration is low in normal serum/plasma, and may not be detectable in this assay), plasma and cell culture supernatants.

This assay employs an antibody specific for Human LAIR2 coated on a 96-well plate. Standards and samples are pipetted into the wells and Human LAIR2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human LAIR2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human LAIR2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" } ]
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Recovery

[ { "sample": "Serum", "range": "68 - 146 %", "average": "= 93.31" }, { "sample": "Plasma", "range": "69 - 115 %", "average": "= 90.25" }, { "sample": "Cell culture media", "range": "134 - 143 %", "average": "= 138.1" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab277432-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB277432-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Human LAIR2 coated Microplate", "number":"AB277432-CMP03", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB277432-CMP01", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent", "number":"AB277432-CMP04", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB277432-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Human LAIR2 Antibody", "number":"AB277432-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human LAIR2 standard protein (Lyophilized)", "number":"AB277432-CMP07", "productcode":"" }, { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin Concentrate", "number":"AB277432-CMP02", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Leukocyte-Associated Immunoglobulin-like Receptor 2 (LAIR2) also known as CD306 is a soluble protein with a mass of approximately 15.2 kDa. It can be found in serum where immune system regulation occurs. Structurally LAIR2 shares similarities with LAIR1 a membrane-bound protein although LAIR2 lacks a transmembrane domain. It acts by sequestering ligands that would interact with LAIR1 thereby modulating the immune system response.
Biological function summary

LAIR2 regulates immune cell function by inhibiting collagen-induced signaling that typically activates immune responses. LAIR2 functions independently without forming a complex with other cell surface receptors or signaling molecules. By binding to collagens this protein limits unnecessary immune activation acting as a checkpoint in immune regulation and maintaining tolerance.

Pathways

Multiple immune regulatory pathways involve LAIR2 including the collagen signaling pathway that modulates immune cell activity. LAIR2 is related to LAIR1 since they both respond to collagen interactions in the immune system. Disruption in these pathways may affect immune homeostasis linking LAIR2 directly to collagen-mediated modulation.

Disturbances in LAIR2 expression have links with autoimmune disorders and chronic inflammatory conditions. The protein is of particular interest in studies on systemic lupus erythematosus where altered LAIR2 levels can influence disease progression. Additionally LAIR2 connections with LAIR1 suggest potential implications in fibrotic diseases identifying it as a therapeutic target for modulating immune responses.
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Product protocols

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Target data

See full target information LAIR2

Alternative Names

CD306, LAIR2, Leukocyte-associated immunoglobulin-like receptor 2, LAIR-2
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Product promise

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